細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(CD147)多克隆抗體
Polyclonal Antibody to Cluster Of Differentiation 147 (CD147)
EMMPRIN; BSG; 5F7; M6; OK; TCSF; Basigin(Ok Blood Group); Collagenase stimulatory factor; OK blood group antigen; Tumor cell-derived collagenase stimulatory factor
- 編號(hào)PAB540Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 來(lái)源多抗制備
- 宿主兔
- 效價(jià)-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無(wú)標(biāo)記物
- 免疫原 RPB540Mu01-細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(CD147)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度500μg/mL
- 且適物種Homo sapiens (Human，人), Rattus norvegicus (Rat，大鼠)
- 應(yīng)用WB; IHC; ICC; IP; FCM
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見(jiàn)流式抗體。 - 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 616 ¥ 1436 ¥ 2052 ¥ 5130 ¥ 20520
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Mouse Liver Tissue; Primary Ab: 10μg/ml Rabbit Anti-Mouse EMMPRIN Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Mouse Testis Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Mouse EMMPRIN Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Kidney Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Mouse EMMPRIN Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Heart Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Mouse EMMPRIN Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Uterus Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Mouse EMMPRIN Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Intestine Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Mouse EMMPRIN Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant EMMPRIN, Mouse.
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Western Blot; Sample: Lane1: Mouse Liver lysate; Lane2: Mouse Cerebrum lysate; Lane3: Hela cell lysate; Lane4: HepG2 cell lysate
Primary Ab: 0.2μg/ml Rabbit Anti-Mouse CD147 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Rat Heart lysate
Primary Ab: 0.2μg/ml Rabbit Anti-Mouse EMMPRIN Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Mouse Thymocytes cell were stained with 20μg/ml rabbit Anti-mouse CD147 Polyclonal Antibody (Catalog PAB540Mu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)CD147的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別CD147。
用法
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽(yáng)性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長(zhǎng)臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APB540Mu61 | 細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(CD147)活性蛋白 | Cell?culture;?Activity?Assays. |
| APB540Mu02 | 細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(CD147)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB540Mu01 | 細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(CD147)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB540Mu02 | 細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(CD147)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB540Mu01 | 細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(CD147)活性蛋白 | Cell?culture;?Activity?Assays. |
| EPB540Mu61 | 細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(CD147)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB540Mu01 | 細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(CD147)多克隆抗體 | WB; IHC; ICC; IP; FCM |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| DISEASE MARKERS | The Combination of CD147 and MMP-9 Serum Levels Is Identified as Novel Chemotherapy Response Markers of Advanced Non-Small-Cell Lung Cancer [Pubmed: 32377273] |
