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神經(jīng)肽Y(NPY)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Neuropeptide Y (NPY) ,etc. by FLIA (Flow Luminescence Immunoassay)

NP-Y; PYY4; CPON; Pro-neuropeptide Y; Neuropeptide tyrosine; C-flanking peptide of NPY

（注：單次混測多因子不超過8個指標 ）

  • 神經(jīng)肽Y(NPY)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • 神經(jīng)肽Y(NPY)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒用于檢測神經(jīng)肽Y(NPY)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的神經(jīng)肽Y(NPY)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 79-103 84
EDTA plasma(n=5) 79-101 84
heparin plasma(n=5) 82-93 86
sodium citrate plasma(n=5) 86-103 93

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的神經(jīng)肽Y(NPY)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中神經(jīng)肽Y(NPY)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 92-105% 94-101% 99-105% 78-102%
EDTA plasma(n=5) 83-97% 94-104% 79-90% 91-101%
heparin plasma(n=5) 79-101% 90-97% 88-99% 79-104%
sodium citrate plasma(n=5) 85-102% 99-105% 79-94% 92-99%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠、檢測溶液A）標準品或樣本50μL及磁珠10μL，加檢測溶液A50μL，
    37°C酶標板振蕩器孵育60分鐘；
3. 磁吸洗板3次；
4. 加檢測溶液B100μL，37°C振動孵育30分鐘；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將神經(jīng)肽Y(NPY)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠、標記VEGFA，標記的神經(jīng)肽Y(NPY)等多因子檢測試劑盒(流式熒光發(fā)光法)和未標記的神經(jīng)肽Y(NPY)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競爭結(jié)合，然后將未結(jié)合物洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的神經(jīng)肽Y(NPY)等多因子檢測試劑盒(流式熒光發(fā)光法)呈負相關(guān)。

贈品

相關(guān)產(chǎn)品

編號 適用物種：Cavia (Guinea pig ，豚鼠) 應用(僅供研究使用，不用于臨床診斷！)
CEA879Gu 神經(jīng)肽Y(NPY)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA879Gu 神經(jīng)肽Y(NPY)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻

雜志 參考文獻
Hemodialysis International Is there any interaction of resistin and adiponectin levels with protein‐energy wasting among patients with chronic kidney disease [Onlinelibrary: hdi.12072]
Journal of Pediatric Endocrinology and Metabolism Changes in growth pattern, leptin ghrelin and neuropeptide Y levels after adenotonsillectomy in prepubertal children [Degruyter: Source]
Ren Fail. The role of adipocytokines on depressive symptoms of patients with chronic kidney disease [Pubmed: 23879728]
Coronary Artery Disease The relationship of plasma neuropeptide Y levels with coronary collateral development. [Pubmed: 24077326]
Coronary Artery Disease The relationship of plasma neuropeptide Y levels with coronary collateral development [Lww:Source]
Toxicology Letters Possible role of serotonin and neuropeptide Y on the disruption of the reproductive axis activity by perfluorooctane sulfonate [Pubmed:25623392]
J Neurosurg Anesthesiol High Plasma Levels of Neuropeptide Y Correlate With Good Clinical Outcome But are not Correlated to Cerebral Blood Flow or Vasospasm After Subarachnoid Hemorrhage [PubMed: 25955865]
Journal of Diabetes Research High Intensity Aerobic Exercise Training Improves Deficits of Cardiovascular Autonomic Function in a Rat Model of Type 1 Diabetes Mellitus with Moderate … [Pubmed:26885531]
Archives of Gynecology and Obstetrics Binge eating and biochemical markers of appetite in new users of the contraceptive depot medroxyprogesterone acetate [pubmed:27604241]
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Hum Psychopharmacol Serum levels of neuropeptide Y in patients with chronic schizophrenia during treatment augmentation with sarcosine (results of the double?\blind randomized??- [33245168]
Arch Toxicol Serotonin involvement in okadaic acid-induced diarrhoea in vivo [34148100]
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