分泌型卷曲相關(guān)蛋白1(SFRP1)單克隆抗體
Monoclonal Antibody to Secreted Frizzled Related Protein 1 (SFRP1)
FRP1; FRP; FRP-1; FrzA; SARP2; Secreted apoptosis-related protein 2
- 編號(hào)MAF880Hu24
- 物種Homo sapiens (Human,人) 相同的名稱(chēng),不同的物種。
- 來(lái)源單抗制備
- 宿主小鼠
- 效價(jià)(克隆號(hào))C9
- Ig亞型 IgG1 Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無(wú)標(biāo)記物
- 免疫原 RPF880Hu01-分泌型卷曲相關(guān)蛋白1(SFRP1)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液(pH7.4,containing 0.05% Proclin-300,50% glycerol)
- 性狀液體
- 濃度1mg/mL
- 且適物種Sus scrofa; Porcine (Pig,豬)
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù),請(qǐng)參見(jiàn)流式抗體。 - 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 670 ¥ 1562 ¥ 2232 ¥ 5580 ¥ 22320
- 欲了解實(shí)際交易價(jià)格和更多情況,請(qǐng)與當(dāng)?shù)亟?jīng)銷(xiāo)商聯(lián)系!
特異性
該抗體是針對(duì)SFRP1的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別SFRP1。
用法
Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽(yáng)性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長(zhǎng)臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
編號(hào) | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPF880Hu01 | 分泌型卷曲相關(guān)蛋白1(SFRP1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAF880Hu01 | 分泌型卷曲相關(guān)蛋白1(SFRP1)多克隆抗體 | WB; IHC; ICC; IP. |
MAF880Hu21 | 分泌型卷曲相關(guān)蛋白1(SFRP1)單克隆抗體 | WB,IHC |
MAF880Hu22 | 分泌型卷曲相關(guān)蛋白1(SFRP1)單克隆抗體 | WB; IHC; ICC; IP. |
MAF880Hu23 | 分泌型卷曲相關(guān)蛋白1(SFRP1)單克隆抗體 | WB; IHC; ICC; IP. |
MAF880Hu24 | 分泌型卷曲相關(guān)蛋白1(SFRP1)單克隆抗體 | WB; IHC; ICC; IP. |
MAF880Hu25 | 分泌型卷曲相關(guān)蛋白1(SFRP1)單克隆抗體 | WB; IHC; ICC; IP. |
SEF880Hu | 分泌型卷曲相關(guān)蛋白1(SFRP1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMF880Hu | 分泌型卷曲相關(guān)蛋白1(SFRP1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
BMC Musculoskeletal Disorders | Biomarkers and cytokines of bone turnover: extensive evaluation in a cohort of patients with ankylosing spondylitis [PubMed: 23025387] |
Exp Cell Res | Wnt Inhibition is Dysregulated in Gliomas and its Re-Establishment Inhibits Proliferation and Tumor Sphere Formation [PubMed: 26712519] |
BMC Musculoskeletal Disorders | Correlation?of?blood?bone?turnover?biomarkers?and?Wnt?signaling?antagonists?with AS,?DISH,OPLL, and?OYL. [pubmed:28153008] |
PloS one | Effects of exercise training on circulating levels of Dickkpof-1 and secreted frizzled-related protein-1 in breast cancer survivors: A pilot single-blind randomized controlled trial. [pubmed:28178355] |
PLoS One | Effects of exercise training on circulating levels of Dickkpof-1 and secreted frizzled-related protein-1 in breast cancer survivors: A pilot single-blind randomized controlled trial [10.1371] |
Journal of Experimental & Clinical Cancer Research | PHF21B overexpression promotes cancer stem cell-like traits in prostate cancer cells by activating the Wnt/β-catenin signaling pathway [pubmed:28645312] |
JBMR Plus | Low serum levels of DKK2 predict incident low‐impact fracture in older women [Doi: 10.1002/jbm4.10179] |
Nature Communications | Immunological history governs human stem cell memory CD4 heterogeneity via the Wnt signaling pathway [Pubmed: 32041953] |
acta pharmacologica sinica | sFRP1 protects H9c2 cardiac myoblasts from doxorubicin-induced apoptosis by inhibiting the Wnt/PCP-JNK pathway [Pubmed: 32238888] |