無翅型MMTV整合位點家族成員5A(WNT5A)多克隆抗體
Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)
- 編號PAP549Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPP549Mu01-無翅型MMTV整合位點家族成員5A(WNT5A)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度500μg/mL
- 且適物種Homo sapiens (Human，人), Rattus norvegicus (Rat，大鼠), Cavia (Guinea pig ，豚鼠), Canis familiaris; Canine (Dog，犬), Equus caballus; Equine (Horse，馬)
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 713 ¥ 1663 ¥ 2376 ¥ 5940 ¥ 23760
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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IHC圖
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Western Blot; Sample: Lane1: Equine Liver lysate; Lane2: Cavia Liver lysate; Lane3: Canine Liver lysate Primary Ab: 0.2μg/ml Rabbit Anti-Mouse WNT5A Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Western Blot; Sample: Mouse Liver lysate;
Primary Ab: 5μg/ml Rabbit Anti-Mouse WNT5A Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human PC3 cell lysate;
Primary Ab: 5μg/ml Rabbit Anti-Mouse WNT5A Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對WNT5A的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別WNT5A。
用法
Western blotting: 0.2-5μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APP549Mu01 | 無翅型MMTV整合位點家族成員5A(WNT5A)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPP549Mu01 | 無翅型MMTV整合位點家族成員5A(WNT5A)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPP549Mu02 | 無翅型MMTV整合位點家族成員5A(WNT5A)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAP549Mu02 | 無翅型MMTV整合位點家族成員5A(WNT5A)多克隆抗體 | WB; IHC; ICC; IP. |
| PAP549Mu01 | 無翅型MMTV整合位點家族成員5A(WNT5A)多克隆抗體 | WB; IHC; ICC; IP. |
| LAP549Mu71 | 無翅型MMTV整合位點家族成員5A(WNT5A)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| LAP549Mu81 | 無翅型MMTV整合位點家族成員5A(WNT5A)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| MAP549Mu21 | 無翅型MMTV整合位點家族成員5A(WNT5A)單克隆抗體 | WB |
| SEP549Mu | 無翅型MMTV整合位點家族成員5A(WNT5A)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMP549Mu | 無翅型MMTV整合位點家族成員5A(WNT5A)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSP549Mu01 | 無翅型MMTV整合位點家族成員5A(WNT5A)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Liver International | Secreted frizzled-related protein 5 (Sfrp5) decreases hepatic stellate cell activation and liver fibrosis [Pubmed:25488180] |
| Journal of Physiology and Biochemistry | Wnt5a reverses the inhibitory effect of hyperoxia on transdifferentiation of alveolar epithelial type II cells to type I cells [PubMed: 26547443] |
| Liver | Secreted frizzled‐related protein 5 (Sfrp5) decreases hepatic stellate cell activation and liver fibrosis [PubMed: 25488180] |
| Transl Res | Wnt5a is expressed in spondyloarthritis and exerts opposite effects on enthesis and bone in murine organ and cell cultures [PubMed: 26163991] |
| 不確定雜志名 | Untersuchungen der Serum-Konzentrationen des proinflammatorischen Glykoproteins wnt-5a und seines antiinflammatorischen Inhibitors sFRP-5 bei an … [servlets:MCRFileNodeServlet] |
| Life Sciences | Hepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways. [pubmed:28754618] |
| Arteriosclerosis, Thrombosis, and?Vascular Biology? | Effect of SFRP5 (Secreted Frizzled–Related Protein 5) on the WNT5A (Wingless-Type Family Member 5A)-Induced Endothelial Dysfunction and Its Relevance With?… [Pubmed:29674475] |
| Stem?Cells | Reduced Cell Division Control Protein 42 Activity Compromises Hematopoiesis‐Supportive Function of Fanconi Anemia Mesenchymal Stromal Cells [Pubmed:29377497] |
| Science Translational Medicine | Adipose tissue–derived WNT5A regulates vascular redox signaling in obesity via USP17/RAC1-mediated activation of NADPH oxidases [Pubmed: 31534019] |
| J Clin Endocrinol Metab | Role of wnt5a in Metabolic Inflammation in Humans [Pubmed: 30137542] |
| Cancer Research | Host Wnt5a Potentiates Microenvironmental Regulation of Ovarian Cancer Metastasis [Pubmed: 31932454] |
| CLINICAL BIOCHEMISTRY | Sfrp5/Wnt5a and Leptin/adiponectin levels in the serum and the periarterial adipose tissue of patients with peripheral arterial occlusive disease [Pubmed: 33188773] |
| Sao Paulo Medical Journal | Association of serum levels of secreted frizzled-related protein 5 and Wnt member 5a with glomerular filtration rate in patients with type 2 diabetes mellitus and … [Pubmed: 32491086] |
| Biomaterials | An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties [34823885] |
| PLoS One | Association of circulating MR-proADM with all-cause and cardiovascular mortality in the general population: Results from the KORA F4 cohort study [Pubmed:34990470] |
| J Clin Periodontol | Association between bone turnover markers and periodontitis: A population‐based cross‐sectional study [Pubmed:35569034] |
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