生長(zhǎng)抑素(SST)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Somatostatin (SST) ,etc. by FLIA (Flow Luminescence Immunoassay)
GHIH; SRIF; SMST; GHRIH; GHRIF; Growth Hormone Releasing Inhibiting Hormone; Somatotropin Release-Inhibiting Factor; Somatostatin-14; Somatostatin-28
(注:?jiǎn)未位鞙y(cè)多因子不超過8個(gè)指標(biāo) )
- 編號(hào)LMA592Mu
- 物種Mus musculus (Mouse,小鼠) 相同的名稱,不同的物種。
- 實(shí)驗(yàn)方法競(jìng)爭(zhēng)抑制
- 反應(yīng)時(shí)長(zhǎng)1.5h
- 檢測(cè)范圍0.49-500pg/mL
- 靈敏度最小可檢測(cè)劑量小于等于0.163 pg/mL.
- 樣本類型serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 8指標(biāo)數(shù) 7指標(biāo)數(shù) 6指標(biāo)數(shù) 5指標(biāo)數(shù) 4指標(biāo)數(shù) 3指標(biāo)數(shù) 2指標(biāo)數(shù)1指標(biāo)數(shù)
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特異性
本試劑盒用于檢測(cè)生長(zhǎng)抑素(SST)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法),經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制,不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè),因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的生長(zhǎng)抑素(SST)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)(加標(biāo)樣品),重復(fù)測(cè)定并計(jì)算其均值,回收率為測(cè)定值與理論值的比率。
樣本 | 回收率范圍(%) | 平均回收率(%) |
serum(n=5) | 96-105 | 101 |
EDTA plasma(n=5) | 82-102 | 96 |
heparin plasma(n=5) | 80-94 | 80 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次,分別計(jì)算不同濃度樣本的平均值及SD值。
批間差:選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定,每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次,分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的生長(zhǎng)抑素(SST)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法),并倍比稀釋成1:2,1:4,1:8,1:16的待測(cè)樣本,線性范圍即為稀釋后樣本中生長(zhǎng)抑素(SST)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)含量的測(cè)定值與理論值的比率。
樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 92-101% | 80-90% | 83-95% | 98-105% |
EDTA plasma(n=5) | 81-98% | 78-103% | 89-96% | 78-89% |
heparin plasma(n=5) | 85-98% | 97-104% | 91-98% | 84-103% |
穩(wěn)定性
經(jīng)測(cè)定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響,實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致,尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備;
2. 加樣(標(biāo)準(zhǔn)品、樣本、磁珠、檢測(cè)溶液A)標(biāo)準(zhǔn)品或樣本50μL及磁珠10μL,加檢測(cè)溶液A50μL,
37°C酶標(biāo)板振蕩器孵育60分鐘;
3. 磁吸洗板3次;
4. 加檢測(cè)溶液B100μL,37°C振動(dòng)孵育30分鐘;
5. 磁吸洗板3次;
6. 加鞘液100μL,旋渦震蕩2分鐘后讀數(shù)。
實(shí)驗(yàn)原理
將生長(zhǎng)抑素(SST)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)抗體包被于磁珠,制成固相載體,向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠、標(biāo)記VEGFA,標(biāo)記的生長(zhǎng)抑素(SST)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)和未標(biāo)記的生長(zhǎng)抑素(SST)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競(jìng)爭(zhēng)結(jié)合,然后將未結(jié)合物洗凈后,加入PE標(biāo)記的親和素,再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的生長(zhǎng)抑素(SST)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)呈負(fù)相關(guān)。
贈(zèng)品
相關(guān)產(chǎn)品
編號(hào) | 適用物種:Mus musculus (Mouse,小鼠) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPA592Mu01 | 生長(zhǎng)抑素(SST)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA592Mu02 | 生長(zhǎng)抑素(SST)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
CPA592Mu11 | 生長(zhǎng)抑素(SST)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
CPA592Mu21 | 生長(zhǎng)抑素(SST)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
PAA592Mu01 | 生長(zhǎng)抑素(SST)多克隆抗體 | WB; IHC; ICC; IP. |
PAA592Mu02 | 生長(zhǎng)抑素(SST)多克隆抗體 | WB; IHC; ICC; IP. |
PAA592Mu08 | 生長(zhǎng)抑素(SST)多克隆抗體 | WB; IHC; ICC; IP. |
LAA592Mu71 | 生長(zhǎng)抑素(SST)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
MAA592Mu22 | 生長(zhǎng)抑素(SST)單克隆抗體 | WB; IHC; ICC; IP. |
MAA592Mu23 | 生長(zhǎng)抑素(SST)單克隆抗體 | WB; IHC; ICC; IP. |
MAA592Mu24 | 生長(zhǎng)抑素(SST)單克隆抗體 | WB; IHC; ICC; IP. |
MAA592Mu25 | 生長(zhǎng)抑素(SST)單克隆抗體 | WB; IHC; ICC; IP. |
MAA592Mu21 | 生長(zhǎng)抑素(SST)單克隆抗體 | WB; IHC; ICC; IP. |
LAA592Mu72 | 生長(zhǎng)抑素(SST)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
SEA592Mu | 生長(zhǎng)抑素(SST)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CEA592Mu | 生長(zhǎng)抑素(SST)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA592Mu | 生長(zhǎng)抑素(SST)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
PSA592Mu01 | 生長(zhǎng)抑素(SST)抗體對(duì) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSA592Mu11 | 生長(zhǎng)抑素(SST)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
JOP. Journal of the Pancreas | Pancreatic Somatostatinoma Diagnosed Preoperatively: Report of a Case [Pubmed: 24413789] |
PloS one | Circadian Modulation of Anxiety: A Role for Somatostatin in the Amygdala [Pubmed: 24376834] |
Peptides | Enhanced expression of neuropeptide S (NPS) receptor in eosinophils from severe asthmatics and subjects with total IgE above 100IU/ml [Pubmed:24239856] |
Journal of Endocrinology | Inhibitory effect of somatostatin on insulin secretion is not mediated via the CNS [Pubmed:25630331] |
Pharmacol Res | Oral curcumin has anti-arthritic efficacy through somatostatin generation [PubMed: 25836921] |
Acta?Neurobiologiae Experimentalis | Aberrant changes of somatostatin and neuropeptide Y in brain of a genetic rat model for epilepsy: tremor rat [pubmed:27685769] |
Applied Biological Chemistry | Positive enhancement of Lactobacillus fermentum HY01 on intestinal movements of mice having constipation [10.1007/s13765-017-0327-3] |
Biomed Research International | Colonic Transit Time and Gut Peptides in Adult Patients with Slow and Normal Colonic Transit Constipation [pubmed:29082242] |
Molecular Neurobiology | Somatostatin Maintains Permeability and Integrity of Blood-Brain Barrier in β-Amyloid Induced Toxicity [Pubmed:29700775] |
Evidence-Based?Complementary and Alternative Medicine | Home-Based Transcutaneous Neuromodulation Improved Constipation via Modulating Gastrointestinal Hormones and Bile Acids [Pubmed:29853946] |
Pancreatology | Relationship between somatostatin and interleukin-6: A cross-sectional study in patients with acute pancreatitis [Pubmed: 30279074] |
Cell?Reports | Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in Mice [Pubmed: 30404008] |
Science?of?Food?and?Agriculture | Protective Effect of Silkworm Pupa Oil on Hydrochloric Acid/Ethanol‐Induced Gastric Ulcer [Pubmed: 30479041] |