泛素羧基端酯酶L1(UCHL1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)
PARK5; PGP9.5; Uch-L1; Neuron cytoplasmic protein 9.5; Ubiquitin thioesterase L1; Ubiquitin carboxyl-terminal hydrolase isozyme L1
- 編號KSG945Hu01
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍0.78-50ng/mL
- 可適靈敏度0.27ng/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
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特異性
試劑盒中的抗體對泛素羧基端酯酶L1(UCHL1)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務
相關產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應用(僅供研究使用,不用于臨床診斷!) |
RPG945Hu01 | 泛素羧基端酯酶L1(UCHL1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAG945Hu01 | 泛素羧基端酯酶L1(UCHL1)多克隆抗體 | WB; IHC; ICC; IP. |
FAG945Hu01 | 抗泛素羧基端酯酶L1(UCHL1)多克隆抗體 | Flow cytometry. |
FAG945Hu41 | 抗泛素羧基端酯酶L1(UCHL1)多克隆抗體(藻紅素標記) | Flow cytometry. |
FAG945Hu51 | 抗泛素羧基端酯酶L1(UCHL1)多克隆抗體(別藻藍蛋白標記) | Flow cytometry. |
FAG945Hu81 | 抗泛素羧基端酯酶L1(UCHL1)多克隆抗體(異硫氰酸熒光素標記) | Flow cytometry. |
MAG945Hu21 | 泛素羧基端酯酶L1(UCHL1)單克隆抗體 | WB; IHC; ICC; IP. |
FAG945Hu02 | 抗泛素羧基端酯酶L1(UCHL1)單克隆抗體 | Flow cytometry. |
FAG945Hu42 | 抗泛素羧基端酯酶L1(UCHL1)單克隆抗體(藻紅素標記) | Flow cytometry. |
FAG945Hu52 | 抗泛素羧基端酯酶L1(UCHL1)單克隆抗體(別藻藍蛋白標記) | Flow cytometry. |
FAG945Hu82 | 抗泛素羧基端酯酶L1(UCHL1)單克隆抗體(異硫氰酸熒光素標記) | Flow cytometry. |
SEG945Hu | 泛素羧基端酯酶L1(UCHL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
HEG945Hu | 泛素羧基端酯酶L1(UCHL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
AEG945Hu | 抗泛素羧基端酯酶L1抗體(Anti-UCHL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
LMG945Hu | 泛素羧基端酯酶L1(UCHL1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSG945Hu01 | 泛素羧基端酯酶L1(UCHL1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
J Neurol Sci | Changes of ubiquitin C-terminal hydrolase-L1 levels in serum and urine of patients with white matter lesions [PubMed: 26232084] |
Journal of the Neurological Sciences | Increased plasma UCH-L1 after aneurysmal subarachnoid hemorrhage is associated with unfavorable neurological outcome [Pubmed:26810533] |
Ulutas Medical Journal | Serum Carnosine Dipeptidase 1 and Ubiquitin C - Terminal Hydrolase L1 as Markers of Brain Damage in Patients after Carotid Endarterectomy [mnstemps:135] |
Scientific Reports | Activation of hepatic stellate cells by the ubiquitin C-terminal hydrolase 1 protein secreted from hepatitis C virus-infected hepatocytes [pubmed:28667290] |
Intensive?Care?Medicine | Relationships between markers of neurologic and endothelial injury during critical illness and long-term cognitive impairment and disability [Pubmed:29523900] |
Earth and Environmental Science | Serum concentration of ubiquitin c-terminal hydrolase-L1 in detecting severity of traumatic brain injury [article:10.1088] |
Peripheral blood biomarkers in aneurysmal subarachnoid hemorrhage [ISBN:978-952-03-0750-9] | |
Journal of Critical Care | Association of neuronal repair biomarkers with delirium among survivors of critical illness [Pubmed: 31896448] |
Brain Sciences | BDNF and IL-8, But Not UCHL-1 and IL-11, Are Markers of Brain Injury in Children Caused by Mild Head Trauma [Pubmed: 32987792] |
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