神經(jīng)生長(zhǎng)因子(NGF)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Nerve Growth Factor (NGF)
NGFB; Beta-NGF; HSAN5; NGF-B; Beta-Nerve Growth Factor
- 編號(hào)KSA105Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍15.6-1,000pg/mL
- 可適靈敏度6.2pg/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 6048 ¥ 10080 ¥ 18144 ¥ 35280 ¥ 60480
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)神經(jīng)生長(zhǎng)因子(NGF)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA105Hu02 | 神經(jīng)生長(zhǎng)因子(NGF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA105Hu61 | 神經(jīng)生長(zhǎng)因子(NGF)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA105Hu01 | 神經(jīng)生長(zhǎng)因子(NGF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA105Hu01 | 神經(jīng)生長(zhǎng)因子(NGF)活性蛋白 | Cell?culture;?Activity?Assays. |
| EPA105Hu61 | 神經(jīng)生長(zhǎng)因子(NGF)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA105Hu02 | 神經(jīng)生長(zhǎng)因子(NGF)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA105Hu01 | 神經(jīng)生長(zhǎng)因子(NGF)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA105Hu06 | 神經(jīng)生長(zhǎng)因子(NGF)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA105Hu71 | 神經(jīng)生長(zhǎng)因子(NGF)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAA105Hu25 | 神經(jīng)生長(zhǎng)因子(NGF)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA105Hu27 | 神經(jīng)生長(zhǎng)因子(NGF)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA105Hu29 | 神經(jīng)生長(zhǎng)因子(NGF)單克隆抗體 | IHC |
| MAA105Hu21 | 神經(jīng)生長(zhǎng)因子(NGF)單克隆抗體 | WB |
| MAA105Hu22 | 神經(jīng)生長(zhǎng)因子(NGF)單克隆抗體 | WB,ICC/IF |
| MAA105Hu24 | 神經(jīng)生長(zhǎng)因子(NGF)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA105Hu23 | 神經(jīng)生長(zhǎng)因子(NGF)單克隆抗體 | WB |
| FAA105Hu02 | 抗神經(jīng)生長(zhǎng)因子(NGF)單克隆抗體 | Flow cytometry. |
| SEA105Hu | 神經(jīng)生長(zhǎng)因子(NGF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA105Hu | 神經(jīng)生長(zhǎng)因子(NGF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA105Hu | 神經(jīng)生長(zhǎng)因子(NGF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA105Hu | 神經(jīng)生長(zhǎng)因子(NGF)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA105Hu01 | 神經(jīng)生長(zhǎng)因子(NGF)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| The Tohoku Journal of Experimental Medicine | Low-intensity aerobic exercise mitigates exercise-induced bronchoconstriction by improving the function of adrenal medullary chromaffin cells in asthmatic rats [Pubmed:25212248] |
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| Yonsei Medical Journal | NGF and HB-EGF: Potential Biomarkers that Reflect the Effects of Fesoterodine in Patients with Overactive Bladder Syndrome [Pubmed:25510766] |
| Molecular and Cellular Endocrinology | High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 to increase NGF secretion in a rat Schwann cell line RSC96 [Pubmed:25614971] |
| Mol Cell Endocrinol | High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 [PubMed: 25614971] |
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| Genet Mol Res | Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells [Pubmed:27323064] |
| The Journal of Maternal-Fetal & Neonatal Medicine | Differential expression of cord blood neurotrophins in gestational diabetes: the impact of fetal growth abnormalities [pubmed:28081639] |
| IOS Press Content Library | An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … [articles:journal-of-alzheimers-disease] |
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| MOLECULAR NUTRITION & FOOD RESEARCH | Hyperlipidemia Down‐Regulate Brain Antioxidant Defense Enzymes and Neurotrophins in Rats: Assessment of the Modulatory Potential of EPA+ DHA and?… [Pubmed: 32918393] |
| Neuropharmacology | Impaired LTD-like motor cortical plasticity in female patients with major depression disorder [Pubmed: 32791084] |
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| Medical Research Journal | Changes of substance P, NGF and CGRP salivary levels among patients undergoing physical therapy [] |
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| Cell Biochem Funct | CNTs‐CaP/chitosan‐coated AZ91D magnesium alloy extract promoted rat dorsal root ganglia neuron growth via activating ERK signalling pathway [34296452] |
| Carbohydrate Polymers | Gene delivery of chitosan-graft-polyethyleneimine vectors loaded on scaffolds for nerve regeneration [Pubmed:35550777] |
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