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補體成分3a(C3a)檢測試劑盒(化學(xué)發(fā)光免疫分析法)

CLIA Kit for Complement Component 3a (C3a)

  • 補體成分3a(C3a)檢測試劑盒(化學(xué)發(fā)光免疫分析法) 產(chǎn)品包裝(模擬)
  • 補體成分3a(C3a)檢測試劑盒(化學(xué)發(fā)光免疫分析法) 產(chǎn)品包裝(模擬)
  • 補體成分3a(C3a)檢測試劑盒(化學(xué)發(fā)光免疫分析法) 實驗結(jié)果圖
  • SCA387Hu.jpg 標準曲線圖
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒用于檢測補體成分3a(C3a)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的補體成分3a(C3a)（加標樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 87-96 90
EDTA plasma(n=5) 91-105 97
heparin plasma(n=5) 88-96 92

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的補體成分3a(C3a)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中補體成分3a(C3a)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 95-103% 96-105% 92-101% 80-96%
EDTA plasma(n=5) 90-97% 79-93% 91-99% 81-90%
heparin plasma(n=5) 79-94% 80-99% 88-96% 89-103%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100μL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加底物100µL，37°C孵育10分鐘；
8. 讀數(shù)。

實驗原理

將補體成分3a(C3a)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的補體成分3a(C3a)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的補體成分3a(C3a)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入底物。加入底物后會產(chǎn)生輝光型光信號。發(fā)射光強度和樣品中的補體成分3a(C3a)呈正相關(guān)。用化學(xué)發(fā)光儀測定相對光單位（RLU），計算樣品濃度。

相關(guān)產(chǎn)品

編號 適用物種：Homo sapiens (Human，人) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA387Hu01 補體成分3a(C3a)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA387Hu01 補體成分3a(C3a)活性蛋白 Cell?culture;?Activity?Assays.
PAA387Hu01 補體成分3a(C3a)多克隆抗體 WB; IHC; ICC; IP.
MAA387Hu27 補體成分3a(C3a)單克隆抗體 WB; IHC; ICC; IP.
MAA387Hu21 補體成分3a(C3a)單克隆抗體 WB; IHC; ICC; IP.
MAA387Hu22 補體成分3a(C3a)單克隆抗體 WB; IHC; ICC; IP.
MAA387Hu23 補體成分3a(C3a)單克隆抗體 WB; IHC; ICC; IP.
MAA387Hu24 補體成分3a(C3a)單克隆抗體 WB; IHC; ICC; IP.
MAA387Hu29 補體成分3a(C3a)單克隆抗體 WB; IHC; ICC; IP.
MAA387Hu25 補體成分3a(C3a)單克隆抗體 WB; IHC; ICC; IP.
SEA387Hu 補體成分3a(C3a)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
WEA387Hu 補體成分3a(C3a)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA387Hu 補體成分3a(C3a)檢測試劑盒(化學(xué)發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA387Hu 補體成分3a(C3a)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA387Hu01 補體成分3a(C3a)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志 參考文獻
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Artificial?Cells?Nanomedicine and Biotechnology Journal Long term safety and immunological effects of a nanobiotherapeutic, bovine poly-[hemoglobin-catalase-superoxide dismutase-carbonic anhydrase], after four weekly?… [Pubmed:29873524]
Cytotherapy Mesenchymal stromal cell secretory factors induce sustained improvements in islet function pre-and post-transplantation [Doi: 10.1016/j.jcyt.2018.07.007]
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Materials Science and Engineering: C Hemocompatibility of biodegradable Zn-0.8 wt%(Cu, Mn, Li) alloys []
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EXPERIMENTAL EYE RESEARCH Increased ratios of complement factors C3a to C3 in aqueous humor and serum mark glaucoma progression [33493474]
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