抗增殖細胞核抗原(PCNA)單克隆抗體
Monoclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA)
Cyclin
- 編號MAA591Hu21
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C3
- Ig亞型 IgG1 Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標記物無標記物
- 免疫原 RPA591Hu01-抗增殖細胞核抗原(PCNA)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/mL
- 且適物種Cavia (Guinea pig ，豚鼠), Sus scrofa; Porcine (Pig，豬), Bos taurus; Bovine (Cattle，牛), Capra hircus; Caprine (Goat，山羊), Ovis aries; Ovine (Sheep，綿羊), Equus caballus; Equine (Horse，馬)
- 應用WB; IHC; ICC; IP.
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 752 ¥ 1756 ¥ 2508 ¥ 6270 ¥ 25080
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產品包裝(模擬)
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產品包裝(模擬)
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DAB staining on IHC-P; Sample: Human Breast cancer Tissue; Primary Ab: 20μg/ml Mouse Anti-Human PCNA Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Breast cancer Tissue
Primary Ab: 20μg/ml Mouse Anti-Human PCNA Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
FITC staining on IF;
Sample: MCF7 cell
Primary Ab: 20μg/ml Mouse Anti-Human PCNA Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11) -
FITC staining on IF;
Samples: Human MCF7 cell;
Primary Ab: 20μg/ml Mouse Anti-Human PCNA Antibody
Second Ab: 0.75μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu18)
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Figure. Western Blot; Sample: Recombinant PCNA.
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Western Blot Sample: Lane1: Porcine Kidney lysate; Lane2: Bovine Testis lysate; Lane3: Caprine Lung lysate; Lane4: Caprine Testis lysate; Lane5: Cavia Lung lysate; Lane6: Cavia Colon lysate; Lane7: Cavia Thymus lysate; Lane8: Equine Liver lysate
Primary Ab: 1μg/ml Mouse Anti-Human PCNA Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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通過ISO 9001、ISO 13485質量體系認證
特異性
該抗體是針對PCNA的小鼠單克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別PCNA。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
贈品
增值服務
相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA591Hu01 | 抗增殖細胞核抗原(PCNA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CAA591Hu01 | 抗抗增殖細胞核抗原(PCNA)多克隆抗體 | Loading Control of WB. |
| PAA591Hu01 | 抗增殖細胞核抗原(PCNA)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA591Hu21 | 抗增殖細胞核抗原(PCNA)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA591Hu22 | 抗增殖細胞核抗原(PCNA)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA591Hu23 | 抗增殖細胞核抗原(PCNA)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA591Hu25 | 抗增殖細胞核抗原(PCNA)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA591Hu24 | 抗增殖細胞核抗原(PCNA)單克隆抗體 | WB; IHC; ICC; IP. |
| CAA591Hu22 | 抗抗增殖細胞核抗原(PCNA)單克隆抗體 | Loading Control of WB. |
| KSA591Hu01 | 抗增殖細胞核抗原(PCNA)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Tissue and Cell | Human umbilical cord blood-mesenchymal stem cells transplantation renovates the ovarian surface epithelium in a rat model of premature ovarian failure: Possible direct and indirect effects [Pubmed:27233913] |
| Food and Chemical Toxicology | Antioxidant, anti-inflammatory and synergistic anti-hyperglycemic effects of Malaysian propolis and metformin in streptozotocin–induced diabetic rats [Pubmed:30026088] |
| Life?Sciences | Malaysian propolis, metformin and their combination, exert hepatoprotective effect in streptozotocin-induced diabetic rats [Pubmed: 30205096] |
| Archives of Physiology and Biochemistry | Diabetes-induced testicular oxidative stress, inflammation, and caspase-dependent apoptosis: the protective role of metformin [Pubmed: 30513216] |
| European journal of medicinal chemistry | Synthesis of novel S-linked dihydroartemisinin derivatives and evaluation of their anticancer activity [Pubmed: 31216504] |
| Antioxidants (Basel). | The Effect of Dietary Quercetin on the Glutathione Redox System and Small Intestinal Functionality of Weaned Piglets [Pubmed: 31426309] |
| Antioxidants | Oxidative Stress, NF-κB-Mediated Inflammation and Apoptosis in the Testes of Streptozotocin–Induced Diabetic Rats: Combined Protective Effects of Malaysian?… [Pubmed: 31600920] |
| TOXICOLOGY | Tert-butylhydroquinone preserve testicular steroidogenesis and spermatogenesis in cisplatin-intoxicated rats by targeting oxidative stress, inflammation and apoptosis [Pubmed: 32565124] |
| BIOMEDICINE & PHARMACOTHERAPY | Protective effects of bee bread on testicular oxidative stress, NF-κB-mediated inflammation, apoptosis and lactate transport decline in obese male rats [Pubmed: 33152939] |
| Sci Rep | Tert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes, and improves spermatogenesis in rats [33750916] |
| J Cell Sci | Local DNA synthesis is critical for DNA repair during oocyte maturation [34415018] |
| Stem Cell Rev Rep | Comparative Evaluation of Anti-Fibrotic Effect of Tissue Specific Mesenchymal Stem Cells Derived Extracellular Vesicles for the Amelioration of CCl4 Induced Chronic?… [34859376] |
| Naunyn-Schmiedeberg's Archives | Inflammatory Modulation of miR-155 Inhibits Doxorubicin?Induced Testicular Dysfunction Via Sirt1/Foxo1 Pathway: Insight to the Role of Acacetin and Bacillus?… [Pubmed:35715546] |
| Antioxidants | Therapeutic Effects of Bee Bread on Obesity-Induced Testicular-Derived Oxidative Stress, Inflammation, and Apoptosis in High-Fat Diet Obese Rat Model?… [Pubmed:35204140] |
| Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β?… [] | |
| Impact de l'incorporation d'acides gras à chaine moyenne, de suppléments de levures et d'avoine nue sur la santé intestinale ainsi que sur la consommation?… [] |
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