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基質(zhì)Gla蛋白(MGP)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Matrix Gla Protein (MGP) ,etc. by FLIA (Flow Luminescence Immunoassay)

MGLAP; GIG36; Cell growth-inhibiting gene 36 protein

(注:單次混測多因子不超過8個指標 )

  • 基質(zhì)Gla蛋白(MGP)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • 基質(zhì)Gla蛋白(MGP)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒用于檢測基質(zhì)Gla蛋白(MGP)等多因子檢測試劑盒(流式熒光發(fā)光法),經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質(zhì)交叉反應檢測,因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的基質(zhì)Gla蛋白(MGP)等多因子檢測試劑盒(流式熒光發(fā)光法)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 78-104 89
EDTA plasma(n=5) 86-93 90
heparin plasma(n=5) 79-96 89

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的基質(zhì)Gla蛋白(MGP)等多因子檢測試劑盒(流式熒光發(fā)光法),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中基質(zhì)Gla蛋白(MGP)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 91-98% 81-91% 80-97% 90-104%
EDTA plasma(n=5) 94-101% 95-104% 94-101% 80-96%
heparin plasma(n=5) 78-97% 78-97% 91-105% 93-101%

穩(wěn)定性

經(jīng)測定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備;
2. 加樣(標準品、樣本、磁珠)標準品或樣本100μL及磁珠10μL,
    37°C酶標板振蕩器孵育90分鐘;
3. 磁吸甩干,加檢測溶液A100μL,37°C酶標板振蕩器孵育60分鐘;
4. 磁吸洗板3次;
5. 加檢測溶液B100μL,37°C振動孵育30分鐘;
6. 磁吸洗板3次;
7. 加鞘液100μL,旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將基質(zhì)Gla蛋白(MGP)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠,制成固相載體,向微孔中分別加入標準品或標本以及磁珠,其中的基質(zhì)Gla蛋白(MGP)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結合,然后加入生物素化的基質(zhì)Gla蛋白(MGP)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體,將未結合的生物素化抗體洗凈后,加入PE標記的親和素,再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的基質(zhì)Gla蛋白(MGP)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關。

贈品

相關產(chǎn)品

編號 適用物種:Rattus norvegicus (Rat,大鼠) 應用(僅供研究使用,不用于臨床診斷!)
RPB477Ra02 基質(zhì)Gla蛋白(MGP)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB477Ra01 基質(zhì)Gla蛋白(MGP)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB477Ra02 基質(zhì)Gla蛋白(MGP)多克隆抗體 WB; IHC; ICC; IP.
PAB477Ra01 基質(zhì)Gla蛋白(MGP)多克隆抗體 WB; IHC; ICC; IP.
LAB477Ra71 基質(zhì)Gla蛋白(MGP)多克隆抗體(生物素標記) WB; IHC; ICC.
LAB477Ra81 基質(zhì)Gla蛋白(MGP)多克隆抗體(異硫氰酸熒光素標記) WB; IHC; ICC; IF.
SEB477Ra 基質(zhì)Gla蛋白(MGP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB477Ra 基質(zhì)Gla蛋白(MGP)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSB477Ra01 基質(zhì)Gla蛋白(MGP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志 參考文獻
Nephrology Dialysis Transplantation Magnesium prevents phosphate-induced calcification in human aortic vascular smooth muscle cells [PubMed: PMC3611891]
Maturitas. Bone metabolism regulators and arterial stiffness in postmenopausal women [Pubmed: 23916080]
The Egyptian Rheumatologist Osteoprotegerin (OPG) and Matrix Gla protein (MGP) in rheumatoid arthritis patients: Relation to disease activity [ScienceDirect: S1110116414000076]
Toxicology Reports Canola and hydrogenated soybean oils accelerate ectopic bone formation induced by implantation of bone morphogenetic protein in mice [Researchgate:Source]
Lipids in Health and Disease Markers of increased cardiovascular risk in patients with chronic kidney disease [Biomedcentral:Source]
Human &amp; Veterinary Medicine The behavior of circulating matrix Gla protein, matrix metalloproteinase-9 and nitrotyrosine in patients with varicose veins [Hvm:Source]
Pol Arch Med Wewn Effect of Vitamin K2 on progression of atherosclerosis and vascular calcification in nondialyzed patients with chronic kidney disease stages 3-5 [PubMed: 26176325]
Lipids Health Dis. Markers of increased atherosclerotic risk in patients with chronic kidney disease: a preliminary study [pmc:PMC4739105]
Kidney Blood Press Res. Plasma Desphospho-Uncarboxylated Matrix Gla Protein as a Marker of Kidney Damage and Cardiovascular Risk in Advanced Stage of Chronic Kidney Disease [Pubmed:27100101]
Archives of?Oral?Biology Matrix-Gla Protein rs4236 [A/G] gene polymorphism and serum and GCF levels of MGP in patients with subgingival dental calculus [pubmed:27348051]
Cell?Death?&?Disease Mesenchymal stromal cells-derived matrix Gla protein contribute to the alleviation of experimental colitis [Pubmed:29880866]
Urology High concentration of calcium promotes mineralization in NRK52E cells via inhibiting the expression of matrix Gla protein [Pubmed:29935264]
Clinica?Chimica?Acta Serum total matrix Gla protein: Reference interval in healthy adults and variations in patients with vascular and osteoarticular diseases [Pubmed: 30597137]
Biological?Trace?Element?Research Altered Mineral Metabolism and Disequilibrium Between Calcification Promoters and Inhibitors in Chronic Hemodialysis Patients [Pubmed: 30847765]
Renal Failure Relationship of matrix Gla protein and vitamin K with vascular calcification in hemodialysis patients [Pubmed: 31538831]
Heliyon Matrix Gla protein maintains normal and malignant hematopoietic progenitor cells by interacting with bone morphogenetic protein-4 [Pubmed: 32322728]
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