血管內(nèi)皮生長因子A(VEGFA)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Vascular Endothelial Growth Factor A (VEGFA) ,etc. by FLIA (Flow Luminescence Immunoassay)
VEGF; VEGF-A; VPF; Vascular Endothelial Cell Growth Factor A; Vascular Permeability Factor
（注：單次混測多因子不超過8個指標(biāo) ）
- 編號LMA143Mi
- 物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時長3.5h
- 檢測范圍0.98-1000pg/mL
- 靈敏度最小可檢測劑量小于等于0.327 pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 8指標(biāo)數(shù) 7指標(biāo)數(shù) 6指標(biāo)數(shù) 5指標(biāo)數(shù) 4指標(biāo)數(shù) 3指標(biāo)數(shù) 2指標(biāo)數(shù)1指標(biāo)數(shù)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒對檢測血管內(nèi)皮生長因子A(VEGFA)等多因子檢測試劑盒(流式熒光發(fā)光法)具有較高的靈敏度。
經(jīng)檢測小鼠、大鼠之間有100%的交叉反映。
回收率
分別于定值血清及血漿樣本中加入一定量的血管內(nèi)皮生長因子A(VEGFA)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 95-103 | 99 |
| EDTA plasma(n=5) | 96-105 | 101 |
| heparin plasma(n=5) | 80-97 | 81 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的血管內(nèi)皮生長因子A(VEGFA)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中血管內(nèi)皮生長因子A(VEGFA)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-92% | 87-95% | 83-97% | 90-99% |
| EDTA plasma(n=5) | 94-103% | 89-98% | 96-104% | 96-103% |
| heparin plasma(n=5) | 80-94% | 79-90% | 83-102% | 92-102% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠）標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL，
37°C酶標(biāo)板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標(biāo)板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實(shí)驗(yàn)原理
將血管內(nèi)皮生長因子A(VEGFA)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠，其中的血管內(nèi)皮生長因子A(VEGFA)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的血管內(nèi)皮生長因子A(VEGFA)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的血管內(nèi)皮生長因子A(VEGFA)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| SEA143Mi | 血管內(nèi)皮生長因子A(VEGFA)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA143Mi | 血管內(nèi)皮生長因子A(VEGFA)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
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| Menoufia Medical Journal | Study of serum level of vascular endothelial growth factor in patients with hepatocellular carcinoma [] |
| Acta Scientific Women's Health | Modulatory Effects of Taurine Combined with Letrozole on Ehrlich Ascites Carcinoma in Mice [] |
| Indian Journal of Public Health Research & Development | Enhanced Fibrin-Lysis in Grade-1 Dengue Haemorrhagic Fever [] |
| Materials Science and Engineering: C | Cell-modified bioprinted microspheres for vascular regeneration [] |
| Strawberry (Fragaria x ananassa) leaf extract reduces brain damage in diabetic male rats [] | |
| Stem Cell Reviews and Reports | Prospects on the Potential In Vitro Regenerative Features of Mechanically Treated-Adipose Tissue for Osteoarthritis Care [33469783] |
| Advanced Healthcare Materials | Accelerated Bone Regeneration by MOF Modified Multifunctional Membranes through Enhancement of Osteogenic and Angiogenic Performance [33448103] |
| Aesthet Surg J | Adipose-Derived Stem Cells Improve the Aging Skin of Nude Mice by Promoting Angiogenesis and Reducing Local Tissue Water [33428732] |
| INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | In-vivo evaluation of alginate-pectin hydrogel film loaded with simvastatin for diabetic wound healing in Streptozotocin-induced diabetic rats [33421467] |
| Eur J Pharmacol | Lactucaxanthin protects retinal pigment epithelium from hyperglycemia-regulated hypoxia/ER stress/VEGF pathway mediated angiogenesis in ARPE-19 cell and rat ?- [33705802] |
| Acta Biomater | Highly interconnected inverse opal extracellular matrix scaffolds enhance stem cell therapy in limb ischemia [33878473] |
| Journal of Tissue Engineering | Therapeutic potential of adipose-derived mesenchymal stem cell exosomes in tissue-engineered bladders [33868627] |
| EXPERIMENTAL EYE RESEARCH | Astaxanthin mediated regulation of VEGF through HIF1|á and XBP1 signaling pathway: An insight from ARPE-19 cell and streptozotocin mediated diabetic rat model [33789142] |
| Randomized Controlled Trial | Effect of Freeze-Dried Amnion Graft on Cytokines in Uterine Exudates Following Hysteroscopic Adhesiolysis of Severe Intrauterine Adhesions [28170094] |
| cancer communications | Programmed death ligand?\1 regulates angiogenesis and metastasis by participating in the c?\JUN/VEGFR2 signaling axis in ovarian cancer [33939321] |
| ?D1ú??éú3??§ó???éú3?2??ó?? | VEGFA/VEGFR2 ?úD?êó???à·????òòê×é?ˉ?a1üéú3é?Dμ?±í′??°×÷ó? [] |
| Chinese Journal of Parasitology and parasitic diseases | Expression and angiogenic effect of VEGFA/VEGFR2 in mice hepatic metacestode tissue of Echinococcus multilocularis [] |
| International Journal of Life science and Pharma Research | Artificial Sweeteners Alter Early Vitelline Vessel Development in Chick Embryo.(2021) [] |
| Biomed Mater | Preclinical efficacy study of a porous biopolymeric scaffold based on (gelatin-hyaluronic acid-chondroitin sulfate) in a porcine burn injury model: Role of critical?… [34384056] |
| J Cell Mol Med | Mesenchymal stem cells from different sources show distinct therapeutic effects in hyperoxia‐induced bronchopulmonary dysplasia in rats [34322990] |
| J Biochem Mol Toxicol | Thymoquinone upregulates miR‐125a‐5p, attenuates STAT3 activation, and potentiates doxorubicin antitumor activity in murine solid Ehrlich carcinoma [34605108] |
| Bioengineered | MicroRNA-934 facilitates cell proliferation, migration, invasion and angiogenesis in colorectal cancer by targeting B-cell translocation gene 2: Running title: miR934?… [34699325] |
| ACS Biomater Sci Eng | Polydopamine-Coated Poly(l-lactide) Nanofibers with Controlled Release of VEGF and BMP-2 as a Regenerative Periosteum [34472855] |
| Cells | A-Kinase Anchoring Protein 2 Promotes Protection against Myocardial Infarction [34831084] |
| J Basic Clin Physiol Pharmacol | Anti-atherosclerotic activity of aqueous extract of Ipomoea batatas (L.) leaves in high-fat diet-induced atherosclerosis model rats [Pubmed:34986543] |
| Stem Cell Res Ther | A dual role of HIF1α in regulating osteogenesis–angiogenesis coupling [Pubmed:35123567] |
| American Journal of Translational Research | Curcumin treats endometriosis in mice by the HIF signaling pathway [Pubmed:35559378] |
| Clinical and Experimental Immunology | Clinical significance of differential serum-signatures for early prediction of severe dengue among Eastern Indian patients [Pubmed:35348620] |
| Medicina | Assessment of the RANTES Level Correlation and Selected Inflammatory and Pro-Angiogenic Molecules Evaluation of Their Influence on CRC Clinical Features: A?… [Pubmed:35208526] |
| Molecular Biology Reports | Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling?… [Pubmed:35585377] |
| Biomaterials Advances | Human adipose-derived stem cell-loaded small intestinal submucosa as a bioactive wound dressing for the treatment of diabetic wounds in rats [] |
| Biointerface Research in Applied Chemistry | Beetroot Improves the Therapeutic Efficacy of Bone Marrow-Derived Mesenchymal Stem Cells in Preventing Cisplatin Induced Nephrotoxicity in Male Rats [] |
| Research Square | DMOG attenuates steroid-associated endothelial progenitor cell impairment and osteonecrosis of the femoral head via regulating HIF-1α signaling pathway in?… [] |
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