組氨酸豐富糖蛋白(HRG)單克隆抗體
Monoclonal Antibody to Histidine Rich Glycoprotein (HRG)
HPRG; HRGP; Histidine-Proline Rich Glycoprotein; Thrombophilia Due To Elevated HRG
- 編號MAC534Ra26
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C7
- Ig亞型 IgG2b Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 EPC534Ra61-組氨酸豐富糖蛋白(HRG)真核蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/mL
- 且適物種-
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 756 ¥ 1764 ¥ 2520 ¥ 6300 ¥ 25200
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Sample: Rat Kidney Tissue; Primary Ab: 10μg/ml Mouse Anti-Rat HRG Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Rat Spinal cord Tissue;
Primary Ab: 10μg/ml Mouse Anti-Rat HRG Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Rat Lung Tissue;
Primary Ab: 10μg/ml Mouse Anti-Rat HRG Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Rat Testis Tissue;
Primary Ab: 10μg/ml Mouse Anti-Rat HRG Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Rat Heart Tissue;
Primary Ab: 10μg/ml Mouse Anti-Rat HRG Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Rat Small intestine Tissue;
Primary Ab: 10μg/ml Mouse Anti-Rat HRG Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Rat Testis Tissue
Primary Ab: 10μg/ml Mouse Anti-Rat HRG Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Rat Lung Tissue
Primary Ab: 10μg/ml Mouse Anti-Rat HRG Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Rat Kidney Tissue
Primary Ab: 10μg/ml Mouse Anti-Rat HRG Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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Western Blot; Sample: Lane1: Rat Serum; Lane2: Rat Plasma; Lane3: Rat Pancreas lysate; Lane4: Rat Liver lysate Primary Ab: 5μg/ml Mouse Anti-Rat HRG Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對HRG的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別HRG。
用法
Western blotting: 0.5-5μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APC534Ra61 | 組氨酸豐富糖蛋白(HRG)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPC534Ra01 | 組氨酸豐富糖蛋白(HRG)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPC534Ra61 | 組氨酸豐富糖蛋白(HRG)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC534Ra01 | 組氨酸豐富糖蛋白(HRG)多克隆抗體 | WB; IHC; ICC; IP. |
| MAC534Ra21 | 組氨酸豐富糖蛋白(HRG)單克隆抗體 | WB; IHC |
| MAC534Ra24 | 組氨酸豐富糖蛋白(HRG)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC534Ra25 | 組氨酸豐富糖蛋白(HRG)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC534Ra26 | 組氨酸豐富糖蛋白(HRG)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC534Ra27 | 組氨酸豐富糖蛋白(HRG)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC534Ra29 | 組氨酸豐富糖蛋白(HRG)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC534Ra22 | 組氨酸豐富糖蛋白(HRG)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC534Ra23 | 組氨酸豐富糖蛋白(HRG)單克隆抗體 | WB; IHC |
| FAC534Ra02 | 抗組氨酸豐富糖蛋白(HRG)單克隆抗體 | Flow cytometry. |
| SEC534Ra | 組氨酸豐富糖蛋白(HRG)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC534Ra | 組氨酸豐富糖蛋白(HRG)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| J. Proteome Res. | Identification and Confirmation of Differentially Expressed Fucosylated Glycoproteins in the Serum of Ovarian Cancer Patients Using a Lectin Array and LC–MS/MS [ACS: pr300330z] |
| Medical Oncology | Evaluation of histidine-rich glycoprotein tissue RNA and serum protein as novel markers for breast cancer [Springer: Source] |
| Int J Cancer | The nuclear bile acid receptor FXR controls the liver derived tumor suppressor histidine‐rich glycoprotein [PubMed: 25363753] |
| Nucleic Acid Therapeutics | Pharmacokinetic and Pharmacodynamic Investigations of ION-353382, a Model Antisense Oligonucleotide: Using Alpha-2-Macroglobulin and Murinoglobulin Double-Knockout Mice [Pubmed:27031383] |
| United States Patent Application | Compositions and Methods for Diagnosing Lung Cancer [y2016:0077095.html] |
| Hypertension?Research | Combination of serum histidine-rich glycoprotein and uterine artery Doppler to predict preeclampsia [Pubmed:29416108] |
| journal of histochemistry & cytochemistry | Ultrastructural Localization of Histidine-rich Glycoprotein in Skeletal Muscle Fibers: Colocalization With AMP Deaminase [Pubmed: 31880188] |
| DOKLADY BIOCHEMISTRY AND BIOPHYSICS | Personalized Approach to Determination of Histidine-Rich Glycoprotein and E-Cadherin in Supernatants of Immunocompetent Blood Cells and Breast Biopsy … [Pubmed: 32342301] |
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