載脂蛋白B(APOB)單克隆抗體
Monoclonal Antibody to Apolipoprotein B (APOB)
Apo-B; FLDB; Apo B-100; Apo B-48
- 編號(hào)MAC003Ra21
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(jià)(克隆號(hào))C3
- Ig亞型 IgG2a Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPC003Ra01-載脂蛋白B(APOB)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度1mg/ml
- 且適物種Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 720 ¥ 1680 ¥ 2400 ¥ 6000 ¥ 24000
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Rat Liver Tissue; Primary Ab: 30μg/ml Mouse Anti-Rat APOB Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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IHC圖
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DAB staining on IHC-P;
Sample: Rat Liver Tissue
Primary Ab: 30μg/ml Mouse Anti-Rat APOB Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Rat Skeletal muscle Tissue
Primary Ab: 30μg/ml Mouse Anti-Rat APOB Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
FITC staining on IF;
Samples: Human HepG2 cell;
Primary Ab: 20μg/ml Mouse Anti-Rat APOB Antibody
Second Ab: 1μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu18) -
FITC staining on IF;
Sample: HepG2 cell
Primary Ab: 20μg/ml Mouse Anti-Rat APOB Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)
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Figure. Western Blot; Sample: Recombinant APOB, Rat.
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Western Blot; Sample: Rat Liver lysate
Primary Ab: 5μg/ml Mouse Anti-Rat APOB Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) Selected -
Western Blot; Sample: HepG2 cell lysate
Primary Ab: 5μg/ml Mouse Anti-Rat APOB Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) Selected -
Western Blot; Sample: Porcine Liver lysate
Primary Ab: 5μg/ml Mouse Anti-Rat APOB Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) Selected
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)APOB的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別APOB。
用法
Western blotting: 0.01-5μg/mL;
Immunohistochemistry: 5-50μg/mL;
Immunocytochemistry: 5-50μg/mL;
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPC003Ra02 | 載脂蛋白B(APOB)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC003Ra01 | 載脂蛋白B(APOB)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC003Ra02 | 載脂蛋白B(APOB)多克隆抗體 | WB; IHC; ICC; IP. |
| PAC003Ra01 | 載脂蛋白B(APOB)多克隆抗體 | WB,IHC |
| LAC003Ra81 | 載脂蛋白B(APOB)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAC003Ra71 | 載脂蛋白B(APOB)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAC003Ra21 | 載脂蛋白B(APOB)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC003Ra23 | 載脂蛋白B(APOB)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC003Ra22 | 載脂蛋白B(APOB)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC003Ra24 | 載脂蛋白B(APOB)單克隆抗體 | WB; IHC; ICC; IP. |
| LAC003Ra72 | 載脂蛋白B(APOB)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| SEC003Ra | 載脂蛋白B(APOB)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC003Ra | 載脂蛋白B(APOB)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| PSC003Ra01 | 載脂蛋白B(APOB)抗體對(duì) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSC003Ra01 | 載脂蛋白B(APOB)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Lipid Research | Group 1B phospholipase A2 deficiency protects against diet-induced hyperlipidemia in mice [Jlr: M019463] |
| J Clin Endocrinol Metab.? | Elevated retinol binding protein 4 induces apolipoprotein B production and associates with hypertriglyceridemia [Pubmed:25781360] |
| Biochim Biophys Acta | Culturing of HepG2 cells with human serum improve their functionality and suitability in studies of lipid metabolism [PubMed: 26515253] |
| Am J Cardiovasc Dis | Hypoascorbemia induces atherosclerosis and vascular deposition of lipoprotein (a) in transgenic mice [PubMed: 26064792] |
| The Journal of Biological Chemistry | Proprotein convertase subtilisin/Kexin type 9 (PCSK9) single domain antibodies are potent inhibitors of LDL receptor degradation [Pubmed:27284008] |
| United States Patent Application | COMPOSITIONS AND METHODS FOR EFFICACIOUS AND SAFE DELIVERY OF SIRNA USING SPECIFIC CHITOSAN-BASED NANOCOMPLEXES [y2016:0152988.html] |
| alcohol and alcoholism | Hops (Humulus lupulus) Content in Beer Modulates Effects of Beer on the Liver After Acute Ingestion in Female Mice. [pubmed:27659607] |
| Frontiers in Physiology | Change in the Lipid Transport Capacity of the Liver and Blood during Reproduction in Rats [pubmed:28798692] |
| Food?Research International | Wild Lonicera caerulea berry polyphenol extract reduces cholesterol accumulation and enhances antioxidant capacity in vitro and in vivo [Pubmed:29580541] |
| Journal of The Science of Food and Agriculture | Consumption of orange fermented beverage improves antioxidant status and reduces peroxidation lipid and inflammatory markers in healthy humans [Pubmed:29124773] |
| European?Journal of Pharmacology | The effect of exenatide (a GLP-1 analog) and sitagliptin (a DPP-4 inhibitor) on plasma platelet-activating factor acetylhydrolase (PAF-AH) activity and concentration in?… [Pubmed: 30768981] |
| Nature Communications | Hepatic HuR modulates lipid homeostasis in response to high-fat diet [Pubmed: 32546794] |
| Study of in Vivo Serum Lipid Regulation with Ulmus macrocarpa Hance Extract in Rats [] | |
| Nat Commun | GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity [34853313] |
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