胰島素(INS)多克隆抗體
Polyclonal Antibody to Insulin (INS)
- 編號PAA448Rb01
- 物種Oryctolagus cuniculus (Rabbit,兔) 相同的名稱,不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPA448Rb01-胰島素(INS)重組蛋白
- 緩沖液成份磷酸鹽緩沖液(pH7.4,含有0.02% NaN3和50%甘油)
- 性狀液體
- 濃度0.41mg/ml
- 且適物種-
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù),請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 635 ¥ 1483 ¥ 2118 ¥ 5295 ¥ 21180
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特異性
該抗體是針對INS的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別INS。
用法
Western blotting: 0.5-2μg/mL
Immunohistochemistry: 5-20μg/mL
Immunocytochemistry: 5-20μg/mL
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲,損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Oryctolagus cuniculus (Rabbit,兔) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPA448Rb01 | 胰島素(INS)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA448Rb51 | 胰島素(INS)多克隆抗體 | WB; IHC; ICC; IP. |
PAA448Rb01 | 胰島素(INS)多克隆抗體 | WB; IHC; ICC; IP. |
LAA448Rb51 | 胰島素(INS)多克隆抗體(別藻藍(lán)蛋白標(biāo)記) | WB; IHC; ICC; IF. |
CEA448Rb | 胰島素(INS)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA448Rb | 胰島素(INS)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
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Cellular Physiology and Biochemistry | Irisin Regulates Heme Oxygenase-1/ Adiponectin Axis in Perivascular Adipose Tissue and Improves Endothelial Dysfunction in Diet-Induced Obese Mice [pubmed:28595178] |
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Frontiers in Physiology | Ileal Transposition Surgery Decreases Fat Mass and Improves Glucose Metabolism in Diabetic GK Rats: Possible Involvement of FGF21 [Pubmed:29593555] |
Chemico-Biological Interactions | Crocin mediated amelioration of oxidative burden and inflammatory cascade suppresses diabetic nephropathy progression in diabetic rats [Pubmed:29409856] |
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Obesity?Surgery | The Effect of Gastric Bypass with a Distal Gastric Pouch on Glucose Tolerance and Diabetes Remission in Type 2 Diabetes Sprague-Dawley Rat Model [Pubmed: 30778846] |
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Stress | Regulating glycolysis, the TLR4 signal pathway and expression of RBM3 in mouse liver in response to acute cold exposure [Pubmed: 30821572] |
Life?Sciences | Interrelation of liver vascularity to non-alcoholic fatty liver through a comparative study of the vasodilator effect of carvedilol or nicorandil in rats [Pubmed: 30826497] |
cell?biology international | Regulation of insulin resistance by targeting insulin‐like growth factor 1 receptor (IGF‐1R) with microRNA‐122‐5p in hepatic cells [Pubmed: 30958584] |
international?journal?of?molecular?sciences | Effects of Cold-inducible RNA-binding Protein (CIRP) on Liver Glycolysis during Acute Cold Exposure in C57BL/6 Mice [Pubmed: 30909542] |
Journal of Clinical and Translational Endocrinology | Dobesity: relationship between vitamin d deficiency, obesity and sclerostin as a novel biomarker of bone metabolism [] |
Acta Pharmacologica Sinica | Huperzine A ameliorates obesity-related cognitive performance impairments involving neuronal insulin signaling pathway in mice [Pubmed: 31213670] |
Biochemistry | Protective Effect of Peroxiredoxin 6 Against Toxic Effects of Glucose and Cytokines in Pancreatic RIN-m5F β-Cells [] |
Physiological Research | Tempol alleviates chronic intermittent hypoxia-induced pancreatic injury through repressing inflammation and apoptosis. [Pubmed: 31301730] |
molecular nutrition & food research | Glycerol Monolaurate‐Mediated Attenuation of Metabolic Syndrome is Associated with the Modulation of Gut Microbiota in High Fat diet‐fed Mice [Pubmed: 31318165] |
Arch Physiol Biochem | Associations of apelin, leptin, irisin, ghrelin, insulin, glucose levels, and lipid parameters with physical activity during eight weeks of regular exercise training [Pubmed: 31290696] |
Efeito do consumo cr?nico de óleo de coco sobre parametros bioquímicos e do status redox em ratas saudáveis. [] | |
Diabetes-Metabolism Research and Reviews | Liraglutide Combined with Human Umbilical Cord Mesenchymal Stem Cell Transplantation inhibits beta‐cells apoptosis via mediating the ASK1/JNK/BAX pathway in [Pubmed: 31411368] |
Phytomedicine | Baicalin ameliorates hepatic insulin resistance and gluconeogenic activity through inhibition of p38 MAPK/PGC-1α pathway [] |
Naunyn-Schmiedebergs Archives of Pharmacology | Coptisine ameliorates renal injury in diabetic rats through the activation of Nrf2 signaling pathway [Pubmed: 31420722] |
Nutrients | High-dose Glycerol Monolaurate Up-Regulated Beneficial Indigenous Microbiota without Inducing Metabolic Dysfunction and Systemic Inflammation: New Insights?… [] |
Cells | MicroRNA-99a is a Potential Target for Regulating Hypothalamic Synaptic Plasticity in the Peri/Postmenopausal Depression Model [Pubmed: 31540304] |
Molecular and cellular endocrinology | Metformin alleviates oxidative stress and enhances autophagy in diabetic kidney disease via AMPK/SIRT1-FoxO1 pathway [Pubmed: 31647955] |
Obesity Surgery | Duodenal-Jejunal Bypass Ameliorates Type 2 Diabetes Mellitus by Activating Insulin Signaling and Improving Glucose Utilization in the Brain [Pubmed: 31605365] |
Surgical Endoscopy and Other Interventional Techniques | Distal gastric mucosa ablation induces significant weight loss and improved glycemic control in type 2 diabetes Sprague–Dawley rat model [Pubmed: 31630290] |
Biomedicine & Pharmacotherapy | Propolis modulates the gut microbiota and improves the intestinal mucosal barrier function in diabetic rats [Pubmed: 31545258] |
International Journal of Endocrinology | miR-29a Negatively Affects Glucose-Stimulated Insulin Secretion and MIN6 Cell Proliferation via Cdc42/β-Catenin Signaling [Pubmed: 31662747] |
Food & Function | Depolymerized RG-I enriched pectin from citrus segment membrane modulates gut microbiota, increases SCFAs production, promotes the growth of Bifidobacterium?… [Pubmed: 31778135] |
Life Sciences | P-Coumaric acid alleviates experimental diabetic nephropathy through modulation of toll like receptor-4 in rats [Pubmed: 31629762] |
Journal of Ovarian Research | Baicalin ameliorates polycystic ovary syndrome through AMP-activated protein kinase [Pubmed: 31722718] |
journal of ethnopharmacology | Dietary supplementation of Morus nigra L. leaves decrease fat mass partially through elevating leptin-stimulated lipolysis in pig model [Pubmed: 31756448] |
Brain Research | Isoflurane aggravates peripheral and central insulin resistance in high-fat diet/streptozocin-induced type 2 diabetic mice [Pubmed: 31672472] |
Journal of Ethnopharmacology | San-Huang-Tang protects obesity/diabetes induced NAFLD by upregulating PGC-1α/PEPCK signaling in obese and galr1 knockout mice models [Pubmed: 31843573] |
Journal of Hazardous Materials | Triphenyl phosphate causes a sexually dimorphic metabolism dysfunction associated with disordered adiponectin receptors in pubertal mice [Pubmed: 31796355] |
Journal of Steroid Biochemistry and Molecular Biology | SerpinB1 promotes the proliferation of porcine pancreatic stem cells through the STAT3 signaling pathway [Pubmed: 31785377] |
Journal of dermatological treatment | Liraglutide improved inflammation via mediating IL-23/Th-17 pathway in obese diabetic mice with psoriasiform skin [Pubmed: 31868553] |
Nutrients | Rotundic Acid Protects against Metabolic Disturbance and Improves Gut Microbiota in Type 2 Diabetes Rats [Pubmed: 31887996] |
JOURNAL OF DAIRY SCIENCE | Decreased amylolytic microbes of the hindgut and increased blood glucose implied improved starch utilization in the small intestine by using rumen-protected leucine … [Pubmed: 32113753] |
Biomed Pharmacother | Composite probiotics alleviate type 2 diabetes by regulating intestinal microbiota and inducing GLP-1 secretion in db/db mice [Pubmed: 32035395] |
Frontiers in Endocrinology | Hyperbaric oxygen ameliorates insulin sensitivity by increasing GLUT4 expression in skeletal muscle and stimulating UCP1 in brown adipose tissue in T2DM … [Pubmed: 32082261] |
Cell Physiol Biochem | Integrin Linked Kinase (ILK) Downregulation as an Early Event During the Development of Metabolic Alterations in a Short-Term High Fat Diet Mice Model. [Pubmed: 31972071] |
JOURNAL OF FOOD SCIENCE | Beneficial effects of Aronia melanocarpa berry extract on hepatic insulin resistance in type 2 diabetes mellitus rats [Pubmed: 32249934] |
Journal of Diabetes Research | Roux-en-Y Gastric Bypass in Obese Diabetic Rats Promotes Autophagy to Improve Lipid Metabolism through mTOR/p70S6K Signaling Pathway [Pubmed: 32309446] |
Brain Research | Electroacupuncture improves cognitive deficits and insulin resistance in an OLETF rat model of Al/D-gal induced aging model via the PI3K/Akt signaling pathway [Pubmed: 32304687] |
endocrine journal | Mice with nucleus tractus solitarius injury induced by chronic restraint stress present impaired ability to raise blood glucose and glucagon levels when blood glucose?… [Pubmed: 32249244] |
Frontiers in Endocrinology | RFRP-3, the Mammalian Ortholog of GnIH, Is a Novel Modulator Involved in Food Intake and Glucose Homeostasis [Pubmed: 32328034] |
Antioxidants | Effects of Allicin on Pathophysiological Mechanisms during the Progression of Nephropathy Associated to Diabetes [Pubmed: 33203103] |
Nature Communications | AdipoR1/AdipoR2 dual agonist recovers nonalcoholic steatohepatitis and related fibrosis via endoplasmic reticulum-mitochondria axis [Pubmed: 33199780] |
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES | Evaluation of the hypoglycemic effect of exendin-4's new oral self-nanoemulsifying system in rats [Pubmed: 33197556] |
BIOLOGICAL RESEARCH | Novel mechanisms underlying anti-polycystic ovary like syndrome effects of electroacupuncture in rats: suppressing SREBP1 to mitigate insulin resistance?… [Pubmed: 33109277] |
FASEB JOURNAL | Irradiation of carotid baroreceptor with low‐intensity pulsed ultrasound exerts different metabolic protection in perirenal, epididymal white adipose tissue and?… [Pubmed: 32954574] |
Biol Res | Palmatine ameliorates high fat diet induced impaired glucose tolerance [Pubmed: 32928312] |
ENDOCRINE | Thioredoxin-interacting protein: a critical link between autophagy disorders and pancreatic β-cell dysfunction [Pubmed: 32892310] |
DIABETOLOGIA | Adiponectin treatment improves insulin resistance in mice by regulating the expression of the mitochondrial-derived peptide MOTS-c and its response to exercise via?… [Pubmed: 32880686] |
AMERICAN JOURNAL OF PATHOLOGY | Insulin promotes corneal nerve repair and wound healing in type 1 diabetic mice by enhancing Wnt/β-catenin signaling [Pubmed: 32858016] |
MEDIATORS OF INFLAMMATION | Aplysin Retards Pancreatic Necrosis and Inflammatory Responses in NOD Mice by Stabilizing Intestinal Barriers and Regulating Gut Microbial Composition [Pubmed: 32801992] |
EUROPEAN JOURNAL OF PHARMACOLOGY | Gut microbiota modulation as a promising therapy with metformin in rats with non-alcoholic steatohepatitis: Role of LPS/TLR4 and autophagy pathways [Pubmed: 32758573] |
ARCHIVES OF GYNECOLOGY AND OBSTETRICS | MiRNAs expression profiling of rat ovaries displaying PCOS with insulin resistance [Pubmed: 32757043] |
CHEMICO-BIOLOGICAL INTERACTIONS | Modulatory role of imatinib mesylate on pancreatic β-cells' secretory functions in an STZ rat model of diabetes mellitus [Pubmed: 32710900] |
Mol Med | Electroacupuncture alleviates polycystic ovary syndrome-like symptoms through improving insulin resistance, mitochondrial dysfunction, and endoplasmic?… [Pubmed: 32698821] |
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | Highly Branched RG-I Domain Enrichment are Indispensable for Pectin Mitigating Against High-Fat Diet-Induced Obesity [Pubmed: 32633953] |
Scientific Reports | Integrated metabolomics and gut microbiome to the effects and mechanisms of naoxintong capsule on type 2 diabetes in rats [Pubmed: 32616735] |
J Endocrinol Invest | Activation of central galanin receptor 2 mitigated insulin resistance in adipocytes of diabetic rats [Pubmed: 32588381] |
J Chem Neuroanat | The neuroprotective effect of simvastatin on the cerebellum of experimentally-induced diabetic rats through klotho upregulation: An immunohistochemical study [Pubmed: 32479899] |
Journal of Diabetes Research | Regulation and Mechanism of miR-518d through the PPARα-Mediated NF-κB Pathway in the Development of Gestational Diabetes Mellitus [Pubmed: 33123597] |
Journal of Cellular and Molecular Medicine | Cooperative effects of galanin and leptin on alleviation of insulin resistance in adipose tissue of diabetic rats [Pubmed: 32395890] |
BioMed Research International | Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress [Pubmed: 32685488] |
Frontiers in Pharmacology | MiR-185-5p Protects Against Angiogenesis in Polycystic Ovary Syndrome by Targeting VEGFA [Pubmed: 32760272] |
Iranian Journal of Basic Medical Sciences | Therapeutic effects of organic zinc on reproductive hormones, insulin resistance and mTOR expression as a novel component in a rat model of PCOS [] |
Nutrition & Metabolism | Fucoidan prevent murine autoimmune diabetes via suppression TLR4-signaling pathways, regulation DC/Treg induced immune tolerance and improving gut?… [] |
Journal of Functional Foods | Different source of commercial vegetable oils may regulate metabolic, inflammatory and redox status in healthy rats [] |
Comparative transcriptomic and metabolomic analysis reveals pectoralis highland adaptation across altitudinal songbirds [] | |
The effects of different intensity endurance and resistance exercise on diabetic-related blood profiles in impaired glucose tolerance mice [] | |
Cell Death & Disease | Phosphorylation of eIF2|á signaling pathway attenuates obesity-induced non-alcoholic fatty liver disease in an ER stress and autophagy-dependent manner [33318479] |
Ther Adv Chronic Dis | Empagliflozin improves chronic hypercortisolism-induced abnormal myocardial structure and cardiac function in mice [33294147] |
Physiol Behav | Time-restricted feeding attenuates gluconeogenic activity through inhibition of PGC-1|á expression and activity [33412190] |
PLoS Genetics | Physiological and genetic convergence supports hypoxia resistance in high-altitude songbirds [33370292] |
Mol Med Rep | N1?methylnicotinamide ameliorates insulin resistance in skeletal muscle of type 2 diabetic mice by activating the SIRT1/PGC?1|á signaling pathway [33576435] |
Arch Endocrinol Metab | Inverse association of plasma hydrogen sulfide levels with visceral fat area among Chinese young men: a cross-sectional study [33740335] |
Sci Rep | Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats [33833309] |
PeerJ | miR-155-5p upregulation ameliorates myocardial insulin resistance via mTOR signaling in chronic alcohol drinking rats [33868799] |
JOURNAL OF FOOD BIOCHEMISTRY | Identification of curcumin as a potential |á?\glucosidase and dipeptidyl?\peptidase 4 inhibitor: Molecular docking study, in vitro and in vivo biological evaluation [33817806] |
JOURNAL OF NUTRITIONAL BIOCHEMISTRY | Consumption of soybean or olive oil at recommended concentrations increased the intestinal microbiota diversity and insulin sensitivity and prevented fatty liver??- [33915261] |
Cell Metab | TMBIM1 is an inhibitor of adipogenesis and its depletion promotes adipocyte hyperplasia and improves obesity-related metabolic disease [34107313] |
Dapagliflozin: a Sodium-glucose Cotransporter 2 Inhibitor, Attenuates Angiotensin II-induced Cardiac Fibrotic Remodeling by Regulating TGF|?1/Smad ?- [34116674] | |
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY | Different Ratios of Corn and Coconut Oil Blends in High?\Fat Diets Influence Fat Deposition without Altering Metabolic Biomarkers in Male Rats [] |
Journal of Alzheimer's disease | DAla2-GIP-GLU-PAL Protects Against Cognitive Deficits and Pathology in APP/PS1 Mice by Inhibiting Neuroinflammation and Up-regulating cAMP/PKA/CREB??- [] |
International Journal of Diabetes in Developing Countries | Investigation of extracellular matrix genes associated with Alzheimer's disease in the hippocampus of experimental diabetic model rats [] |
researchsquare | Ficus deltoidea ameliorates biochemical, hormonal, and histomorphometric changes in letrozole-induced polycystic ovarian syndrome rats [] |
Cells Tissues Organs | Intermittent Fasting Attenuates High Fat Diet-Induced Cerebellar Changes in Rats: Involvement of TNFα, autophagy and oxidative stress [34551416] |
Experimental Biology and Medicine | Bee venom ameliorates cardiac dysfunction in diabetic hyperlipidemic rats [34550826] |
Phytomedicine | Baicalin protects against insulin resistance and metabolic dysfunction through activation of GALR2/GLUT4 signaling [34923235] |
Pharmacol Res | Lactobacillus acidophilus ameliorates obesity in mice through modulation of gut microbiota dysbiosis and intestinal permeability [34896249] |
Eat Weight Disord | Time-restricted feeding prevents metabolic diseases through the regulation of galanin/GALR1 expression in the hypothalamus of mice [34370270] |
Food Chem Toxicol | Combined treatment of sinapic acid and ellagic acid attenuates hyperglycemia in streptozotocin-induced diabetic rats [34329744] |
Eur J Pharmacol | Spexin ameliorates skeletal muscle insulin resistance through activation of GAL2 receptor [Pubmed:34973950] |
Surgical Endoscopy | The effect of bariatric surgery on the expression of Growth Differentiation Factor-15/Macrophage-Inhibitory Cytokine-1 (GDF-15/MIC-1) in rat [Pubmed:35029766] |
Arch Biochem Biophys | ATP6V1H deficiency impairs glucose tolerance by augmenting endoplasmic reticulum stress in high fat diet fed mice [Pubmed:34990584] |
Environ Sci Pollut Res Int | Environmental exposure to low-dose perfluorohexanesulfonate promotes obesity and non-alcoholic fatty liver disease in mice fed a high-fat diet [Pubmed:35217953] |
Naunyn Schmiedebergs Arch Pharmacol | Intragastric safflower yellow and its main component HSYA improve leptin sensitivity before body weight change in diet-induced obese mice [Pubmed:35201390] |
PLoS One | A cross-sectional comparative study on the effects of body mass index and exercise/sedentary on serum asprosin in male college students [Pubmed:35381008] |
Cell Death & Disease | Hepatic deficiency of selenoprotein S exacerbates hepatic steatosis and insulin resistance [Pubmed:35347118] |
Endocr J | A novel anti-obesity mechanism for liraglutide by improving adipose tissue leptin resistance in high-fat diet-fed obese mice [Pubmed:35705299] |
Antioxidants | Antioxidant Effect of Tyr-Ala Extracted from Zein on INS-1 Cells and Type 2 Diabetes High-Fat-Diet-Induced Mice [Pubmed:35740008] |
Mol Cell Endocrinol | Treatment with spexin mitigates diet-induced hepatic steatosis in vivo and in vitro through activation of galanin receptor 2 [Pubmed:35654225] |
Biofabrication | Dual-core coaxial bioprinting of double-channel constructs with a potential for perfusion and interaction of cells [Pubmed:35616388] |
Ecotoxicology and Environmental Safety | Constitutional delay of growth and puberty in female mice is induced by circadian rhythm disruption in utero [Pubmed:35679725] |
SAUDI J BIOL SCI | Aqueous Ajwa dates seeds extract improves memory impairment in type-2 diabetes mellitus rats by reducing blood glucose levels and enhancing brain?… [Pubmed:35531250] |
Saudi Pharmaceutical Journal | Sukkari dates seed improves type-2 diabetes mellitus-induced memory impairment by reducing blood glucose levels and enhancing brain cholinergic?… [Pubmed:35812141] |
Turk J Diab Obes | An Investigation of the Effect of Curcumin on Fructose-Induced Metabolic Syndrome Rat Models [] |
Journal of Drug Delivery Science and Technology | Miglitol-PMO nanoparticles, an alpha-glucosidase inhibitor, has prolonged action on blood glucose level in diabetic rats [] |
Genes & Diseases | Prolonged control of insulin-dependent diabetes via intramuscular expression of plasmid-encoded single-strand insulin analogue [] |
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