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泛素羧基端酯酶L1(UCHL1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)

PARK5; PGP9.5; Uch-L1; Neuron cytoplasmic protein 9.5; Ubiquitin thioesterase L1; Ubiquitin carboxyl-terminal hydrolase isozyme L1

  • 泛素羧基端酯酶L1(UCHL1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 泛素羧基端酯酶L1(UCHL1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

試劑盒中的抗體對泛素羧基端酯酶L1(UCHL1)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

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編號 適用物種:Homo sapiens (Human,人) 應用(僅供研究使用,不用于臨床診斷!)
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AEG945Hu 抗泛素羧基端酯酶L1抗體(Anti-UCHL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antibody Detection.
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參考文獻

雜志 參考文獻
J Neurol Sci Changes of ubiquitin C-terminal hydrolase-L1 levels in serum and urine of patients with white matter lesions [PubMed: 26232084]
Journal of the Neurological Sciences Increased plasma UCH-L1 after aneurysmal subarachnoid hemorrhage is associated with unfavorable neurological outcome [Pubmed:26810533]
Ulutas Medical Journal Serum Carnosine Dipeptidase 1 and Ubiquitin C - Terminal Hydrolase L1 as Markers of Brain Damage in Patients after Carotid Endarterectomy [mnstemps:135]
Scientific Reports Activation of hepatic stellate cells by the ubiquitin C-terminal hydrolase 1 protein secreted from hepatitis C virus-infected hepatocytes [pubmed:28667290]
Intensive?Care?Medicine Relationships between markers of neurologic and endothelial injury during critical illness and long-term cognitive impairment and disability [Pubmed:29523900]
Earth and Environmental Science Serum concentration of ubiquitin c-terminal hydrolase-L1 in detecting severity of traumatic brain injury [article:10.1088]
Peripheral blood biomarkers in aneurysmal subarachnoid hemorrhage [ISBN:978-952-03-0750-9]
Journal of Critical Care Association of neuronal repair biomarkers with delirium among survivors of critical illness [Pubmed: 31896448]
Brain Sciences BDNF and IL-8, But Not UCHL-1 and IL-11, Are Markers of Brain Injury in Children Caused by Mild Head Trauma [Pubmed: 32987792]
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