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內脂素(VF)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Visfatin (VF) ,etc. by FLIA (Flow Luminescence Immunoassay)

Nampt; NAmPRTase; PBEF; PBEF1; Pre-B-Cell Colony Enhancing Factor; Nicotinamide Phosphoribosyltransferase

（注：單次混測多因子不超過8個指標 ）

  • 內脂素(VF)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • 內脂素(VF)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

本試劑盒用于檢測內脂素(VF)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的內脂素(VF)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 95-102 99
EDTA plasma(n=5) 91-99 96
heparin plasma(n=5) 80-99 91

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內加入適量的內脂素(VF)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中內脂素(VF)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 82-103% 93-101% 94-103% 86-102%
EDTA plasma(n=5) 94-102% 93-101% 98-105% 79-105%
heparin plasma(n=5) 95-103% 81-101% 82-101% 93-102%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
    37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將內脂素(VF)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的內脂素(VF)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結合，然后加入生物素化的內脂素(VF)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的內脂素(VF)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關。

贈品

相關產(chǎn)品

編號 適用物種：Bos taurus; Bovine (Cattle，牛) 應用(僅供研究使用，不用于臨床診斷！)
RPA638Bo01 內脂素(VF)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA638Bo01 內脂素(VF)多克隆抗體 WB; IHC; ICC; IP.
MAA638Bo21 內脂素(VF)單克隆抗體 WB; IHC; ICC; IP.
SEA638Bo 內脂素(VF)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA638Bo 內脂素(VF)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻

雜志 參考文獻
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J Physiol Pharmacol. Early-effect of bariatric surgery (Scopinaro method) on intestinal hormones and adipokines in insulin resistant Wistar rat. [Pubmed: 24304571]
Endocr J. CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes [Pubmed:25168658]
Exp Ther Med. Plasma visfatin levels and mRNA expression of visfatin in peripheral blood mononuclear cells and peripheral blood monocyte-derived macrophages from normal weight females with polycystic ovary syndrome [Pubmed:Pmc3991522]
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