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補(bǔ)體成分3a(C3a)活性蛋白

Active Complement Component 3a (C3a)

  • 補(bǔ)體成分3a(C3a)活性蛋白 產(chǎn)品包裝(模擬)
  • 補(bǔ)體成分3a(C3a)活性蛋白 產(chǎn)品包裝(模擬)
  • APA387Hu01.jpg Figure. SDS-PAGE
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

Complement Component 3a (C3a) is a component of the complement system, this protein is also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. It's been identified that C3A can interact with Complement Factor I (CFI), thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human C3a and recombinant human CFI. Briefly, C3a was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CFI-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-C3a pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human C3a and recombinant human CFI was shown in Figure 1, the EC50?for this effect is 0.03 ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲(chǔ)存

避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

相關(guān)產(chǎn)品

編號(hào) 適用物種：Homo sapiens (Human，人) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA387Hu01 補(bǔ)體成分3a(C3a)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA387Hu01 補(bǔ)體成分3a(C3a)活性蛋白 Cell?culture;?Activity?Assays.
PAA387Hu01 補(bǔ)體成分3a(C3a)多克隆抗體 WB; IHC; ICC; IP.
MAA387Hu27 補(bǔ)體成分3a(C3a)單克隆抗體 WB; IHC; ICC; IP.
MAA387Hu21 補(bǔ)體成分3a(C3a)單克隆抗體 WB; IHC; ICC; IP.
MAA387Hu22 補(bǔ)體成分3a(C3a)單克隆抗體 WB; IHC; ICC; IP.
MAA387Hu23 補(bǔ)體成分3a(C3a)單克隆抗體 WB; IHC; ICC; IP.
MAA387Hu24 補(bǔ)體成分3a(C3a)單克隆抗體 WB; IHC; ICC; IP.
MAA387Hu29 補(bǔ)體成分3a(C3a)單克隆抗體 WB; IHC; ICC; IP.
MAA387Hu25 補(bǔ)體成分3a(C3a)單克隆抗體 WB; IHC; ICC; IP.
SEA387Hu 補(bǔ)體成分3a(C3a)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
WEA387Hu 補(bǔ)體成分3a(C3a)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,寬范圍) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA387Hu 補(bǔ)體成分3a(C3a)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA387Hu 補(bǔ)體成分3a(C3a)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA387Hu01 補(bǔ)體成分3a(C3a)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志 參考文獻(xiàn)
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Plos One Alternative Pathway Inhibition by Exogenous Factor H Fails to Attenuate Inflammation and Vascular Leakage in Experimental Pneumococcal Sepsis in Mice [Pubmed:26872035]
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