基質(zhì)金屬蛋白酶13(MMP13)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Matrix Metalloproteinase 13 (MMP13) ,etc. by FLIA (Flow Luminescence Immunoassay)
CLG3; Collagenase 3
（注：單次混測多因子不超過8個(gè)指標(biāo) ）
- 編號(hào)LMA099Eq
- 物種Equus caballus; Equine (Horse，馬) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長3.5h
- 檢測范圍0.05-50ng/mL
- 靈敏度最小可檢測劑量小于等于0.017 ng/mL.
- 樣本類型Serum, plasma, tissue homogenates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 8指標(biāo)數(shù) 7指標(biāo)數(shù) 6指標(biāo)數(shù) 5指標(biāo)數(shù) 4指標(biāo)數(shù) 3指標(biāo)數(shù) 2指標(biāo)數(shù)1指標(biāo)數(shù)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測基質(zhì)金屬蛋白酶13(MMP13)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的基質(zhì)金屬蛋白酶13(MMP13)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 98-105 | 101 |
| EDTA plasma(n=5) | 94-104 | 101 |
| heparin plasma(n=5) | 94-101 | 97 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的基質(zhì)金屬蛋白酶13(MMP13)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中基質(zhì)金屬蛋白酶13(MMP13)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-91% | 96-105% | 99-105% | 88-97% |
| EDTA plasma(n=5) | 78-101% | 91-101% | 78-94% | 88-98% |
| heparin plasma(n=5) | 79-90% | 98-105% | 84-101% | 89-96% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠）標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL，
37°C酶標(biāo)板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標(biāo)板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動(dòng)孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實(shí)驗(yàn)原理
將基質(zhì)金屬蛋白酶13(MMP13)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠，其中的基質(zhì)金屬蛋白酶13(MMP13)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的基質(zhì)金屬蛋白酶13(MMP13)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的基質(zhì)金屬蛋白酶13(MMP13)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。
贈(zèng)品
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Equus caballus; Equine (Horse，馬) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA099Eq01 | 基質(zhì)金屬蛋白酶13(MMP13)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA099Eq01 | 基質(zhì)金屬蛋白酶13(MMP13)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA099Eq21 | 基質(zhì)金屬蛋白酶13(MMP13)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA099Eq | 基質(zhì)金屬蛋白酶13(MMP13)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA099Eq | 基質(zhì)金屬蛋白酶13(MMP13)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA099Eq | 基質(zhì)金屬蛋白酶13(MMP13)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease | Hyaluronan reduces inflammation in experimental arthritis by modulating TLR-2 and TLR-4 cartilage expression [ScienceDirect: S0925443911001323] |
| Journal of Physiology and Biochemistry | Protective effects of curcumin, α-lipoic acid, and N-acetylcysteine against carbon tetrachloride-induced liver fibrosis in rats [SpringerLink: h875w73171w2147w] |
| FEBS Journal | Adenosine A2a Receptor Activation and Hyaluronan Fragment Inhibition Reduce Inflammation in Mouse Articular Chondrocytes Stimulated with Il-1beta [Wiley: source] |
| Arthritis & Rheumatism | Interruption of glycosphingolipid synthesis enhances osteoarthritis development in mice [Wiley: source] |
| Gene | Hyaluronan in part mediates IL-1beta-induced inflammation in mouse chondrocytes by up-regulating CD44 receptors [ScienceDirect: S0378111911007347] |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source] |
| Arthritis Rheumatol | Identification of Alpha 2 Macroglobulin (A2M) as a master inhibitor of cartilage degrading factors that attenuates post-traumatic osteoarthritis progression. [Pubmed: 24578232] |
| Arthritis & Rheumatology | Identification of Alpha 2 Macroglobulin (A2M) as a master inhibitor of cartilage degrading factors that attenuates post-traumatic osteoarthritis progression [Onlinelibrary: art.38576] |
| Thromb Haemost. | Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke [Pubmed:24671655] |
| Arthritis & Rheumatol | Identification of α2-Macroglobulin as a Master Inhibitor of Cartilage-Degrading Factors That Attenuates the Progression of Posttraumatic Osteoarthritis [Pubmed:24578232] |
| Inflammation.? | Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis [Pubmed:25448261] |
| Cellular Physiology and Biochemistry | Inverse Regulation of Early and Late Chondrogenic Differentiation by Oxygen Tension Provides Cues for Stem Cell-Based Cartilage Tissue Engineering [Pubmed:25632940] |
| Osteoarthritis and Cartilage | Depletion of gangliosides enhances cartilage degradation in mice [Sciencedirect:S1063458413010364] |
| Biol Res | The role of MCP-1-CCR2 ligand-receptor axis in chondrocyte degradation and disease progress in knee osteoarthritis [PubMed: 26578310] |
| PLoS One | Interleukin-1 Receptor Antagonist Has a Novel Function in the Regulation of Matrix Metalloproteinase-13 Expression [PubMed: 26474296] |
| Cell Mol Immunol? | Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica [Pubmed:27157496] |
| Scientific Reports | Chondrogenic Potential of Peripheral Blood Derived Mesenchymal Stem Cells Seeded on Demineralized Cancellous Bone Scaffolds [pubmed:27821864] |
| International Journal of Contemporary Medical Research | Matrix Metalloproteinase-13 (MMP-13): A Novel Tumor Marker for Diagnosis of Breast Carcinoma [uploads:7] |
| Scientific Reports | Olive and grape seed extract prevents post-traumatic osteoarthritis damages and exhibits in vitro anti IL-1β activities before and after oral consumption. [pubmed:27640363] |
| Photodermatol Photoimmunol Photomed. | Protective effects of Aloe sterols against UVB‐induced photoaging in hairless mice [pubmed:27995657] |
| Molecular Medicine Reports | Icariin inhibits MMP?1, MMP?3 and MMP?13 expression through MAPK pathways in IL?1β?stimulated SW1353 chondrosarcoma cells [pubmed:28447732] |
| bmc musculoskeletal disorders | Strontium ranelate causes osteophytes overgrowth in a model of early phase osteoarthritis. [pubmed:28187731] |
| Journal of Cellular Physiology | Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Models [pubmed:27739057] |
| BMC Musculoskeletal Disorders | Establishment of rat ankle post-traumatic osteoarthritis model induced by malleolus fracture [pubmed:29149841] |
| Journal of Applied Animal Research | Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts:an in vitro approach to bovine digital dermatitis [10.108:09712119.2017.1335208] |
| Alexandria Journal of Medicine | L-Carnitine ameliorates knee lesions in mono-iodoacetate induced osteoarthritis in rats [10.1016:j.ajme.2016.03.002] |
| National Journal of Integrated Research in Medicine | Matrix Metalloproteinase-13 Can Be Used As an Independent Diagnostic and Prognostic Marker for Breast Carcinoma. [AN=123116960] |
| Molecular?Medicine?Reports | Effects of GGCX overexpression on anterior cruciate ligament transection-induced osteoarthritis in rabbits [Pubmed:29257344] |
| Journal of Applied Animal Research? | Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts: an approach to bovine digital dermatitis [10.1080:09712119.2017.1335208] |
| Osteoarthritis Cartilage | Coordinated existence of multiple gangliosides is required for cartilage metabolism [Pubmed: 30471358] |
| PLoS One | Co-culture of osteochondral explants and synovial membrane as in vitro model for osteoarthritis [Pubmed: 30939166] |
| Pathobiology | Suppressed Expression but Not Activity of Collagenases MMP-1 and MMP-13 in Human Renal Carcinoma [Pubmed: 31129674] |
| Bone | DAla2GIP antagonizes H2O2-induced chondrocyte apoptosis and inflammatory factor secretion [Pubmed: 31283994] |
| Food & Function | A dietary polysaccharide from Eucheuma cottonii downregulates proinflammatory cytokines and ameliorates osteoarthritis-associated cartilage degradation in obese?… [] |
| JOURNAL OF CELLULAR BIOCHEMISTRY | Teneligliptin inhibits IL‐1β‐induced degradation of extracellular matrix in human chondrocytes [Pubmed: 32162384] |
| Bone Research | Effects of tetrahedral framework nucleic acid/wogonin complexes on osteoarthritis [Pubmed: 32047705] |
| Scientific Reports | Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosis [Pubmed: 31980722] |
| Nutrients | Chondroprotective Properties of Human-Enriched Serum Following Polyphenol Extract Absorption: Results from an Exploratory Clinical Trial [Pubmed: 31888255] |
| Nature Communications | Vulnerability of progeroid smooth muscle cells to biomechanical forces is mediated by MMP13 [Pubmed: 32807790] |
| Molecular Medicine Reports | MicroRNA-1 promotes cartilage matrix synthesis and regulates chondrocyte differentiation via post-transcriptional suppression of Ihh expression [Pubmed: 32705199] |
| RESEARCH SQUARE | Experimental Observation of the Sequence of Tibial Plateau Chondrocyte and Matrix Degeneration in Spontaneous Osteoarthritis in Guinea Pigs [] |
| Hyaluronic Acid versus Alpha-2-Macroglobulin Intra-Articular Injections for Amelioration of Knee Posttraumatic Osteoarthritis: A Rat Model [] | |
| Loss of autophagy in tibial plateau chondrocytes causes increased apoptosis of chondrocytes in spontaneous osteoarthritis of guinea pigs [] | |
| Food Funct | Undenatured type II collagen prevents and treats the osteoarthritis and motor function degradation in T2DM patients and db/db mice [33890588] |
| Nutrients | Metabolic and Anti-Inflammatory Protective Properties of Human Enriched Serum Following Artichoke Leaf Extract Absorption: Results from an Innovative Ex Vivo?… [34444810] |
| Sci Rep | Liraglutide, a glucagon-like peptide 1 receptor agonist, exerts analgesic, anti-inflammatory and anti-degradative actions in osteoarthritis [Pubmed:35091584] |
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