分泌型卷曲相關(guān)蛋白1(SFRP1)單克隆抗體
Monoclonal Antibody to Secreted Frizzled Related Protein 1 (SFRP1)
FRP1; FRP; FRP-1; FrzA; SARP2; Secreted apoptosis-related protein 2
- 編號MAF880Ra22
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C5
- Ig亞型 IgG3 Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPF880Ra01-分泌型卷曲相關(guān)蛋白1(SFRP1)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/ml
- 且適物種-
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 738 ¥ 1722 ¥ 2460 ¥ 6150 ¥ 24600
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Sample: Rat Uterus Tissue; Primary Ab: 30μg/ml Mouse Anti-Rat SFRP1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Rat Cardiac Muscle Tissue;
Primary Ab: 30μg/ml Mouse Anti-Rat SFRP1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Rat Testis Tissue;
Primary Ab: 30μg/ml Mouse Anti-Rat SFRP1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Rat Liver Tissue;
Primary Ab: 30μg/ml Mouse Anti-Rat SFRP1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對SFRP1的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別SFRP1。
用法
Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APF880Ra01 | 分泌型卷曲相關(guān)蛋白1(SFRP1)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPF880Ra01 | 分泌型卷曲相關(guān)蛋白1(SFRP1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAF880Ra01 | 分泌型卷曲相關(guān)蛋白1(SFRP1)多克隆抗體 | WB; IHC; ICC; IP. |
| LAF880Ra71 | 分泌型卷曲相關(guān)蛋白1(SFRP1)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAF880Ra24 | 分泌型卷曲相關(guān)蛋白1(SFRP1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAF880Ra22 | 分泌型卷曲相關(guān)蛋白1(SFRP1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAF880Ra25 | 分泌型卷曲相關(guān)蛋白1(SFRP1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAF880Ra23 | 分泌型卷曲相關(guān)蛋白1(SFRP1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAF880Ra21 | 分泌型卷曲相關(guān)蛋白1(SFRP1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAF880Ra27 | 分泌型卷曲相關(guān)蛋白1(SFRP1)單克隆抗體 | WB; IHC; ICC; IP. |
| SEF880Ra | 分泌型卷曲相關(guān)蛋白1(SFRP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMF880Ra | 分泌型卷曲相關(guān)蛋白1(SFRP1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| BMC Musculoskeletal Disorders | Biomarkers and cytokines of bone turnover: extensive evaluation in a cohort of patients with ankylosing spondylitis [PubMed: 23025387] |
| Exp Cell Res | Wnt Inhibition is Dysregulated in Gliomas and its Re-Establishment Inhibits Proliferation and Tumor Sphere Formation [PubMed: 26712519] |
| BMC Musculoskeletal Disorders | Correlation?of?blood?bone?turnover?biomarkers?and?Wnt?signaling?antagonists?with AS,?DISH,OPLL, and?OYL. [pubmed:28153008] |
| PloS one | Effects of exercise training on circulating levels of Dickkpof-1 and secreted frizzled-related protein-1 in breast cancer survivors: A pilot single-blind randomized controlled trial. [pubmed:28178355] |
| PLoS One | Effects of exercise training on circulating levels of Dickkpof-1 and secreted frizzled-related protein-1 in breast cancer survivors: A pilot single-blind randomized controlled trial [10.1371] |
| Journal of Experimental & Clinical Cancer Research | PHF21B overexpression promotes cancer stem cell-like traits in prostate cancer cells by activating the Wnt/β-catenin signaling pathway [pubmed:28645312] |
| JBMR Plus | Low serum levels of DKK2 predict incident low‐impact fracture in older women [Doi: 10.1002/jbm4.10179] |
| Nature Communications | Immunological history governs human stem cell memory CD4 heterogeneity via the Wnt signaling pathway [Pubmed: 32041953] |
| acta pharmacologica sinica | sFRP1 protects H9c2 cardiac myoblasts from doxorubicin-induced apoptosis by inhibiting the Wnt/PCP-JNK pathway [Pubmed: 32238888] |
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