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催乳素(PRL)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Prolactin (PRL) ,etc. by FLIA (Flow Luminescence Immunoassay)

LTH; Luteotropic Hormone

（注：單次混測多因子不超過8個指標 ）

  • 催乳素(PRL)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • 催乳素(PRL)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒用于檢測催乳素(PRL)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的催乳素(PRL)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 80-102 89
EDTA plasma(n=5) 84-103 101
heparin plasma(n=5) 97-105 101
sodium citrate plasma(n=5) 91-98 94

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的催乳素(PRL)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中催乳素(PRL)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 85-97% 83-94% 97-104% 82-103%
EDTA plasma(n=5) 93-102% 78-98% 96-104% 83-101%
heparin plasma(n=5) 79-90% 78-89% 94-101% 81-89%
sodium citrate plasma(n=5) 85-92% 82-102% 81-94% 85-104%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
    37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將催乳素(PRL)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的催乳素(PRL)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的催乳素(PRL)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的催乳素(PRL)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關。

贈品

相關產(chǎn)品

編號 適用物種：Bos taurus; Bovine (Cattle，牛) 應用(僅供研究使用，不用于臨床診斷！)
APA846Bo01 催乳素(PRL)活性蛋白 Cell?culture;?Activity?Assays.
RPA846Bo01 催乳素(PRL)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA846Bo01 催乳素(PRL)多克隆抗體 WB; IHC; ICC; IP.
MAA846Bo21 催乳素(PRL)單克隆抗體 WB; IHC; ICC; IP.
SEA846Bo 催乳素(PRL)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA846Bo 催乳素(PRL)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA846Bo 催乳素(PRL)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA846Bo01 催乳素(PRL)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) Main materials for "Do It(ELISA Kit) Yourself"

參考文獻

雜志 參考文獻
The Journal of Biological Chemistry In Vivo Evidence for Epidermal Growth Factor Receptor (EGFR)-mediated Release of Prolactin from the Pituitary Gland [Jcb: 39297]
Journal of Clinical Neuroscience TLR9 expression is associated with prognosis in patients with glioblastoma multiforme [ScienceDirect: S0967586811003572]
Sensors and Actuators B: Chemical Determination of prolactin hormone in serum and urine using an electrochemical immunosensor based on poly(pyrrolepropionic acid)/carbon nanotubes hybrid modified electrodes [Sciencedirect:S0925400514000720]
Veterinary Word Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifers [Pubmed:27051212]
Journal?of?Dairy?Science Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calves [publication:306322503]
Stem Cell Research & Therapy Differentiation of human umbilical cord Wharton’s jelly-derived mesenchymal stem cells into endometrial cells [pubmed:29096715]
Stem?Cell?Research?&?Therapy The role of mesenchymal stem cells in chemotherapy-induced gonadotoxicity [Pubmed:30021657]
Frontiers in Molecular Neuroscience Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice [Pubmed:29403355]
Toxicology and?Applied?Pharmacology The antipsychotics sulpiride induces fatty liver in rats via phosphorylation of insulin receptor substrate-1 at Serine 307-mediated adipose tissue insulin resistance [Pubmed:29551354]
Journal of Cellular Biochemistry The prolactin‐release inhibitor paeoniflorin suppresses proliferation and induces apoptosis in prolactinoma cells via the mitochondria‐dependent pathway [Pubmed:29388711]
Journal?of?Cellular?and?Molecular?Medicine Metformin inhibits growth and prolactin secretion of pituitary prolactinoma cells and xenografts [Pubmed: 30334324]
BMC?Veterinary Research Hormonal and metabolic indicators before and after farrowing in sows affected with postpartum dysgalactia syndrome [Pubmed: 30404636]
International Journal of Basic and Clinical Endocrinology Expression of prolactin receptors in the duodenum, kidneys and skeletal system during physiological and sulpiride-induced hyperprolactinaemia [Pubmed: 30143940]
MORPHOLOGY AND PATHOMORPHOLOGY Morphological and Biochemical Characteristics of Prostate Hyperplasia during Sulpiride Treatment [Pubmed: 32152847]
JOURNAL OF DAIRY SCIENCE Effect of heat stress during the early and late dry period on mammary gland development of Holstein dairy cattle [Pubmed: 32684470]
BioMed Research International Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress [Pubmed: 32685488]
Vet Microbiol Prolactin affects the disappearance of ALV-J viremia in vivo and inhibits viral infection [34391195]
Antioxidants (Basel) Effects of a Nanoencapsulated Moringa Leaf Ethanolic Extract on the Physiology, Metabolism and Reproductive Performance of Rabbit Does during Summer [34439574]
Nutrients Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome Mice [Pubmed:35057459]
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