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神經(jīng)肽Y(NPY)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)

Mini Samples ELISA Kit for Neuropeptide Y (NPY)

NP-Y; PYY4; CPON; Pro-neuropeptide Y; Neuropeptide tyrosine; C-flanking peptide of NPY

  • 神經(jīng)肽Y(NPY)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) 產(chǎn)品包裝(模擬)
  • 神經(jīng)肽Y(NPY)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) 產(chǎn)品包裝(模擬)
  • 神經(jīng)肽Y(NPY)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) 實驗結果圖
  • MEA879Hu.jpg 標準曲線圖
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒用于檢測神經(jīng)肽Y(NPY)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的神經(jīng)肽Y(NPY)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 99-105 102
EDTA plasma(n=5) 92-105 97
heparin plasma(n=5) 91-105 97

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的神經(jīng)肽Y(NPY)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中神經(jīng)肽Y(NPY)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 90-103% 82-105% 78-89% 85-103%
EDTA plasma(n=5) 78-91% 88-99% 82-99% 84-94%
heparin plasma(n=5) 92-99% 90-102% 92-101% 78-93%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）25µL后，立即加入檢測溶液A25µL，
    37°C孵育1小時；
3. 甩干，洗板3次；
4. 加檢測溶液B50µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物50µL，37°C孵育10－20分鐘；
7. 加終止液25µL，立即450nm讀數(shù)。

實驗原理

本試劑盒應用競爭抑制酶聯(lián)免疫分析法測定標本中待測物質(zhì)水平。將神經(jīng)肽Y(NPY)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入生物素標記的抗原和待測抗原(標準品或樣本)，待測抗原與生物素標記抗原對特異性抗體進行競爭結合。溫育后經(jīng)洗滌去掉未結合物，然后加入HRP標記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測標本濃度越高，標記抗原和抗體的結合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關，而與樣品中待測物質(zhì)含量呈負相關。用酶標儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。

相關產(chǎn)品

編號 適用物種：Homo sapiens (Human，人) 應用(僅供研究使用，不用于臨床診斷！)
RPA879Hu01 神經(jīng)肽Y(NPY)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
CPA879Hu22 神經(jīng)肽Y(NPY)卵白蛋白偶聯(lián)物 Immunogen; SDS-PAGE; WB.
CPA879Hu21 神經(jīng)肽Y(NPY)卵白蛋白偶聯(lián)物 Immunogen; SDS-PAGE; WB.
PAA879Hu08 神經(jīng)肽Y(NPY)多克隆抗體 WB; IHC; ICC; IP.
PAA879Hu01 神經(jīng)肽Y(NPY)多克隆抗體 WB; IHC; ICC; IP.
LAA879Hu71 神經(jīng)肽Y(NPY)多克隆抗體(生物素標記) WB; IHC; ICC.
MAA879Hu22 神經(jīng)肽Y(NPY)單克隆抗體 WB; IHC; ICC; IP.
MEA879Hu 神經(jīng)肽Y(NPY)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA879Hu 神經(jīng)肽Y(NPY)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA879Hu 神經(jīng)肽Y(NPY)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA879Hu11 神經(jīng)肽Y(NPY)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志 參考文獻
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Coronary Artery Disease The relationship of plasma neuropeptide Y levels with coronary collateral development. [Pubmed: 24077326]
Coronary Artery Disease The relationship of plasma neuropeptide Y levels with coronary collateral development [Lww:Source]
Toxicology Letters Possible role of serotonin and neuropeptide Y on the disruption of the reproductive axis activity by perfluorooctane sulfonate [Pubmed:25623392]
J Neurosurg Anesthesiol High Plasma Levels of Neuropeptide Y Correlate With Good Clinical Outcome But are not Correlated to Cerebral Blood Flow or Vasospasm After Subarachnoid Hemorrhage [PubMed: 25955865]
Journal of Diabetes Research High Intensity Aerobic Exercise Training Improves Deficits of Cardiovascular Autonomic Function in a Rat Model of Type 1 Diabetes Mellitus with Moderate … [Pubmed:26885531]
Archives of Gynecology and Obstetrics Binge eating and biochemical markers of appetite in new users of the contraceptive depot medroxyprogesterone acetate [pubmed:27604241]
basic & clinical pharmacology & toxicology Effects of Di-isononyl Phthalate on Neuropeptide Y Expression in Differentiating Human Neuronal Cells. [pubmed:27625336]
Acta?Neurobiologiae Experimentalis Aberrant changes of somatostatin and neuropeptide Y in brain of a genetic rat model for epilepsy: tremor rat [pubmed:27685769]
Cardiovascular?research Nur77 protects against adverse cardiac remodelling by limiting Neuropeptide Y signalling in the sympathoadrenal-cardiac axis [Pubmed:29850786]
Journal of Periodontology Salivary neuropeptides, stress, and periodontitis [Pubmed:28914594]
Neuropeptides Mechanisms of sustained long-term weight loss after RYGB: α-MSH is a key factor [Pubmed:29685637]
Pharmacology Melatonin Induces Osteoblastic Differentiation of Mesenchymal Stem Cells and Promotes Fracture Healing in a Rat Model of Femoral Fracture via Neuropeptide Y?… [Pubmed: 30227410]
Marmara Medical Journal Investigation of the relationship between insulin resistance and neuropeptide Y levels in polycystic ovary syndrome []
Marmara Medical Journal Investigation of the relationship between insulin resistance and neuropeptide Y levels in polycystic ovary syndrome. []
neurochemical research Sevoflurane Induces Hippocampal Neuronal Apoptosis by Altering the Level of Neuropeptide Y in Neonatal Rats. [Pubmed: 32378074]
Hum Psychopharmacol Serum levels of neuropeptide Y in patients with chronic schizophrenia during treatment augmentation with sarcosine (results of the double?\blind randomized??- [33245168]
Arch Toxicol Serotonin involvement in okadaic acid-induced diarrhoea in vivo [34148100]
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