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硒蛋白P1(SEPP1)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Selenoprotein P1, Plasma (SEPP1) ,etc. by FLIA (Flow Luminescence Immunoassay)

SEPP; SeP; SEP-P1; SELP

（注：單次混測多因子不超過8個指標 ）

  • 硒蛋白P1(SEPP1)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • 硒蛋白P1(SEPP1)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒用于檢測硒蛋白P1(SEPP1)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的硒蛋白P1(SEPP1)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 86-99 95
EDTA plasma(n=5) 82-101 90
heparin plasma(n=5) 93-101 96

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的硒蛋白P1(SEPP1)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中硒蛋白P1(SEPP1)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 93-101% 98-105% 91-99% 97-104%
EDTA plasma(n=5) 97-104% 88-95% 92-101% 78-105%
heparin plasma(n=5) 92-101% 88-101% 79-101% 99-105%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
    37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將硒蛋白P1(SEPP1)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的硒蛋白P1(SEPP1)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的硒蛋白P1(SEPP1)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的硒蛋白P1(SEPP1)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。

贈品

相關(guān)產(chǎn)品

編號 適用物種：Rattus norvegicus (Rat，大鼠) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPB809Ra01 硒蛋白P1(SEPP1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB809Ra01 硒蛋白P1(SEPP1)多克隆抗體 WB; IHC; ICC; IP.
MAB809Ra21 硒蛋白P1(SEPP1)單克隆抗體 WB; IHC; ICC; IP.
SEB809Ra 硒蛋白P1(SEPP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB809Ra 硒蛋白P1(SEPP1)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻

雜志 參考文獻
The Journal of Clinical Endocrinology & Metabolism Serum Selenoprotein P Levels in Patients with Type 2 Diabetes and Prediabetes: Implications for Insulin Resistance, Inflammation, and Atherosclerosis [PubMed: 21677040]
Diabetes & metabolism journal Increased Selenoprotein P Levels in Subjects with Visceral Obesity and Nonalcoholic Fatty Liver Disease [PubMed: 23439771]
Journal of Pediatric Biochemistry Trace elements, heavy metals and vitamins in Egyptian school children with iron deficiency anemia [Metapress:Source]
Journal of Trace Elements in Medicine and Biology Selenium and its relationship with selenoprotein P and glutathione peroxidase in children and adolescents with Hashimoto&#039;s thyroiditis and hypothyroidism [PubMed: 26854239]
OBES RES CLIN PRACT/OBESITY RESEARCH & CLINICAL PRACTICE Selenoprotein?P?is?elevated?in?individuals?with?obesity, but is not?independently?associated?withinsulin?resistance. [pubmed:27524654]
Annals of Nutrition and Metabolism Acute?Overfeeding?Does Not Alter Liver or?Adipose?Tissue-Derived?Cytokines?in?HealthyHumans. [pubmed:27832637]
European?Journal?of?Nutrition DNA?damage?and?oxidative?stress?response?to?selenium?yeast?in the?non-smoking?individuals: ashort-term?supplementation?trial?with?respect?to?GPX1?and SEPP1 polymorphism. [pubmed:26658762]
South. Clin. Ist. Euras. Evaluation of the Relationship Between Insulin Resistance and Selenoprotein P in Patients with Polycystic Ovary Syndrome [DOI: 10.14744/scie.2017.87597]
Arch Endocrinol Metab Serum selenium and selenoprotein-P levels in autoimmune thyroid dieases patients in a select center a transversal study [DOI: 10.1590/2359-3997000000309]
British Journal of Nutrition Selenium, selenoproteins and selenometabolites in mothers and babies at the time of birth [pubmed:28534447]
Journal of?Trace?Elements?in Medicine and Biology Biomarkers of selenium status and antioxidant effect in workers occupationally exposed to mercury [Pubmed:29895371]
Biological &?Pharmceutical Bulletin Comparison of Human Selenoprotein P Determinants in Serum between Our Original Methods and Commercially Available Kits [Pubmed:29709922]
Diabetologia Non-alcoholic fatty liver disease in the first trimester and subsequent development of gestational diabetes mellitus [Pubmed: 30470912]
Biological Trace Element Research Selenium Levels in Community Dwellers with Type 2 Diabetes Mellitus [Pubmed: 30725267]
Advances in?Medical?Sciences Can hepatokines be regarded as novel non-invasive serum biomarkers of intrahepatic lipid content in obese children? [Pubmed: 30921653]
Acute swimming exercise, but not exposure to moderate hypoxic conditions reduces circulating selenoprotein P levels in short-term, high-fat diet-fed rats [Pubmed: 31236416]
Hormones Selenium and selenoprotein P in nonalcoholic fatty liver disease [Pubmed: 31493247]
Bull Environ Contam Toxicol Soil Selenium Concentration and Residents Daily Dietary Intake in a Selenosis Area: A Preliminary Study in Yutangba Village, Enshi City, China [Pubmed: 32909074]
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Higher Serum Selenoprotein P Level as a Novel Inductor of Metabolic Complications in Psoriasis [Pubmed: 32605214]
Circulating hepassocin level in patients with stable angina is associated with fatty liver and renal function []
Nat Immunol Selenium–GPX4 axis protects follicular helper T cells from ferroptosis [34413521]
Reviews in Cardiovascular Medicine Selenoprotein P-1 (SEPP1) as An Early Biomarker of Acute Kidney Injury in Patients Undergoing Cardiopulmonary Bypass []
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