無翅型MMTV整合位點(diǎn)家族成員5A(WNT5A)多克隆抗體(生物素標(biāo)記)
Biotin-Linked Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)
- 編號(hào)LAP549Mu71
- 物種Mus musculus (Mouse,小鼠) 相同的名稱,不同的物種。
- 來源抗體標(biāo)記
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物生物素
- 原抗體 -
- 緩沖液成份磷酸鹽緩沖液(pH7.4,含有0.02% NaN3和50%甘油)
- 性狀液體
- 濃度500μg/mL
- 且適物種Homo sapiens (Human,人), Rattus norvegicus (Rat,大鼠)
- 應(yīng)用WB; IHC; ICC.
如果抗體需用于流式細(xì)胞術(shù),請(qǐng)參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 785 ¥ 1831 ¥ 2616 ¥ 6540 ¥ 26160
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特異性
該抗體是針對(duì)WNT5A的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別WNT5A。
用法
免疫印跡:0.2-2μg/mL;1:250-2500
免疫組織化學(xué):5-20μg/mL;1:25-100
免疫細(xì)胞化學(xué):5-20μg/mL;1:25-100
最佳稀釋倍數(shù)最終由用戶決定。
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。
贈(zèng)品
增值服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
編號(hào) | 適用物種:Mus musculus (Mouse,小鼠) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
APP549Mu01 | 無翅型MMTV整合位點(diǎn)家族成員5A(WNT5A)活性蛋白 | Cell?culture;?Activity?Assays. |
RPP549Mu01 | 無翅型MMTV整合位點(diǎn)家族成員5A(WNT5A)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPP549Mu02 | 無翅型MMTV整合位點(diǎn)家族成員5A(WNT5A)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAP549Mu02 | 無翅型MMTV整合位點(diǎn)家族成員5A(WNT5A)多克隆抗體 | WB; IHC; ICC; IP. |
PAP549Mu01 | 無翅型MMTV整合位點(diǎn)家族成員5A(WNT5A)多克隆抗體 | WB; IHC; ICC; IP. |
LAP549Mu71 | 無翅型MMTV整合位點(diǎn)家族成員5A(WNT5A)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
LAP549Mu81 | 無翅型MMTV整合位點(diǎn)家族成員5A(WNT5A)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
MAP549Mu21 | 無翅型MMTV整合位點(diǎn)家族成員5A(WNT5A)單克隆抗體 | WB |
SEP549Mu | 無翅型MMTV整合位點(diǎn)家族成員5A(WNT5A)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMP549Mu | 無翅型MMTV整合位點(diǎn)家族成員5A(WNT5A)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSP549Mu01 | 無翅型MMTV整合位點(diǎn)家族成員5A(WNT5A)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Liver International | Secreted frizzled-related protein 5 (Sfrp5) decreases hepatic stellate cell activation and liver fibrosis [Pubmed:25488180] |
Journal of Physiology and Biochemistry | Wnt5a reverses the inhibitory effect of hyperoxia on transdifferentiation of alveolar epithelial type II cells to type I cells [PubMed: 26547443] |
Liver | Secreted frizzled‐related protein 5 (Sfrp5) decreases hepatic stellate cell activation and liver fibrosis [PubMed: 25488180] |
Transl Res | Wnt5a is expressed in spondyloarthritis and exerts opposite effects on enthesis and bone in murine organ and cell cultures [PubMed: 26163991] |
不確定雜志名 | Untersuchungen der Serum-Konzentrationen des proinflammatorischen Glykoproteins wnt-5a und seines antiinflammatorischen Inhibitors sFRP-5 bei an … [servlets:MCRFileNodeServlet] |
Life Sciences | Hepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways. [pubmed:28754618] |
Arteriosclerosis, Thrombosis, and?Vascular Biology? | Effect of SFRP5 (Secreted Frizzled–Related Protein 5) on the WNT5A (Wingless-Type Family Member 5A)-Induced Endothelial Dysfunction and Its Relevance With?… [Pubmed:29674475] |
Stem?Cells | Reduced Cell Division Control Protein 42 Activity Compromises Hematopoiesis‐Supportive Function of Fanconi Anemia Mesenchymal Stromal Cells [Pubmed:29377497] |
Science Translational Medicine | Adipose tissue–derived WNT5A regulates vascular redox signaling in obesity via USP17/RAC1-mediated activation of NADPH oxidases [Pubmed: 31534019] |
J Clin Endocrinol Metab | Role of wnt5a in Metabolic Inflammation in Humans [Pubmed: 30137542] |
Cancer Research | Host Wnt5a Potentiates Microenvironmental Regulation of Ovarian Cancer Metastasis [Pubmed: 31932454] |
CLINICAL BIOCHEMISTRY | Sfrp5/Wnt5a and Leptin/adiponectin levels in the serum and the periarterial adipose tissue of patients with peripheral arterial occlusive disease [Pubmed: 33188773] |
Sao Paulo Medical Journal | Association of serum levels of secreted frizzled-related protein 5 and Wnt member 5a with glomerular filtration rate in patients with type 2 diabetes mellitus and … [Pubmed: 32491086] |
Biomaterials | An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties [34823885] |
PLoS One | Association of circulating MR-proADM with all-cause and cardiovascular mortality in the general population: Results from the KORA F4 cohort study [Pubmed:34990470] |
J Clin Periodontol | Association between bone turnover markers and periodontitis: A population‐based cross‐sectional study [Pubmed:35569034] |