胱天蛋白酶8(CASP8)多克隆抗體
Polyclonal Antibody to Caspase 8 (CASP8)
CAP4; FLICE; MACH; MCH5; Cysteinyl Aspartate Specific Proteinases 8; Apoptotic cysteine protease; FADD-homologous ICE/ced-3-like protease; MORT1-associated ced-3 homolog
- 編號PAA853Hu03
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPA853Hu03-胱天蛋白酶8(CASP8)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB,IHC,ICC/IF
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 504 ¥ 1176 ¥ 1680 ¥ 4200 ¥ 16800
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Liver cancer Tissue; Primary Ab: 30ug/ml Rabbit Anti-Human CASP8 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Lymphoma Tissue;
Primary Ab: 30ug/ml Rabbit Anti-Human CASP8 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 30ug/ml Rabbit Anti-Human CASP8 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Lymph node Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human CASP8 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Spleen Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human CASP8 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Liver Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human CASP8 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Colon Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human CASP8 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Figure: FITC staining on IHC-P;
Sample: Hela cells. -
FITC staining on IF;
Samples: Human HepG2 cell;
Primary Ab: 20μg/ml Rabbit Anti-Human CASP8 Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18) -
FITC staining on IF;
Sample: HepG2 cell
Primary Ab: 20μg/ml Rabbit Anti-Human CASP8 Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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Figure. Western Blot; Sample: Recombinant CASP8, Human.
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Western Blot; Sample: Lane1: Human Liver lysate; Lane2: HepG2 cell lysate; Lane3: Hela cell lysate; Lane4: Jurkat cell lysate; Lane5: Raji cell lysate; Lane6: Porcine Liver lysate
Primary Ab: 0.2μg/ml Rabbit Anti-Human CASP8 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification: <br/>Lane 1: Wild-type Hela cell lysate; <br/>Lane 2: CASP8 knockout Hela cell lysate; <br/>Predicted MW: 26-32,46,54-62kDa <br/>Observed MW: 60kDa<br/>Primary Ab: 3μg/ml Rabbit Anti-Human CASP8 Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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Western?blot?shows?lysates?of?Jurkat?cell?line?treated?with?1?μM?staurosporine?(STS)?for?the?indicated?times.?
Primary?Ab:?1ug/ml?Rabbit?Anti-Human?CASP8?Antibody
Second?Ab:?0.2μg/mL?HRP-Linked?Caprine?Anti-Rabbit?IgG?Polyclonal?Antibody
(Catalog:?SAA544Rb19)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對CASP8的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別CASP8。
用法
Western blotting: 0.01-3μg/mL;
Immunohistochemistry: 5-30μg/mL;
Immunocytochemistry: 5-30μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA853Hu02 | 胱天蛋白酶8(CASP8)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA853Hu03 | 胱天蛋白酶8(CASP8)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA853Hu01 | 胱天蛋白酶8(CASP8)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA853Hu02 | 胱天蛋白酶8(CASP8)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA853Hu04 | 胱天蛋白酶8(CASP8)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA853Hu01 | 胱天蛋白酶8(CASP8)多克隆抗體 | WB,IHC,ICC/IF |
| PAA853Hu03 | 胱天蛋白酶8(CASP8)多克隆抗體 | WB,IHC,ICC/IF |
| FAA853Hu01 | 抗胱天蛋白酶8(CASP8)多克隆抗體 | Flow cytometry. |
| LAA853Hu81 | 胱天蛋白酶8(CASP8)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAA853Hu71 | 胱天蛋白酶8(CASP8)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAA853Hu21 | 胱天蛋白酶8(CASP8)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA853Hu23 | 胱天蛋白酶8(CASP8)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA853Hu25 | 胱天蛋白酶8(CASP8)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA853Hu24 | 胱天蛋白酶8(CASP8)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA853Hu29 | 胱天蛋白酶8(CASP8)單克隆抗體 | WB |
| MAA853Hu27 | 胱天蛋白酶8(CASP8)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA853Hu22 | 胱天蛋白酶8(CASP8)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA853Hu | 胱天蛋白酶8(CASP8)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA853Hu | 胱天蛋白酶8(CASP8)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA853Hu01 | 胱天蛋白酶8(CASP8)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Molecular Neurobiology | Ursodeoxycholic Acid Ameliorates Apoptotic Cascade in the Rotenone Model of Parkinson’s Disease: Modulation of Mitochondrial Perturbations [Pubmed:25502462] |
| Journal of Ethnopharmacology | Viscum articulatum Burm. f. aqueous extract exerts antiproliferative effect and induces cell cycle arrest and apoptosis in leukemia cells [Pubmed:29555410] |
| Clinical?And Experimental Pharmacology And Physiology? | Diallyl sulfide alleviates cisplatin‐induced nephrotoxicity in rats via suppressing NF‐κB downstream inflammatory proteins and p53/Puma signalling pathway [Pubmed:29266336] |
| Environmental Toxicology | Antihepatocarcinogenic activity of whey protein concentrate and lactoferrin in diethylnitrosamine‐treated male albino mice [Pubmed: 31087429] |
| Environ Toxicol Pharmacol. | Targetingp53/TRAIL/caspase-8 signaling by adiponectin reverses thioacetamide-induced hepatocellular carcinoma in rats [Pubmed: 31421311] |
| Journal of Biochemical and Molecular Toxicology | Tranilast attenuates methotrexate‐induced renal and hepatic toxicities: Role of apoptosis‐induced tissue proliferation [Pubmed: 32045101] |
| life sciences | Nifuroxazide attenuates experimentally-induced hepatic encephalopathy and the associated hyperammonemia and cJNk/caspase-8/TRAIL activation in rats [Pubmed: 32259601] |
| Odontology | Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells [Pubmed: 33206337] |
| Int Immunopharmacol | Cardio-protective impact of gabapentin against doxorubicin-induced myocardial toxicity in rats; emphasis on modulation of inflammatory-apoptotic signaling [Pubmed: 33199237] |
| ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | Chemo-preventive effect of crocin against experimentally-induced hepatocarcinogenesis via regulation of apoptotic and Nrf2 signaling pathways [Pubmed: 32942000] |
| Environ Sci Pollut Res Int | Potentiation of the apoptotic signaling pathway in both the striatum and hippocampus and neurobehavioral impairment in rats exposed chronically to a low? dose of?… [Pubmed: 32915453] |
| J Biochem Mol Toxicol | Tranilast abrogates cisplatin?\induced testicular and epididymal injuries: An insight into its modulatory impact on apoptosis/proliferation [34047436] |
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