堿性成纖維細胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)
Mini Samples ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)
B-FGF; BFGF; FGFB; HBGH-2; Basic Fibroblast Growth Factor; Heparin-binding growth factor 2
- 編號MEA551Mu
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應(yīng)時長3h
- 檢測范圍12.35-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于4.55pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 3268 ¥ 4668 ¥ 21006 ¥ 39678 ¥ 326760
- 欲了解實際交易價格和更多情況，請與當?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
實驗結(jié)果圖
-
標準曲線圖
-
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測堿性成纖維細胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的堿性成纖維細胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)（加標樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 88-103 | 92 |
| EDTA plasma(n=5) | 87-96 | 91 |
| heparin plasma(n=5) | 96-104 | 101 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的堿性成纖維細胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中堿性成纖維細胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 82-89% | 90-103% | 83-92% | 93-102% |
| EDTA plasma(n=5) | 79-101% | 78-104% | 88-95% | 86-96% |
| heparin plasma(n=5) | 83-102% | 88-102% | 92-105% | 83-101% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）25µL后，立即加入檢測溶液A25µL，
37°C孵育1小時；
3. 甩干，洗板3次；
4. 加檢測溶液B50µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物50µL，37°C孵育10－20分鐘；
7. 加終止液25µL，立即450nm讀數(shù)。
實驗原理
本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測定標本中待測物質(zhì)水平。將堿性成纖維細胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入生物素標記的抗原和待測抗原(標準品或樣本)，待測抗原與生物素標記抗原對特異性抗體進行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測標本濃度越高，標記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測物質(zhì)含量呈負相關(guān)。用酶標儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APA551Mu01 | 堿性成纖維細胞生長因子(FGF2)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA551Mu02 | 堿性成纖維細胞生長因子(FGF2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA551Mu01 | 堿性成纖維細胞生長因子(FGF2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA551Mu01 | 堿性成纖維細胞生長因子(FGF2)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA551Mu02 | 堿性成纖維細胞生長因子(FGF2)多克隆抗體 | WB; IHC; ICC; IP. |
| SEA551Mu | 堿性成纖維細胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA551Mu | 堿性成纖維細胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA551Mu | 堿性成纖維細胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA551Mu | 堿性成纖維細胞生長因子(FGF2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA551Mu11 | 堿性成纖維細胞生長因子(FGF2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Journal of Pediatric Surgery | The influence of nutrients, biliary-pancreatic secretions, and systemic trophic hormones on intestinal adaptation in a Roux-en-Y bypass model [PubMed: 20438940] |
| International Journal of Nanomedicine | Effect of heparan sulfate and gold nanoparticles on muscle development during embryogenesis [PubMed: PMC3254262] |
| Archives of Animal Nutrition | Effect of taurine and gold nanoparticles on the morphological and molecular characteristics of muscle development during chicken embryogenesis [Tandfonline: 644918] |
| Diabetes | Peroxisome Proliferator–Activated Receptor-γ Coactivator-1α (PGC-1α) Enhances Engraftment and Angiogenesis of Mesenchymal Stem Cells in Diabetic Hindlimb Ischemia [Pubmed: 22266669] |
| Molecular Pharmacology | Electrospun Fibers with Plasmid bFGF Polyplex Loadings Promote Skin Wound Healing in Diabetic Rats [Pubmed: 22091745] |
| Science Alert | Antiangiogenic Activities of Cinnamon, Black and Green Tea Extracts on Experimentally Induced Breast Cancer in Rats [Scialert: Source] |
| Nanoscale Research Letters | Silver nanoparticles administered to chicken affect VEGFA and FGF2 gene expression in breast muscle and heart. [PubMed: 22827927] |
| PLoS One | Carbon nanoparticles downregulate expression of basic fibroblast growth factor in the heart during embryogenesis [PubMed: PMC3771850] |
| International Journal of Molecular Sciences | Nano-Nutrition of Chicken Embryos. The Effect of in Ovo Administration of Diamond Nanoparticles and L-Glutamine on Molecular Responses in Chicken Embryo Pectoral Muscles [NCBI:PMC3856104] |
| Archives of Animal Nutrition | Nano-nutrition of chicken embryos. The effect of silver nanoparticles and ATP on expression of chosen genes involved in myogenesis [Pubmed: 23952606] |
| Journal of Rehabilitation Research & Development | Role of sensory and motor intensity of electrical stimulation on fibroblastic growth factor-2 expression, inflammation, vascularization, and mechanical strength of full-thickness wounds [Pubmed: 23934870] |
| PLoS One.? | Carboxypeptidase E-ΔN, a Neuroprotein Transiently Expressed during Development Protects Embryonic Neurons against Glutamate Neurotoxicity [Pubmed:25426952] |
| Implant Dentistry | Effects of Hemostatic Agents on Fibroblast Cells [Lww:Source] |
| Journal of Materials Chemistry B | bFGF-grafted electrospun fibrous scaffolds via poly(dopamine) for skin wound healing [Rsc:Source] |
| Archives of Animal Nutrition | Effect of silver nanoparticles and hydroxyproline, administered in ovo, on the development of blood vessels and cartilage collagen structure in chicken embryos [Pubmed:25530495] |
| Int J Clin Exp Med. | The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cell [PubMed: 26628972] |
| Theriogenology | Evaluation of Changes in Doppler Ultrasonography Indices and Levels of Maternal Serum Angiogenic Factors throughout Pregnancy in Ewes [Science: Article] |
| Tumor Biology | Hypoxia-induced secretion of IL-10 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma [PubMed: 26695151] |
| Acta Biomaterialia | Preparation and characterization of pro-angiogenic gel derived from small intestinal submucosa [article:S1742706115301410] |
| 22 | Surface biofunctional drug-loaded electrospun fibrous scaffolds for comprehensive repairing hypertrophic scars [Pubmed:26774564] |
| Genet Mol Res | Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells [Pubmed:27323064] |
| BMC?Neuroscience? | 3-O-Methyldopa inhibits astrocyte-mediated dopaminergic neuroprotective effects of l-DOPA [pubmed:27456338] |
| Medical Science Monitor | 5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts [898221] |
| Biomedicine & Pharmacotherapy | FGF-2-mediated FGFR1 signaling in human microvascular endothelial cells is activated by vaccarin to promote angiogenesis [pubmed:28841454] |
| Medical Science Monitor | 5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts [pubmed:28052053] |
| International Journal of?Clinical?and Experimental Pathology | Platelet-derived growth factor and stromal cell-derived factor-1 promote the skin wound repairing effect of bone mesenchymal stem cells: a key role of matrix metalloproteinase 1 and collagens [ISSN:1936-2625/IJCEP0058424] |
| Journal of Biomaterials and Tissue Engineering | The Combination of Acellular Porcine Small Intestinal Submucosa (SIS) Cryogel Granules and Adipose Tissue Improves the Survival Ratio of Fat Grafting in Mice [10.1166/jbt.2017.1652] |
| Stem?Cells?and Development | Lower Senescence of Adipose-Derived Stem Cells than Donor-Matched Bone Marrow Stem Cells for Surgical Ventricular Restoration [Pubmed:29630447] |
| Neuroscience?Letters | Increased serum FGF2 levels in first-episode, drug-free patients with schizophrenia [Pubmed: 30172685] |
| Biochemistry and?Cell?Biology | Silencing of Astrocyte elevated gene-1 (AEG-1) inhibits proliferation, migration and invasion, and promotes apoptosis in pancreatic cancer cells [Pubmed: 30359541] |
| Journal of Materials Chemistry B | Gene-activating skin substitute comprised of PLLA/POSS nanofibers and plasmid DNAs encoding ANG and bFGF promotes in vivo revascularization and?… [] |
| BMC?Ophthalmology | HOXA9 is a novel myopia risk gene [Pubmed: 30674274] |
| Applied Materials Today | Immunomodulated electrospun fibrous scaffolds via bFGF camouflage for pelvic regeneration [] |
| International Journal of Ophthalmology | A novel xeno-free culture system for human retinal pigment epithelium cells [Pubmed: 31024807] |
| Open Access Macedonian Journal of Medical Sciences | Low Degree Hyaluronic Acid Crosslinking Inducing the Release of TGF-Β1 in Conditioned Medium of Wharton's Jelly-Derived Stem Cells [] |
| Chemico-Biological Interactions | Bisphenol S triggers the malignancy of hemangioma cells via regulation of basic fibroblast growth factor [Pubmed: 31669319] |
| International Journal of Molecular Sciences | β-Galactosylceramidase Deficiency Causes Bone Marrow Vascular Defects in an Animal Model of Krabbe Disease [Pubmed: 31905906] |
| Molecular Medicine Reports | Long non?coding RNA NORAD regulates angiogenesis of human umbilical vein endothelial cells via miR?590?3p under hypoxic conditions [Pubmed: 32323787] |
| Basic Fibroblast Growth Factor is Involved in Bisphenol S Induced Proliferation of Hemangioma Cells [] | |
| Bioact Mater | New design to remove leukocytes from platelet-rich plasma (PRP) based on cell dimension rather than density [33842739] |
| Acta Biomater | Highly interconnected inverse opal extracellular matrix scaffolds enhance stem cell therapy in limb ischemia [33878473] |
| Materials Science & Engineering C | Double Layer Composite Membrane for Preventing Tendon Adhesion and Promoting Tendon Healing [33812576] |
| Ratlarda deneysel kolon anastomoz ka?a?? modelindeintraperitoneal atorvastatin uygulamas?n?n anastomozka?a?? onar?m? ¨1zerine etkisi [] | |
| World J Hepatol | Leukocyte cell-derived chemotaxin-2 and fibroblast growth factor 21 in alcohol-induced liver cirrhosis [Pubmed:35070009] |
| Biomaterials Advances | Human adipose-derived stem cell-loaded small intestinal submucosa as a bioactive wound dressing for the treatment of diabetic wounds in rats [] |
留言咨詢
