神經(jīng)生長因子(NGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)
High Sensitive ELISA Kit for Nerve Growth Factor (NGF)
NGFB; Beta-NGF; HSAN5; NGF-B; Beta-Nerve Growth Factor
- 編號HEA105Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍3.12-200pg/mL
- 靈敏度最小可檢測劑量小于等于1.22pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 2520 ¥ 3600 ¥ 16200 ¥ 30600 ¥ 252000
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測神經(jīng)生長因子(NGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的神經(jīng)生長因子(NGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 96-104 | 101 |
| EDTA plasma(n=5) | 98-105 | 102 |
| heparin plasma(n=5) | 99-105 | 102 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的神經(jīng)生長因子(NGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中神經(jīng)生長因子(NGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-93% | 83-94% | 93-101% | 94-101% |
| EDTA plasma(n=5) | 86-96% | 80-104% | 79-104% | 93-101% |
| heparin plasma(n=5) | 94-102% | 78-95% | 87-98% | 87-96% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將神經(jīng)生長因子(NGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的神經(jīng)生長因子(NGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的神經(jīng)生長因子(NGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的神經(jīng)生長因子(NGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA105Hu02 | 神經(jīng)生長因子(NGF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA105Hu61 | 神經(jīng)生長因子(NGF)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA105Hu01 | 神經(jīng)生長因子(NGF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA105Hu01 | 神經(jīng)生長因子(NGF)活性蛋白 | Cell?culture;?Activity?Assays. |
| EPA105Hu61 | 神經(jīng)生長因子(NGF)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA105Hu02 | 神經(jīng)生長因子(NGF)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA105Hu01 | 神經(jīng)生長因子(NGF)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA105Hu06 | 神經(jīng)生長因子(NGF)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA105Hu71 | 神經(jīng)生長因子(NGF)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAA105Hu25 | 神經(jīng)生長因子(NGF)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA105Hu27 | 神經(jīng)生長因子(NGF)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA105Hu29 | 神經(jīng)生長因子(NGF)單克隆抗體 | IHC |
| MAA105Hu21 | 神經(jīng)生長因子(NGF)單克隆抗體 | WB |
| MAA105Hu22 | 神經(jīng)生長因子(NGF)單克隆抗體 | WB,ICC/IF |
| MAA105Hu24 | 神經(jīng)生長因子(NGF)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA105Hu23 | 神經(jīng)生長因子(NGF)單克隆抗體 | WB |
| FAA105Hu02 | 抗神經(jīng)生長因子(NGF)單克隆抗體 | Flow cytometry. |
| SEA105Hu | 神經(jīng)生長因子(NGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA105Hu | 神經(jīng)生長因子(NGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA105Hu | 神經(jīng)生長因子(NGF)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA105Hu | 神經(jīng)生長因子(NGF)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA105Hu01 | 神經(jīng)生長因子(NGF)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| The Tohoku Journal of Experimental Medicine | Low-intensity aerobic exercise mitigates exercise-induced bronchoconstriction by improving the function of adrenal medullary chromaffin cells in asthmatic rats [Pubmed:25212248] |
| International Journal of Pharmaceutics | Neurotrophic and antioxidant effects of silymarin comparable to 4-methylcatechol in protection against gentamicin-induced ototoxicity in guinea pigs [Pubmed:25445537] |
| Yonsei Medical Journal | NGF and HB-EGF: Potential Biomarkers that Reflect the Effects of Fesoterodine in Patients with Overactive Bladder Syndrome [Pubmed:25510766] |
| Molecular and Cellular Endocrinology | High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 to increase NGF secretion in a rat Schwann cell line RSC96 [Pubmed:25614971] |
| Mol Cell Endocrinol | High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 [PubMed: 25614971] |
| Neurosciences (Riyadh) | Telmisartan inhibits hyperalgesia and inflammatory progression in a diabetic neuropathic pain model of Wistar rats. [PubMed: 25864063] |
| Neurotoxicity Research | Pro-apoptotic Action of Corticosterone in Hippocampal Organotypic Cultures [Pubmed:27189478] |
| Psychoneuroendocrinology | Chronic mild stress influences nerve growth factor through a matrix metalloproteinase-dependent mechanism [Pubmed:26771945] |
| Genet Mol Res | Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells [Pubmed:27323064] |
| The Journal of Maternal-Fetal & Neonatal Medicine | Differential expression of cord blood neurotrophins in gestational diabetes: the impact of fetal growth abnormalities [pubmed:28081639] |
| IOS Press Content Library | An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … [articles:journal-of-alzheimers-disease] |
| biblioteca.uva | Evaluación de la secreción de factores neurotróficos por las células MSV en cocultivo con neurorretinas porcinas, en función de la dosis celular y del tiempo de cultivo [10324/27440] |
| Progress in Neuro-Psychopharmacology & Biological Psychiatry | Antidepressant drugs for beta amyloid-induced depression: A new standpoint? [pubmed:28499898] |
| Osteoarthritis and Cartilage | Mechanical loading of intervertebral disc modulates microglia proliferation, activation, and chemotaxis [Pubmed:29723636] |
| Stem?Cell?Research?&?Therapy | Autophagy is required for human umbilical cord mesenchymal stem cells to improve spatial working memory in APP/PS1 transgenic mouse model [Pubmed:29335016] |
| Pharmacological?Research? | N-3 PUFA diet enrichment prevents amyloid beta-induced depressive-like phenotype [Pubmed:29203442] |
| Experimental Neurology | Preterm umbilical cord blood derived mesenchymal stem/stromal cells protect preterm white matter brain development against hypoxia-ischemia [Pubmed:30012511] |
| Biochemical Pharmacology | Effects of n-3 PUFA enriched and n-3 PUFA deficient diets in na?ve and Aβ-treated female rats [Pubmed:30028991] |
| Experimental Dermatology | 3‐‐Laurylglyceryl ascorbate improves the development of sensitive skin through the reduction of oxidative stress [Pubmed: 30698879] |
| BioMed Research International | Administration of Repetitive Transcranial Magnetic Stimulation Attenuates Aβ1-42-Induced Alzheimer's Disease in Mice by Activating β-Catenin Signaling [] |
| International Journal of Ophthalmology | A novel xeno-free culture system for human retinal pigment epithelium cells [Pubmed: 31024807] |
| Experimental and Therapeutic Medicine | Involvement of neurotrophic signaling in doxorubicin?induced cardiotoxicity [Pubmed: 32010279] |
| Open Access Macedonian Journal of Medical Sciences | Inhibition of Receptor for Advanced Glycation End Products as New Promising Strategy Treatment in Diabetic Retinopathy [Pubmed: 32165929] |
| MOLECULAR NUTRITION & FOOD RESEARCH | Hyperlipidemia Down‐Regulate Brain Antioxidant Defense Enzymes and Neurotrophins in Rats: Assessment of the Modulatory Potential of EPA+ DHA and?… [Pubmed: 32918393] |
| Neuropharmacology | Impaired LTD-like motor cortical plasticity in female patients with major depression disorder [Pubmed: 32791084] |
| COLLOIDS AND SURFACES B-BIOINTERFACES | Effects of gold nanoparticles administration through behavioral and oxidative parameters in animal model of parkinson's disease [Pubmed: 32777662] |
| 博士論文 | Neurobiological determinants of depressive-like symptoms in rodents [] |
| Medical Research Journal | Changes of substance P, NGF and CGRP salivary levels among patients undergoing physical therapy [] |
| Journal of the Taiwan Institute of Chemical Engineers | Multiple-component dual-phase solid lipid nanoparticles with conjugated transferrin for formulating antioxidants and nerve growth factor against neuronal apoptosis [] |
| EUROPEAN CHILD & ADOLESCENT PSYCHIATRY | Increased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free??- [33389158] |
| DRUG DEVELOPMENT RESEARCH | Neuroprotective effect of Na+/H+?exchangers isoform?\1 inactivation against 6?\hydroxydopamine?\induced mitochondrial dysfunction and neuronal apoptosis in Parkinson's disease models [33538000] |
| BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | Sea urchin gangliosides exhibit neuritogenic effects in neuronal PC12 cells via TrkA- and TrkB-related pathways [33589896] |
| Neuropsychiatr Dis Treat | Repetitive Transcranial Magnetic Stimulation Improves Mild Cognitive Impairment Associated with Alzheimer's Disease in Mice by Modulating the miR-567?… [34239303] |
| Pharmaceuticals (Basel) | Carissa macrocarpa Leaves Polar Fraction Ameliorates Doxorubicin-Induced Neurotoxicity in Rats via Downregulating the Oxidative Stress and Inflammatory Markers [34959705] |
| Cell Biochem Funct | CNTs‐CaP/chitosan‐coated AZ91D magnesium alloy extract promoted rat dorsal root ganglia neuron growth via activating ERK signalling pathway [34296452] |
| Carbohydrate Polymers | Gene delivery of chitosan-graft-polyethyleneimine vectors loaded on scaffolds for nerve regeneration [Pubmed:35550777] |
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