抗增殖細(xì)胞核抗原(PCNA)單克隆抗體
Monoclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA)
Cyclin
- 編號MAA591Mi23
- 物種Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Sus scrofa; Porcine (Pig，豬) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C8
- Ig亞型 IgG1 Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPA591Mi01-抗增殖細(xì)胞核抗原(PCNA)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度1mg/mL
- 且適物種Cavia (Guinea pig ，豚鼠), Capra hircus; Caprine (Goat，山羊), Ovis aries; Ovine (Sheep，綿羊), Chicken (Gallus，雞)
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 770 ¥ 1798 ¥ 2568 ¥ 6420 ¥ 25680
- 欲了解實(shí)際交易價格和更多情況，請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
DAB staining on IHC-P; Sample: Human Breast cancer Tissue; Primary Ab: 20μg/ml Mouse Anti-Multi-species PCNA Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
-
Western Blot; Sample: Lane1: Mouse Testis lysate; Lane2: Jurkat cell lysate; Lane3: Rat Testis lysate Primary Ab: 3μg/ml Mouse Anti-Multi-species PCNA Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
-
Western Blot; Sample: Lane1: A549 cell lysate; Lane2: 293T cell lysate; Lane3: A875 cell lysate; Lane4: HepG2 cell lysate; Lane5: Hela cell lysate; Lane6: YAC1 cell lysate; Lane7: MCF7 cell lysate
Primary Ab: 1μg/ml Mouse Anti-Multi-species PCNA Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Lane1: Sp2/0 cell lysate; Lane2: SGC7901 cell lysate; Lane3: ECV304 cell lysate; Lane4: PC3 cell lysate; Lane5: A375 cell lysate; Lane6: A431 cell lysate; Lane7: Jurkat cell lysate
Primary Ab: 1μg/ml Mouse Anti-Multi-species PCNA Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Lane1: Mouse Small intestine lysate; Lane2: Mouse Lymph node lysate; Lane3: Mouse Ovary lysate; Lane4: Mouse Thymus lysate
Primary Ab: 1μg/ml Mouse Anti-Multi-species PCNA Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Lane1: Rat Spleen lysate; Lane2: Porcine Heart lysate; Lane3: Porcine Liver lysate; Lane4: Porcine Spleen lysate; Lane5: Cavia Spleen lysate; Lane6: Caprine Heart lysate; Lane7: Caprine Liver lysate; Lane8: Gallus Cerebrum lysate
Primary Ab: 1μg/ml Mouse Anti-Multi-species PCNA Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
-
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對PCNA的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識別PCNA。
用法
Western blottin:g: 0.01-3μg/mL;
Immunohistochemistry: 5-30μg/mL;
Immunocytochemistry: 5-30μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽性對照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長臂生物素標(biāo)記試劑盒
實(shí)時熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Sus scrofa; Porcine (Pig，豬) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA591Mi01 | 抗增殖細(xì)胞核抗原(PCNA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA591Mi01 | 抗增殖細(xì)胞核抗原(PCNA)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA591Mi22 | 抗增殖細(xì)胞核抗原(PCNA)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA591Mi23 | 抗增殖細(xì)胞核抗原(PCNA)單克隆抗體 | WB; IHC; ICC; IP. |
| CAA591Mi22 | 抗抗增殖細(xì)胞核抗原(PCNA)單克隆抗體 | Loading Control of WB |
| MAA591Mi24 | 抗增殖細(xì)胞核抗原(PCNA)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA591Mi21 | 抗增殖細(xì)胞核抗原(PCNA)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA591Mi | 抗增殖細(xì)胞核抗原(PCNA)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA591Mi | 抗增殖細(xì)胞核抗原(PCNA)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Tissue and Cell | Human umbilical cord blood-mesenchymal stem cells transplantation renovates the ovarian surface epithelium in a rat model of premature ovarian failure: Possible direct and indirect effects [Pubmed:27233913] |
| Food and Chemical Toxicology | Antioxidant, anti-inflammatory and synergistic anti-hyperglycemic effects of Malaysian propolis and metformin in streptozotocin–induced diabetic rats [Pubmed:30026088] |
| Life?Sciences | Malaysian propolis, metformin and their combination, exert hepatoprotective effect in streptozotocin-induced diabetic rats [Pubmed: 30205096] |
| Archives of Physiology and Biochemistry | Diabetes-induced testicular oxidative stress, inflammation, and caspase-dependent apoptosis: the protective role of metformin [Pubmed: 30513216] |
| European journal of medicinal chemistry | Synthesis of novel S-linked dihydroartemisinin derivatives and evaluation of their anticancer activity [Pubmed: 31216504] |
| Antioxidants (Basel). | The Effect of Dietary Quercetin on the Glutathione Redox System and Small Intestinal Functionality of Weaned Piglets [Pubmed: 31426309] |
| Antioxidants | Oxidative Stress, NF-κB-Mediated Inflammation and Apoptosis in the Testes of Streptozotocin–Induced Diabetic Rats: Combined Protective Effects of Malaysian?… [Pubmed: 31600920] |
| TOXICOLOGY | Tert-butylhydroquinone preserve testicular steroidogenesis and spermatogenesis in cisplatin-intoxicated rats by targeting oxidative stress, inflammation and apoptosis [Pubmed: 32565124] |
| BIOMEDICINE & PHARMACOTHERAPY | Protective effects of bee bread on testicular oxidative stress, NF-κB-mediated inflammation, apoptosis and lactate transport decline in obese male rats [Pubmed: 33152939] |
| Sci Rep | Tert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes, and improves spermatogenesis in rats [33750916] |
| J Cell Sci | Local DNA synthesis is critical for DNA repair during oocyte maturation [34415018] |
| Stem Cell Rev Rep | Comparative Evaluation of Anti-Fibrotic Effect of Tissue Specific Mesenchymal Stem Cells Derived Extracellular Vesicles for the Amelioration of CCl4 Induced Chronic?… [34859376] |
| Naunyn-Schmiedeberg's Archives | Inflammatory Modulation of miR-155 Inhibits Doxorubicin?Induced Testicular Dysfunction Via Sirt1/Foxo1 Pathway: Insight to the Role of Acacetin and Bacillus?… [Pubmed:35715546] |
| Antioxidants | Therapeutic Effects of Bee Bread on Obesity-Induced Testicular-Derived Oxidative Stress, Inflammation, and Apoptosis in High-Fat Diet Obese Rat Model?… [Pubmed:35204140] |
| Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β?… [] | |
| Impact de l'incorporation d'acides gras à chaine moyenne, de suppléments de levures et d'avoine nue sur la santé intestinale ainsi que sur la consommation?… [] |
留言咨詢
