泛素羧基端酯酶L1(UCHL1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)
High Sensitive ELISA Kit for Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)
PARK5; PGP9.5; Uch-L1; Neuron cytoplasmic protein 9.5; Ubiquitin thioesterase L1; Ubiquitin carboxyl-terminal hydrolase isozyme L1
- 編號(hào)HEG945Hu
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長(zhǎng)3h
- 檢測(cè)范圍0.156-10ng/mL
- 靈敏度最小可檢測(cè)劑量小于等于0.057ng/mL.
- 樣本類型tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
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特異性
本試劑盒用于檢測(cè)泛素羧基端酯酶L1(UCHL1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型),經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制,不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè),因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次,分別計(jì)算不同濃度樣本的平均值及SD值。
批間差:選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定,每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次,分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%
穩(wěn)定性
經(jīng)測(cè)定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響,實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致,尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備;
2. 加樣(標(biāo)準(zhǔn)品及樣本)100µL,37°C孵育1小時(shí);
3. 吸棄,加檢測(cè)溶液A100µL,37°C孵育1小時(shí);
4. 洗板3次;
5. 加檢測(cè)溶液B100µL,37°C孵育30分鐘;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分鐘;
8. 加終止液50µL,立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將泛素羧基端酯酶L1(UCHL1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本,其中的泛素羧基端酯酶L1(UCHL1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)與連接于固相載體上的抗體結(jié)合,然后加入生物素化的泛素羧基端酯酶L1(UCHL1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)抗體,將未結(jié)合的生物素化抗體洗凈后,加入HRP標(biāo)記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過(guò)氧化物酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的泛素羧基端酯酶L1(UCHL1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(O.D.值),計(jì)算樣品濃度。
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
編號(hào) | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPG945Hu01 | 泛素羧基端酯酶L1(UCHL1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAG945Hu01 | 泛素羧基端酯酶L1(UCHL1)多克隆抗體 | WB; IHC; ICC; IP. |
FAG945Hu01 | 抗泛素羧基端酯酶L1(UCHL1)多克隆抗體 | Flow cytometry. |
FAG945Hu41 | 抗泛素羧基端酯酶L1(UCHL1)多克隆抗體(藻紅素標(biāo)記) | Flow cytometry. |
FAG945Hu51 | 抗泛素羧基端酯酶L1(UCHL1)多克隆抗體(別藻藍(lán)蛋白標(biāo)記) | Flow cytometry. |
FAG945Hu81 | 抗泛素羧基端酯酶L1(UCHL1)多克隆抗體(異硫氰酸熒光素標(biāo)記) | Flow cytometry. |
MAG945Hu21 | 泛素羧基端酯酶L1(UCHL1)單克隆抗體 | WB; IHC; ICC; IP. |
FAG945Hu02 | 抗泛素羧基端酯酶L1(UCHL1)單克隆抗體 | Flow cytometry. |
FAG945Hu42 | 抗泛素羧基端酯酶L1(UCHL1)單克隆抗體(藻紅素標(biāo)記) | Flow cytometry. |
FAG945Hu52 | 抗泛素羧基端酯酶L1(UCHL1)單克隆抗體(別藻藍(lán)蛋白標(biāo)記) | Flow cytometry. |
FAG945Hu82 | 抗泛素羧基端酯酶L1(UCHL1)單克隆抗體(異硫氰酸熒光素標(biāo)記) | Flow cytometry. |
SEG945Hu | 泛素羧基端酯酶L1(UCHL1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
HEG945Hu | 泛素羧基端酯酶L1(UCHL1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
AEG945Hu | 抗泛素羧基端酯酶L1抗體(Anti-UCHL1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
LMG945Hu | 泛素羧基端酯酶L1(UCHL1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSG945Hu01 | 泛素羧基端酯酶L1(UCHL1)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
J Neurol Sci | Changes of ubiquitin C-terminal hydrolase-L1 levels in serum and urine of patients with white matter lesions [PubMed: 26232084] |
Journal of the Neurological Sciences | Increased plasma UCH-L1 after aneurysmal subarachnoid hemorrhage is associated with unfavorable neurological outcome [Pubmed:26810533] |
Ulutas Medical Journal | Serum Carnosine Dipeptidase 1 and Ubiquitin C - Terminal Hydrolase L1 as Markers of Brain Damage in Patients after Carotid Endarterectomy [mnstemps:135] |
Scientific Reports | Activation of hepatic stellate cells by the ubiquitin C-terminal hydrolase 1 protein secreted from hepatitis C virus-infected hepatocytes [pubmed:28667290] |
Intensive?Care?Medicine | Relationships between markers of neurologic and endothelial injury during critical illness and long-term cognitive impairment and disability [Pubmed:29523900] |
Earth and Environmental Science | Serum concentration of ubiquitin c-terminal hydrolase-L1 in detecting severity of traumatic brain injury [article:10.1088] |
Peripheral blood biomarkers in aneurysmal subarachnoid hemorrhage [ISBN:978-952-03-0750-9] | |
Journal of Critical Care | Association of neuronal repair biomarkers with delirium among survivors of critical illness [Pubmed: 31896448] |
Brain Sciences | BDNF and IL-8, But Not UCHL-1 and IL-11, Are Markers of Brain Injury in Children Caused by Mild Head Trauma [Pubmed: 32987792] |