欧美综合自拍亚洲综合网_欧美黑人巨大videos异族_可乐操中文字幕_亚洲成av人福利专区网站_国产强奸视频在播放_免费无码国产在线53_最近最新的免費中文字幕_抖音免费视频素材_亚洲精品熟女一二区_国产无套粉嫩白浆在线麻豆

誘導(dǎo)型一氧化氮合酶(NOS2)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Nitric Oxide Synthase 2, Inducible (NOS2) ,etc. by FLIA (Flow Luminescence Immunoassay)

NOS2A; INOS; HEP-NOS; I-NOS; Hepatocytes Oxide Synthase; Peptidyl-cysteine S-nitrosylase NOS2

（注：單次混測多因子不超過8個指標(biāo) ）

  • 誘導(dǎo)型一氧化氮合酶(NOS2)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • 誘導(dǎo)型一氧化氮合酶(NOS2)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒用于檢測誘導(dǎo)型一氧化氮合酶(NOS2)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。

實驗流程

1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠）標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL，
    37°C酶標(biāo)板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標(biāo)板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將誘導(dǎo)型一氧化氮合酶(NOS2)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠，其中的誘導(dǎo)型一氧化氮合酶(NOS2)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的誘導(dǎo)型一氧化氮合酶(NOS2)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的誘導(dǎo)型一氧化氮合酶(NOS2)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。

贈品

相關(guān)產(chǎn)品

編號 適用物種：Rattus norvegicus (Rat，大鼠) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA837Ra02 誘導(dǎo)型一氧化氮合酶(NOS2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA837Ra01 誘導(dǎo)型一氧化氮合酶(NOS2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA837Ra02 誘導(dǎo)型一氧化氮合酶(NOS2)多克隆抗體 WB; IHC; ICC; IP.
PAA837Ra01 誘導(dǎo)型一氧化氮合酶(NOS2)多克隆抗體 WB; IHC; ICC; IP.
MAA837Ra21 誘導(dǎo)型一氧化氮合酶(NOS2)單克隆抗體 WB; IHC; ICC; IP.
SEA837Ra 誘導(dǎo)型一氧化氮合酶(NOS2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA837Ra 誘導(dǎo)型一氧化氮合酶(NOS2)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA837Ra01 誘導(dǎo)型一氧化氮合酶(NOS2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease High-molecular weight hyaluronan reduced renal PKC activation in genetically diabetic mice [PubMed: 20713153]
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease Hyaluronan reduces inflammation in experimental arthritis by modulating TLR-2 and TLR-4 cartilage expression [ScienceDirect: S0925443911001323]
FEBS Journal Adenosine A2a Receptor Activation and Hyaluronan Fragment Inhibition Reduce Inflammation in Mouse Articular Chondrocytes Stimulated with Il-1beta [Wiley: source]
Gene Hyaluronan in part mediates IL-1beta-induced inflammation in mouse chondrocytes by up-regulating CD44 receptors [ScienceDirect: S0378111911007347]
PLoS One A Novel Mechanism of Formaldehyde Neurotoxicity: Inhibition of Hydrogen Sulfide Generation by Promoting Overproduction of Nitric Oxide [PubMed: 23359814]
PLoS ONE Regulation of Microglia Activity by Glaucocalyxin-A: Attenuation of Lipopolysaccharide-Stimulated Neuroinflammation through NF-κB and p38 MAPK Signaling Pathways [PlosOne: Source]
Journal of Cellular Biochemistry The inhibition of hyaluronan degradation reduced pro-inflammatory cytokines in mouse synovial fibroblasts subjected to collagen-induced arthritis. [PubMed: 22234777]
International Immunopharmacology Amentoflavone inhibits iNOS, COX-2 expression and modulates cytokine profile, NF-κB signal transduction pathways in rats with ulcerative colitis [Pubmed: 24126114]
Integrative Cancer Therapies Decalepis hamiltonii inhibits tumor progression and metastasis by regulating the inflammatory mediators and nuclear factor κB subunits [Pubmed: 24013642]
Food and Chemical Toxicology Inhibition of UVB-induced skin phototoxicity by a grape seed extract as modulator of nitrosative stress, ERK/NF-kB signaling pathway and apoptosis, in SKH-1 mice [Pubmed: 23567245]
Experimental Gerontology Age-related changes in ADMA–DDAH–NO pathway in rat liver subjected to partial ischemia followed by global reperfusion [ScienceDirect: S0531556513003331]
International Immunopharmacology Protective effect of marine mangrove Rhizophora apiculata on acetic acid induced experimental colitis by regulating anti-oxidant enzymes, inflammatory mediators and nuclear factor-kappa B subunits [ScienceDirect: S1567576913004153]
INTERNATIONAL JOURNAL OF MEDICINE AND MEDICAL RESEARCH L-ARGININE, BUT NOT L-NAME PROTECTS AGAINST LIVER INJURY INDUCED BY EXPERIMENTAL ISCHEMIA-REPERFUSION [Tdmu:Source]
Molecular Vision Effects of topically applied tocotrienol on cataractogenesis and lens redox status in galactosemic rats [Pubmed:24940038]
Journal of Environmental Pathology, Toxicology and Oncology Protective Effect of?Acacia ferruginea?against Ulcerative Colitis via Modulating Inflammatory Mediators, Cytokine Profile and NF-κB Signal Transduction Pathways [Pubmed:24941292]
Eur J Cancer Prev. Utility of Apc-mutant rats with a colitis-associated colon carcinogenesis model for chemoprevention studies [Pubmed:25222193]
Cell and Tissue Research Beta-arrestin 1 is involved in the catabolic response stimulated by hyaluronan degradation in mouse chondrocytes [Pubmed:25673209]
Ann N Y Acad Sci.? Modulating effect of Biophytum sensitivum extract on rats with acetic acid-induced ulcerative colitis [Pubmed:5243883]
Molecular Medicine Reports Resveratrol ameliorates oxidative stress and inhibits aquaporin 4 expression following rat cerebral ischemia-reperfusion injury [PubMed: 26458999]
Chem Biol Interact Apocynin protects against ethanol-induced gastric ulcer in rats by attenuating the upregulation of NADPH oxidases 1 and 4 [PubMed: 26522475]
Journal of Environmental Pathology, Toxicology and Oncology Protective Effect of [PubMed: 26349607]
Journal of Immunotoxicology Acacia ferruginea inhibits inflammation by regulating inflammatory iNOS and COX-2 [PubMed: 25738525]
Cancer Medicine Circulating CD14+HLA-DR-/low myeloid-derived suppressor cells in leukemia patients with allogeneic hematopoietic stem cell transplantation: novel clinical potential strategies for the prevention and cellular therapy of graft-versus-host disease [Pubmed:27109254]
Journal of Natural Medicines Anti-oxidative, anti-secretory and anti-inflammatory activities of the extract from the root bark of Lycium chinense (Cortex Lycii) against gastric ulcer in mice [Pubmed:26972100]
Cell Mol Immunol? Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica [Pubmed:27157496]
Медицина и здравоохранение Роль индуцибельной NO-синтазы в механизмах регуляции функциональной активности кальций-активируемых калиевых каналов коронарных сосудов у крыс, адаптированных к стрессу [article:n]
Graefe's Archive for Clinical and Experimental Ophthalmology Role of RhoA/MERK1/ERK1/2/iNOS signaling in ocular ischemic syndrome [pubmed:27538909]
Taylor & Francis Influences of Hyriopsis cumingii polysaccharides on mice immunosignaling molecules and T lymphocyte differentiation [09540105.2017.1306494]
Molecular Vision Mechanism of the anticataract effect of liposomal MgT in galactose-fed rats. [pubmed:27440992]
Journal of Natural Medicines Anti-oxidative,?anti-secretory?and?anti-inflammatory?activities?of the?extract?from the?root?bark?ofLycium?chinense?(Cortex Lycii) against gastric ulcer in mice. [pubmed:26972100]
Applied?Biochemistry?and?Biotechnology Protective?Effect?of?Zingiber?officinale?Against?Dalton's?Lymphoma?Ascites?Tumour?byRegulating?Inflammatory?Mediator?and?Cytokines. [pubmed:27435276]
Cell Mol Neurobiol Posttraumatic Stress Disorder Disturbs Coronary Tone and Its Regulatory Mechanisms [pubmed:28676988]
Microbial Immunology Immunomodulatory Activities of a Fungal Protein Extracted from Hericium erinaceus through Regulating the Gut Microbiota [pubmed:28713364]
Biomedicine & Pharmacotherapy Protective effect of Averrhoa bilimbi L. fruit extract on ulcerative colitis in wistar rats via regulation of inflammatory mediators and cytokines [pubmed:28531922]
The Royal Society of Chemistry Anti-ulcerogenic effect of KFP-H008 against ethanol-induced gastric ulcer via p38 MAPK/NF-κB pathway [10.1039/C7RA08879E]
Acta Pharmacologica Sinica Cis-stilbene glucoside in Polygonum multiflorum induces immunological idiosyncratic hepatotoxicity in LPS-treated rats by suppressing PPAR-γ. [pubmed:28649126]
Cytokine Soy isoflavone genistein attenuates lipopolysaccharide-induced cognitive impairments in the rat via exerting anti-oxidative and anti-inflammatory effects [pubmed:29102164]
Molecules Melatonin balance the autophagy and apoptosis by regulating UCP2 in the LPS-induced cardiomyopathy [Pubmed:29547569]
European?Journal of Pharmacology? Naringenin ameliorates learning and memory impairment following systemic lipopolysaccharide challenge in the rat [Pubmed:29518393]
Regulatory Toxicology and Pharmacology Targeted inhibition of tumor survival, metastasis and angiogenesis by Acacia ferruginea mediated regulation of VEGF, inflammatory mediators, cytokine profile and?… [Pubmed:29678767]
Pharmacological Reports The Association between Inflammatory Markers (iNOS, HO-1, IL-33, MIP-1β) and Depression with and without Posttraumatic Stress Disorder [10.1016:j.pharep.2018.06.001]
Clinical?And Experimental Pharmacology And Physiology? Diallyl sulfide alleviates cisplatin‐induced nephrotoxicity in rats via suppressing NF‐κB downstream inflammatory proteins and p53/Puma signalling pathway [Pubmed:29266336]
St??enia ?ródb?onkowej i indukowalnej formy syntazy tlenku azotu w surowicy krwi kobiet ci??arnych pal?cych tytoń–badania wst?pne []
Journal of?Allergy?and?Clinical?Immunology Exosome swarms eliminate airway pathogens and provide passive epithelial immunoprotection through nitric oxide [Pubmed: 30442371]
Metabolic?Brain?Disease Trigonelline protects hippocampus against intracerebral Aβ (1–40) as a model of Alzheimer's disease in the rat: insights into underlying mechanisms [Pubmed: 30421246]
PLoS?One Intraphagolysosomal conditions predispose to Staphylococcus epidermidis small colony variants persistence in macrophages [Pubmed: 30412620]
The?Eurasian?Journal of Medicine Tadalafil Preserves Penile Nitric Oxide Synthase from Detrimental Effect of Paroxetine in Rats [Doi: 10.5152/eurasianjmed.2018.18160]
PLoS One Macrophage expression of E3 ubiquitin ligase Grail protects mice from lipopolysaccharide-induced hyperinflammation and organ injury [Pubmed: 30571701]
International Journal of Environmental Research and?Public?Health Influence of Active Exposure to Tobacco Smoke on Nitric Oxide Status of Pregnant Women [Pubmed: 30513890]
Journal of Functional Foods Complex interaction of dietary fat and Alaskan bog blueberry supplementation influences manganese mediated neurotoxicity and behavioral impairments []
Particle and?Fibre?Toxicology Silica nanoparticles trigger the vascular endothelial dysfunction and prethrombotic state via miR-451 directly regulating the IL6R signaling pathway [Pubmed: 30975181]
ВЛИЯНИЕ ИНДУЦИБЕЛЬНОЙ NO-СИНТАЗЫ НА ТОНУС КОРОНАРНЫХ СОСУДОВ, СОКРАТИТЕЛЬНУЮ ФУНКЦИЮ МИОКАРДА И МЕХАНИЗМЫ?… []
Journal of Cosmetic Dermatology Whitening effects of cosmetic formulation in the vascular component of skin pigmentation [Pubmed: 31074159]
regional hemodynamics and microcirculation Benzimidazole Derivative, 2-ETH Prevents Poststressor Disorders of Coronary Vascular Tone and Myocardial Contractility []
Life Sci Cilostazol mitigates mesenteric ischemia/reperfusion-induced lung lesion: Contribution of PPAR-|?, NF-|êB, and STAT3 crosstalk [33310046]
PLoS One Correlation analysis of the proportion of monocytic myeloid-derived suppressor cells in colorectal cancer patients [33370317]
Biotechnol Rep Targeting NF-|êB mediated cell signaling pathway and inflammatory mediators by 1, 2-diazole in A549 cells in vitro [33598414]
Basic and Clinical Neuroscience Paeonol Protects Against Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson's Disease [33995926]
Fermented Foods and Microbes Related to Health Cordyceps cicadae NTTU 868 Mycelium with The Addition of Bioavailable Forms of Magnesium from Deep Ocean Water Prevents the A|?40 and Streptozotocin??- []
Ya?lanma ve Hipertansiyonda Yeni bir Endoltelyal Disfonksiyon Belirteci: ??z¨1nebilir Lektin Benzeri Okside D¨1?¨1k Dansiteli Lipoprotein-1 []
Fermentation Cordyceps cicadae NTTU 868 Mycelium with The Addition of Bioavailable Forms of Magnesium from Deep Ocean Water Prevents the A|?40 and Streptozotocin-Induced Memory Deficit via Suppressing Alzheimer?ˉs Disease Risk Factors and Increasing Magnesium Uptake of Brain []
Drug Chem Toxicol Study of cardioprotective activity of the methanolic extract of the aerial parts of Bauhinia madagascariensis compared to Bauhinia purpurea against adrenaline?… [34167392]
Frontiers in Cardiovascular Medicine The Effects of Intermittent Hypoxic–Hyperoxic Exposures on Lipid Profile and Inflammation in Patients With Metabolic Syndrome [34513946]
Nutrients Erinacine A-Enriched Hericium erinaceus Mycelium Delays Progression of Age-Related Cognitive Decline in Senescence Accelerated Mouse Prone 8 (SAMP8) Mice [34684662]
biomolecules Whole-Body Cryotherapy Increases the Activity of Nitric Oxide Synthase in Older Men [34356664]
Hum Exp Toxicol Carvacrol hinders the progression of hepatic fibrosis via targeting autotaxin and thioredoxin in thioacetamide-induced liver fibrosis in rat [34155936]
J Ethnopharmacol Post-stroke treatment of storax improves long-term outcomes of stroke in rats [34343648]
Phytomedicine A multidimensional strategy for uncovering comprehensive quality markers of Schisandra chinensis (Turcz.) Baill based on pharmacodynamics and chemical?… []
留言咨詢