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血小板因子4(PF4)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Platelet Factor 4 (PF4) ,etc. by FLIA (Flow Luminescence Immunoassay)

CXCL4; SCYB4; Chemokine C-X-C-Motif Ligand 4; Oncostatin-A; Iroplact

（注：單次混測多因子不超過8個(gè)指標(biāo) ）

  • 血小板因子4(PF4)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • 血小板因子4(PF4)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒用于檢測血小板因子4(PF4)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的血小板因子4(PF4)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 86-95 90
EDTA plasma(n=5) 93-101 96
heparin plasma(n=5) 87-101 92
sodium citrate plasma(n=5) 94-103 98

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的血小板因子4(PF4)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中血小板因子4(PF4)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 95-103% 80-89% 78-94% 88-99%
EDTA plasma(n=5) 78-97% 84-99% 98-105% 87-98%
heparin plasma(n=5) 85-92% 83-92% 96-104% 78-96%
sodium citrate plasma(n=5) 78-97% 95-102% 84-95% 96-105%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。

實(shí)驗(yàn)流程

1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠）標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL，
    37°C酶標(biāo)板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標(biāo)板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動(dòng)孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實(shí)驗(yàn)原理

將血小板因子4(PF4)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠，其中的血小板因子4(PF4)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的血小板因子4(PF4)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的血小板因子4(PF4)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。

贈(zèng)品

相關(guān)產(chǎn)品

編號 適用物種：Mus musculus (Mouse，小鼠) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
APA172Mu01 血小板因子4(PF4)活性蛋白 Cell?culture;?Activity?Assays.
RPA172Mu01 血小板因子4(PF4)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA172Mu02 血小板因子4(PF4)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA172Mu01 血小板因子4(PF4)多克隆抗體 WB; IHC; ICC; IP.
PAA172Mu02 血小板因子4(PF4)多克隆抗體 WB; IHC; ICC; IP.
LAA172Mu71 血小板因子4(PF4)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
LAA172Mu81 血小板因子4(PF4)多克隆抗體(異硫氰酸熒光素標(biāo)記) WB; IHC; ICC; IF.
SEA172Mu 血小板因子4(PF4)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA172Mu 血小板因子4(PF4)檢測試劑盒(化學(xué)發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA172Mu 血小板因子4(PF4)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA172Mu01 血小板因子4(PF4)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics Propofol lowers serum PF4 level and partially corrects hypercoagulopathy in endotoxemic rats [PubMed: 20601223]
Basic Research in Cardiology Secretome of apoptotic peripheral blood cells (APOSEC) attenuates microvascular obstruction in a porcine closed chest reperfused acute myocardial infarction model: role of platelet aggregation and vasodilation [PubMed: 22899170]
PLoS One Platelets Recognize Brain-Specific Glycolipid Structures, Respond to Neurovascular Damage and Promote Neuroinflammation [PubMed: PMC3608633]
Diving and Hyperbaric Medicine Effect of splenectomy on platelet activation and decompression sickness outcome in a rat model of decompression [Pubmed:25311322]
Journal of Functional Foods A?Phellinus baumii–based supplement containing?Salvia miltiorrhiza?Bunge improves atherothrombotic profiles through endothelial nitric oxide synthase and cyclooxygenase pathwaysin vitro?and?in vivo [science:S1756464616300676]
Plos one Aspirin, but Not Tirofiban Displays Protective Effects in Endotoxin Induced Lung Injury [pubmed:27583400]
international journal of molecular sciences Evidence of the Role of R-Spondin 1 and Its Receptor Lgr4 in the Transmission of Mechanical Stimuli to Biological Signals for Bone Formation. [pubmed:28272338]
Experimental?and?Therapeutic?Medicine Feasibility of improving platelet?rich plasma therapy by using chitosan with high platelet activation ability [pubmed:28450960]
Reproductive Toxicology Aspirin pre-treatment modulates ozone-induced fetal growth restriction and alterations in uterine blood flow in rats [Pubmed: 30528429]
INTERNATIONAL JOURNAL OF CARDIOLOGY Short and long-term changes in platelet and inflammatory biomarkers after cryoballoon and radiofrequency ablation [Pubmed: 30857843]
Available at SSRN 3335365? Evaluating the Platelet Activation Related to the Degradation of Biomaterials by Scheme of Molecular Markers []
journal of biotechnology Newly Identified HNP‐F from Human Neutrophil Peptide 1 Promotes Hemostasis [Pubmed: 30927490]
Data in Brief Data for short and long-term prothrombotic biomarkers after cryoballoon and radiofrequency ablation []
journal of thoracic and cardiovascular surgery Evaluating Platelet Activation Related to the Degradation of Biomaterials Using Molecular Markers [Pubmed: 32812629]
Marine Drugs The Inhibitory Effect of Protamine on Platelets is Attenuated by Heparin without Inducing Thrombocytopenia in Rodents [Pubmed: 31533230]
Thrombosis Research Feasibility study of use of rabbit blood to evaluate platelet activation by medical devices [Pubmed: 31838449]
Nat Commun The C5a/C5a receptor 1 axis controls tissue neovascularization through CXCL4 release from platelets [34099640]
Vaccine The effect of ChAdOx1 nCov-19 vaccine on arterial thrombosis development and platelet aggregation in female rats [Pubmed:35183388]
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