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細(xì)胞毒性T-淋巴細(xì)胞關(guān)聯(lián)抗原4(CTLA4)重組抗體

Recombinant Antibody to Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4)

CD152; CELIAC3; CTLA4; GSE; IDDM12; Celiac Disease 3; Cytotoxic T-lymphocyte protein 4

  • 細(xì)胞毒性T-淋巴細(xì)胞關(guān)聯(lián)抗原4(CTLA4)重組抗體 產(chǎn)品包裝(模擬)
  • 細(xì)胞毒性T-淋巴細(xì)胞關(guān)聯(lián)抗原4(CTLA4)重組抗體 產(chǎn)品包裝(模擬)
  • 細(xì)胞毒性T-淋巴細(xì)胞關(guān)聯(lián)抗原4(CTLA4)重組抗體 Briefly, biotin labelled CTLA4 Nano-antibody were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to rh CTLA4-coated microtiter wells and incubated for 1h at 37℃. Wells were washed 3 times with PBST and incubated for 0.5 h at 37℃ with SA labelled HRP. After incubation with SA labelled HRP, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450/630 nm immediately. The binding activity of rh CTLA4 and CTLA4 Nano-antibody was shown in Figure 1, and the ED50 for this effect is 0.06607 ug/ml.
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對CTLA4的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識別CTLA4。

用法

免疫印跡:0.2-2μg/mL;1:500-5000
免疫組織化學(xué):5-20μg/mL;1:50-200
免疫細(xì)胞化學(xué):5-20μg/mL;1:50-200
最佳稀釋倍數(shù)最終由用戶決定。

儲存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲,損失率低于5%。

贈品

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編號 適用物種:Homo sapiens (Human,人) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPB230Hu01 細(xì)胞毒性T-淋巴細(xì)胞關(guān)聯(lián)抗原4(CTLA4)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB230Hu03 細(xì)胞毒性T-淋巴細(xì)胞關(guān)聯(lián)抗原4(CTLA4)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB230Hu02 細(xì)胞毒性T-淋巴細(xì)胞關(guān)聯(lián)抗原4(CTLA4)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB230Hu04 細(xì)胞毒性T-淋巴細(xì)胞關(guān)聯(lián)抗原4(CTLA4)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB230Hu02 細(xì)胞毒性T-淋巴細(xì)胞關(guān)聯(lián)抗原4(CTLA4)多克隆抗體 WB; IHC; ICC; IP.
PAB230Hu01 細(xì)胞毒性T-淋巴細(xì)胞關(guān)聯(lián)抗原4(CTLA4)多克隆抗體 WB; IHC; ICC; IP.
FAB230Hu01 抗細(xì)胞毒性T-淋巴細(xì)胞關(guān)聯(lián)抗原4(CTLA4)多克隆抗體 Flow cytometry.
FAB230Hu41 抗細(xì)胞毒性T-淋巴細(xì)胞關(guān)聯(lián)抗原4(CTLA4)多克隆抗體(藻紅素標(biāo)記) Flow cytometry.
FAB230Hu51 抗細(xì)胞毒性T-淋巴細(xì)胞關(guān)聯(lián)抗原4(CTLA4)多克隆抗體(別藻藍(lán)蛋白標(biāo)記) Flow cytometry.
FAB230Hu81 抗細(xì)胞毒性T-淋巴細(xì)胞關(guān)聯(lián)抗原4(CTLA4)多克隆抗體(異硫氰酸熒光素標(biāo)記) Flow cytometry.
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MAB230Hu23 細(xì)胞毒性T-淋巴細(xì)胞關(guān)聯(lián)抗原4(CTLA4)單克隆抗體 WB; IHC; ICC; IP.
RAB230Hu71 細(xì)胞毒性T-淋巴細(xì)胞關(guān)聯(lián)抗原4(CTLA4)重組抗體 WB; IF; ICC; IHC; IP; FCM.
MAB230Hu22 細(xì)胞毒性T-淋巴細(xì)胞關(guān)聯(lián)抗原4(CTLA4)單克隆抗體 WB; IHC; ICC; IP.
MAB230Hu28 細(xì)胞毒性T-淋巴細(xì)胞關(guān)聯(lián)抗原4(CTLA4)單克隆抗體 WB; IHC; ICC; IP.
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MAB230Hu24 細(xì)胞毒性T-淋巴細(xì)胞關(guān)聯(lián)抗原4(CTLA4)單克隆抗體 WB; IHC; ICC; IP.
MAB230Hu25 細(xì)胞毒性T-淋巴細(xì)胞關(guān)聯(lián)抗原4(CTLA4)單克隆抗體 WB; IHC; ICC; IP.
MAB230Hu26 細(xì)胞毒性T-淋巴細(xì)胞關(guān)聯(lián)抗原4(CTLA4)單克隆抗體 WB; IHC; ICC; IP.
MAB230Hu27 細(xì)胞毒性T-淋巴細(xì)胞關(guān)聯(lián)抗原4(CTLA4)單克隆抗體 WB; IHC; ICC; IP.
SEB230Hu 細(xì)胞毒性T-淋巴細(xì)胞關(guān)聯(lián)抗原4(CTLA4)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
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參考文獻(xiàn)

雜志 參考文獻(xiàn)
Int Immunopharmacol Circulating CTLA-4 levels and CTLA4 polymorphisms associate with disease condition and progression and hepatocellular carcinoma patients' survival in chronic … [Pubmed: 32163858]
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