核因子κB受體激活因子配體(RANkL)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) ,etc. by FLIA (Flow Luminescence Immunoassay)
CD254; TNFSF11; ODF; OPGL; TRANCE; HRANKL2; SOdf; Tumor Necrosis Factor(ligand)superfamily Member 11; TNF-related activation-induced cytokine
（注：單次混測多因子不超過8個(gè)指標(biāo) ）
- 編號LMA855Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長3.5h
- 檢測范圍0.98-1000pg/mL
- 靈敏度最小可檢測劑量小于等于0.327 pg/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 下載 英文說明書 中文說明書
- 規(guī)格 8指標(biāo)數(shù) 7指標(biāo)數(shù) 6指標(biāo)數(shù) 5指標(biāo)數(shù) 4指標(biāo)數(shù) 3指標(biāo)數(shù) 2指標(biāo)數(shù)1指標(biāo)數(shù)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測核因子κB受體激活因子配體(RANkL)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的核因子κB受體激活因子配體(RANkL)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 81-99 | 92 |
| EDTA plasma(n=5) | 80-95 | 90 |
| heparin plasma(n=5) | 83-90 | 87 |
| sodium citrate plasma(n=5) | 91-98 | 94 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的核因子κB受體激活因子配體(RANkL)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中核因子κB受體激活因子配體(RANkL)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 85-98% | 96-105% | 91-101% | 80-99% |
| EDTA plasma(n=5) | 79-101% | 80-97% | 89-97% | 93-101% |
| heparin plasma(n=5) | 97-105% | 91-103% | 80-97% | 80-102% |
| sodium citrate plasma(n=5) | 85-92% | 97-104% | 98-105% | 91-98% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠）標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL，
37°C酶標(biāo)板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標(biāo)板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動(dòng)孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實(shí)驗(yàn)原理
將核因子κB受體激活因子配體(RANkL)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠，其中的核因子κB受體激活因子配體(RANkL)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的核因子κB受體激活因子配體(RANkL)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的核因子κB受體激活因子配體(RANkL)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。
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相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APA855Ra61 | 核因子κB受體激活因子配體(RANkL)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA855Ra01 | 核因子κB受體激活因子配體(RANkL)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA855Ra01 | 核因子κB受體激活因子配體(RANkL)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA855Ra24 | 核因子κB受體激活因子配體(RANkL)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA855Ra26 | 核因子κB受體激活因子配體(RANkL)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA855Ra22 | 核因子κB受體激活因子配體(RANkL)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA855Ra23 | 核因子κB受體激活因子配體(RANkL)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA855Ra21 | 核因子κB受體激活因子配體(RANkL)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA855Ra25 | 核因子κB受體激活因子配體(RANkL)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA855Ra27 | 核因子κB受體激活因子配體(RANkL)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA855Ra28 | 核因子κB受體激活因子配體(RANkL)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA855Ra29 | 核因子κB受體激活因子配體(RANkL)單克隆抗體 | WB; IHC; ICC; IP. |
| LAA855Ra76 | 核因子κB受體激活因子配體(RANkL)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| SEA855Ra | 核因子κB受體激活因子配體(RANkL)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA855Ra | 核因子κB受體激活因子配體(RANkL)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA855Ra | 核因子κB受體激活因子配體(RANkL)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| PSA855Ra01 | 核因子κB受體激活因子配體(RANkL)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA855Ra01 | 核因子κB受體激活因子配體(RANkL)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
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