內(nèi)皮素1(EDN1)單克隆抗體
Monoclonal Antibody to Endothelin 1 (EDN1)
ET1; PPET1; Preproendothelin-1; Big endothelin-1
- 編號MAA482Hu23
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C4
- Ig亞型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 -
- 緩沖液成份磷酸鹽緩沖液(pH7.4,含有0.02% NaN3和50%甘油)
- 性狀液體
- 濃度1mg/mL
- 且適物種-
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù),請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 614 ¥ 1432 ¥ 2046 ¥ 5115 ¥ 20460
- 欲了解實(shí)際交易價格和更多情況,請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
特異性
該抗體是針對EDN1的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識別EDN1。
用法
Western blotting: 0.2-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲,損失率低于5%。
贈品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽性對照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長臂生物素標(biāo)記試劑盒
實(shí)時熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPA482Hu01 | 內(nèi)皮素1(EDN1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
CPA482Hu11 | 內(nèi)皮素1(EDN1)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
CPA482Hu21 | 內(nèi)皮素1(EDN1)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
PAA482Hu08 | 內(nèi)皮素1(EDN1)多克隆抗體 | WB; IHC; ICC; IP. |
PAA482Hu01 | 內(nèi)皮素1(EDN1)多克隆抗體 | WB; IHC; ICC; IP. |
LAA482Hu81 | 內(nèi)皮素1(EDN1)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
LAA482Hu71 | 內(nèi)皮素1(EDN1)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
MAA482Hu23 | 內(nèi)皮素1(EDN1)單克隆抗體 | WB; IHC; ICC; IP. |
MAA482Hu22 | 內(nèi)皮素1(EDN1)單克隆抗體 | WB; IHC; ICC; IP. |
MAA482Hu21 | 內(nèi)皮素1(EDN1)單克隆抗體 | WB; IHC; ICC; IP. |
LAA482Hu72 | 內(nèi)皮素1(EDN1)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
CEA482Hu | 內(nèi)皮素1(EDN1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CCA482Hu | 內(nèi)皮素1(EDN1)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMA482Hu | 內(nèi)皮素1(EDN1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSA482Hu11 | 內(nèi)皮素1(EDN1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Heart and Circulatory Physiology | B-type natriuretic peptide, vascular endothelial growth factor, endothelin-1, and nitric oxide synthase in chronic mountain sickness [APS: source] |
PLOS ONE | CD98 Positive Eosinophils Contribute to T Helper 1 Pattern Inflammation [PlosOne: Source] |
36 | Megakaryocytic leukemia 1 (MKL1) ties the epigenetic machinery to hypoxia-induced transactivation of endothelin-1 [PubMed: PMC3695508] |
Clinics (Sao Paulo). | Antifibrotic effects of crocetin in scleroderma fibroblasts and in bleomycin-induced sclerotic mice [Pubmed: 24212843] |
Life Sci. | Attenuation of renal ischemia/reperfusion injury by a?aí extract preconditioning in a rat model [Pubmed:25476829] |
Transpl Int. | One or 4 h of “in‐house” reconditioning by machine perfusion after cold storage improve reperfusion parameters in porcine kidneys [Pubmed:24977654] |
J Endocrinol Invest.? | The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disorders [Pubmed:25245337] |
Chemical Research in Toxicology | Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal Instillation [Pubmed:25580880] |
Journal of Surgical Research | Pivotal role of mast cell carboxypeptidase A in mediating protection against small intestinal ischemia–reperfusion injury in rats after ischemic preconditioning [Sciencedirect:S0022480414004909] |
Pharmaceutical Biology | The promising effect of barberry (Zereshk) extract against experimental pulmonary microvascular remodeling and hypertension: A comparison with sildenafil [PubMed: 26023989] |
A Periodical of the Hungarian Academy of Sciences | The effect of high altitude on endothelial and vascular dysfunction markers in preeclamptic patients [PubMed: 26690031] |
Chemical Research in Toxicology | Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal Instillation [PubMed: 25580880] |
Human & Experimental Toxicology | An in vivo and in vitro study High-dosage Danshen injection induces peripheral vascular endothelial cells injury [PubMed: 26078283] |
Artery Research | Endothelin 1 and endothelial dysfunction in children with idiopathic nephrotic syndrome [science:article] |
Transplant? proc | Cardiovascular?Risk Factors?and?Markers?of?Atherosclerosis?in?Stable?Kidney TransplantRecipients. [pubmed:27496444] |
Biomed Journal | Association of endothelial dysfunction and cytotoxin-associated gene A-positive Helicobacter pylori in patients with cardiac syndrome X. [pubmed:27884380] |
International Journal of Advanced Research in Biological Sciences | Comparative studies on lens structure and function of newborn rats maternally treated with aspartame, monosodium glutamate and galactose [pdfcopy:42552] |
Journal of Drug Metabolisn& Toxicology | Abnormal Retinal Structure and Function of Mother Wistar Rats Supplemented Aspartame, Glutamate and Galactose [587e5b6508aed3826af45e2d.pdf] |
Polish?Journal of Veterinary?Sciences | Antioxidant and anti-inflammatory activities of a commercial noni juice revealed by carrageenan-induced paw edema [pubmed:27760029] |
Artery Research | Endothelin 1 and endothelial dysfunction in children with idiopathic nephrotic syndrome [article:S1872-9312(16)30085-0] |
Journal of Molecular Biomarkers J & Diagnosis | Biochemical and Molecular Markers of Congenital and Senile Cataractous Lenses [DOI: 10.4172/2155-9929.1000318] |
A Study of Effect of Experimental Pre-Eclampsia on Plasma Lipocalin-2 Level in Rats. [AN=123819331] | |
Journal of Functional Foods | Antihypertensive effects of?Ocimum gratissimumextract: Angiotensin-converting enzyme inhibitor in vitro and in vivo investigation [10.1016/j.jff.2017.05.033] |
Radiation?Research | Selective Insulin-like Growth Factor Resistance Associated with Heart Hemorrhages and Poor Prognosis in a Novel Preclinical Model of the Hematopoietic Acute?… [Pubmed:29809108] |
Scandinavian Journal of?Clinical?and Laboratory Investigation | Isolated kidney perfusion: the influence of pulsatile flow [Pubmed:29301417] |
Endokrynologia Polska | Endothelin-1 (ET-1), N-terminal fragment of proatrial natriuretic peptide (NTpro-ANP) and tumor necrosis factor alpha (TNF-α) in children with primary hypertension?… [Pubmed: 30359461] |
Journal of Pharmacological?Sciences | Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndrome [Pubmed: 30342783] |
Molecular Nutrition &?Food?Research | Short‐Term Isocaloric Intake of a Fructose‐but not Glucose‐Rich Diet Affects Bacterial Endotoxin Concentrations and Markers of Metabolic Health in Normal Weight?… [Pubmed: 30570214] |
Journal of Cosmetic Dermatology | Whitening effects of cosmetic formulation in the vascular component of skin pigmentation [Pubmed: 31074159] |
Eurasian Journal of Clinical Sciences | APASL Single Topic Conference DELTA HEPATITIS, 27-28 June 2019, Baku, Azerbaijan [] |
Journal of Functional Foods | Potent anti-inflammatory activity of polysaccharides extracted from Blidingia minima and their effect in a mouse model of inflammatory bowel disease [] |
Scientific Reports | Integrated metabolomics and gut microbiome to the effects and mechanisms of naoxintong capsule on type 2 diabetes in rats [Pubmed: 32616735] |
Evidence-Based Complementary and Alternative Medicine | Tianma Gouteng Decoction Exerts Cardiovascular Protection by Upregulating OPG and TRAIL in Spontaneously Hypertensive Rats [Pubmed: 33133215] |
Microvasc Res | Effect of liraglutide on microcirculation in rat model with absolute insulin deficiency [34119534] |
Biomed Pharmacother | Tianma Gouteng Decoction regulates oxidative stress and inflammation in AngII-induced hypertensive mice via transcription factor EB to exert anti?… [34736077] |
J Ethnopharmacol | Bai-Hu-Tang regulates endothelin-1 and its signalling pathway in vascular endothelial cells [34752899] |
J Ethnopharmacol | Post-stroke treatment of storax improves long-term outcomes of stroke in rats [34343648] |
Optical Technologies for Biology and Medicine | Laser Doppler flowmetry in assessing the effect of liraglutide on microcirculation in rats with absolute insulin deficiency [] |