欧美综合自拍亚洲综合网_欧美黑人巨大videos异族_可乐操中文字幕_亚洲成av人福利专区网站_国产强奸视频在播放_免费无码国产在线53_最近最新的免費中文字幕_抖音免费视频素材_亚洲精品熟女一二区_国产无套粉嫩白浆在线麻豆

基質(zhì)金屬蛋白酶3(MMP3)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Matrix Metalloproteinase 3 (MMP3) ,etc. by FLIA (Flow Luminescence Immunoassay)

SL1; STMY; STMY1; STR1; Progelatinase; Stromelysin 1; Transin-1

（注：單次混測多因子不超過8個指標 ）

  • 基質(zhì)金屬蛋白酶3(MMP3)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • 基質(zhì)金屬蛋白酶3(MMP3)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒用于檢測基質(zhì)金屬蛋白酶3(MMP3)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的基質(zhì)金屬蛋白酶3(MMP3)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 78-104 96
EDTA plasma(n=5) 95-102 99
heparin plasma(n=5) 94-102 97

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的基質(zhì)金屬蛋白酶3(MMP3)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中基質(zhì)金屬蛋白酶3(MMP3)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 88-105% 89-101% 84-102% 88-95%
EDTA plasma(n=5) 94-105% 85-94% 82-104% 87-94%
heparin plasma(n=5) 89-101% 93-105% 97-104% 82-96%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
    37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將基質(zhì)金屬蛋白酶3(MMP3)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的基質(zhì)金屬蛋白酶3(MMP3)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的基質(zhì)金屬蛋白酶3(MMP3)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的基質(zhì)金屬蛋白酶3(MMP3)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。

贈品

相關(guān)產(chǎn)品

編號 適用物種：Homo sapiens (Human，人) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
EPA101Hu62 基質(zhì)金屬蛋白酶3(MMP3)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA101Hu62 基質(zhì)金屬蛋白酶3(MMP3)活性蛋白 Cell?culture;?Activity?Assays.
RPA101Hu01 基質(zhì)金屬蛋白酶3(MMP3)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA101Hu02 基質(zhì)金屬蛋白酶3(MMP3)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA101Hu04 基質(zhì)金屬蛋白酶3(MMP3)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA101Hu03 基質(zhì)金屬蛋白酶3(MMP3)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA101Hu61 基質(zhì)金屬蛋白酶3(MMP3)活性蛋白 Cell?culture;?Activity?Assays.
RPA101Hu05 基質(zhì)金屬蛋白酶3(MMP3)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA101Hu02 基質(zhì)金屬蛋白酶3(MMP3)多克隆抗體 WB; IHC; ICC; IP.
PAA101Hu01 基質(zhì)金屬蛋白酶3(MMP3)多克隆抗體 WB; IHC; ICC; IP.
PAA101Hu05 基質(zhì)金屬蛋白酶3(MMP3)多克隆抗體 WB; IHC; ICC; IP.
PAA101Hu03 基質(zhì)金屬蛋白酶3(MMP3)多克隆抗體 WB; IHC; ICC; IP.
MAA101Hu22 基質(zhì)金屬蛋白酶3(MMP3)單克隆抗體 WB; IHC; ICC; IP.
MAA101Hu23 基質(zhì)金屬蛋白酶3(MMP3)單克隆抗體 WB; IHC; ICC; IP.
MAA101Hu21 基質(zhì)金屬蛋白酶3(MMP3)單克隆抗體 WB; IHC; ICC; IP.
LAA101Hu72 基質(zhì)金屬蛋白酶3(MMP3)單克隆抗體(生物素標記) WB; IHC; ICC.
SEA101Hu 基質(zhì)金屬蛋白酶3(MMP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA101Hu 基質(zhì)金屬蛋白酶3(MMP3)檢測試劑盒(化學(xué)發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA101Hu 基質(zhì)金屬蛋白酶3(MMP3)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
PSA101Hu01 基質(zhì)金屬蛋白酶3(MMP3)抗體對 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA101Hu01 基質(zhì)金屬蛋白酶3(MMP3)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志 參考文獻
Journal of Bone and Joint Surgery Experimental osteoarthritis induced by surgical realignment of the patella in BALB/c mice [PubMed: 21119180]
Infection and Immunity Bovine Respiratory Syncytial Virus and Histophilus somni Interaction at the Alveolar Barrier [Pubmed: 23649093]
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source]
China Pharmacy Influence of Tongfengtai Granules on Inflammatory Factor in Acute Gouty Arthritis Model Rats [Source]
Thromb Haemost. Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke [Pubmed:24671655]
Connective Tissue Research Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes [Pubmed:25333747]
physiological research The skin remodeling in type 1 diabetes and insulin resistance animal models [PubMed: 26047379]
Physiol Res The skin remodeling in type 1 diabetes and insulin resistance animal models. [PubMed: 26047379]
Cellular & Molecular Immunology Effect of ebosin on modulating interleukin-2&bgr;-induced inflammatory responses in rat fibroblast-like synoviocytes [PubMed: 25938977]
Biol Res The role of MCP-1-CCR2 ligand-receptor axis in chondrocyte degradation and disease progress in knee osteoarthritis [PubMed: 26578310]
J Orthop Res. Tetrandrine suppresses articular inflammatory response by inhibiting pro-inflammatory factors via NF-κB inactivation. [Pubmed:26748661]
Cellular?& Molecular Immunology Effect of ebosin on modulating interleukin-1β-induced inflammatory responses in rat fibroblast-like synoviocytes [pubmed:25938977]
Journal of Orthopaedic?Research Tetrandrine suppresses articular inflammatory response by inhibiting pro‐inflammatory factors via NF‐κB inactivation [pubmed:26748661]
Mediators?of?Inflammation Interleukin-29?Enhances?Synovial?Inflammation?and?Cartilage?Degradation?in?Osteoarthritis. [pubmed:27433031]
Molecular Medicine Reports Icariin inhibits MMP?1, MMP?3 and MMP?13 expression through MAPK pathways in IL?1β?stimulated SW1353 chondrosarcoma cells [pubmed:28447732]
der Christian-Albrechts-Universit?t zu Kiel Wirkung schonender Laserverfahren auf den Komplexaus retinalem Pigmentepithel, Bruch′scher Membranund Choroidea - m?gliche Therapieoption der frühen altersabh?ngigen Makuladegeneration [1133074014:34]
REDVET - Revista electrónica de Veterinaria Measurement of Metalloproteinase-3 levels in synovial fluid from horses as biomarker of joint disease [E-ISSN: 1695-7504]
REDVET - Revista electrónica de Veterinaria Measurement of Metalloproteinase-3 levels in synovial fluid from horses as biomarker of joint disease - Medición de los niveles de Metaloproteinasa-3 en liquido sinovial comobiomarcador de enfermedad articular [ISSN 1695-7504]
Molecular Pharmacology Dexamethasone Down-regulates Endothelin Receptors and Reduces Endothelin-induced Production of Matrix Metalloproteinases in Cultured Rat Astrocytes [10.1124/mol.116.107300]
Theranostics Targeting intracellular MMPs efficiently inhibits tumor metastasis and angiogenesis [Pubmed:29774078]
Chinese Journal of Integrative Medicine Electroacupuncture Delays Cartilage Degeneration by Modulating Nuclear Factor-κB Signaling Pathway [Pubmed: 30656598]
Effects of a hyperimmunized egg product on voluntary physical activity levels, serum inflammatory markers, and owner perception of joint pain of dogs with?… []
PLoS One Co-culture of osteochondral explants and synovial membrane as in vitro model for osteoarthritis [Pubmed: 30939166]
ACS Biomaterials Science & Engineering Fluorogenic Probe for Detecting Active Matrix Metalloproteinase-3 (MMP-3) in Plasma and Peripheral Blood Neutrophils to Indicate the Severity of Rheumatoid?… []
World Journal of Pediatrics Regulation of JAK/STAT signal pathway by miR-21 in the pathogenesis of juvenile idiopathic arthritis [Pubmed: 31641939]
International Journal of Molecular Sciences Matrix Metalloproteinase-3 is Key Effector of TNF-α-Induced Collagen Degradation in Skin [Pubmed: 31652545]
JOURNAL OF CELLULAR BIOCHEMISTRY Teneligliptin inhibits IL‐1β‐induced degradation of extracellular matrix in human chondrocytes [Pubmed: 32162384]
Bone Research Effects of tetrahedral framework nucleic acid/wogonin complexes on osteoarthritis [Pubmed: 32047705]
Scientific Reports Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosis [Pubmed: 31980722]
Alexandria Journal for Veterinary Sciences The Clinicopathological Picture of Some Postpartum Disorders in Barki Ewes. []
Identification of Polyphenolic Compounds and Hepatoprotective Activity of Artichoke (Cynara Scolymus L.) Edible Part Extracts in Rats []
Food Funct Undenatured type II collagen prevents and treats the osteoarthritis and motor function degradation in T2DM patients and db/db mice [33890588]
journal biomedicine translational research Peanut (Arachis hypogaea) Shells Extract and Apis dorsata Honey Reduce Matrix Metalloproteinase-3 in Monosodium Iodoacetate-Induced Osteoarthritic??- []
JOURNAL OF ANIMAL SCIENCE Physical Activity Patterns of Free Living Dogs Diagnosed with Osteoarthritis [34216471]
Osteoarthritis Cartilage A systematic review on the potential value of synovial fluid biomarkers to predict clinical outcomes in cartilage repair treatments [Pubmed:35618204]
Phytomedicine Transcriptomic profiling revealed the role of apigenin-4′-O-α-L-rhamnoside in inhibiting the activation of rheumatoid arthritis fibroblast-like synoviocytes via MAPK?… [Pubmed:35660352]
Journal of Immunology Research Ebosin Attenuates the Inflammatory Responses Induced by TNF-α through Inhibiting NF-κB and MAPK Pathways in Rat Fibroblast-Like Synoviocytes [Pubmed:35340587]
留言咨詢