載脂蛋白A1(APOA1)重組蛋白
Recombinant Apolipoprotein A1 (APOA1)
Apo-A1; ApoA-1 Milano; ProapoA-I; Proapolipoprotein A-I; Truncated apolipoprotein A-I
- 編號RPA519Ra01
- 物種Rattus norvegicus (Rat,大鼠) 相同的名稱,不同的物種。
- 來源原核表達
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測定)
- 亞細胞定位分泌
- 預測分子量31.1kDa
- 實際分子量27kDa(差異分析請參閱說明書)
- 片段與標簽Asp25~Ala259 with N-terminal His Tag
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點5.5
-
應用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,請參見活性蛋白。 - 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 1046 ¥ 2616 ¥ 5232 ¥ 15696 ¥ 39240
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序列
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月,-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當?shù)臈l件下存儲,損失率低于5%。
增值服務
相關產(chǎn)品
編號 | 適用物種:Rattus norvegicus (Rat,大鼠) | 應用(僅供研究使用,不用于臨床診斷!) |
RPA519Ra01 | 載脂蛋白A1(APOA1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA519Ra01 | 載脂蛋白A1(APOA1)多克隆抗體 | WB; IHC; ICC; IP. |
LAA519Ra71 | 載脂蛋白A1(APOA1)多克隆抗體(生物素標記) | WB; IHC; ICC. |
MAA519Ra22 | 載脂蛋白A1(APOA1)單克隆抗體 | WB; IHC; ICC; IP. |
MAA519Ra21 | 載脂蛋白A1(APOA1)單克隆抗體 | WB; IHC; ICC; IP. |
SEA519Ra | 載脂蛋白A1(APOA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA519Ra | 載脂蛋白A1(APOA1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSA519Ra01 | 載脂蛋白A1(APOA1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
European Journal of Applied Physiology | Endurance training enhances ABCA1 expression in rat small intestine [PubMed: 19629515] |
Official Journal of the INWR | Effect of eight weeks of wrestling and circuit fitness training on Apo lipoportein A-I and lymphocyte ABCA1 gene expression in well-trained wrestler. [Wrestling: source] |
Biochemical and Biophysical Research Communications | Treadmill exercise enhances ABCA1 expression in rat liver [ScienceDirect: S0006291X0701488X] |
World Journal of Sport Sciences | The Effect of Eight Weeks of Wrestling and Wrestling Technique Based Circuit Training on Lymphocyte ABCA1 Gene Expression and Plasma Apolipoprotein A-1 [Idosi: source] |
Biochimica et Biophysica Acta | Hepatic lipase- and endothelial lipase-deficiency in mice promotes macrophage-to-feces RCT and HDL antioxidant properties [PubMed: 23328279 ] |
Biomarkers | The association of a distinct plasma proteomic profile with the cervical high-grade squamous intraepithelial lesion of Uyghur women: a 2D liquid-phase chromatography/mass spectrometry study [PubMed: 22458349] |
Journal of the American Heart Association | MicroRNA-33 Deficiency Reduces the Progression of Atherosclerotic Plaque in ApoE?/? Mice [PubMed: PMC3540673] |
PLoS ONE | Apolipoprotein AI Is a Potential Mediator of Remote Ischemic Preconditioning [Plosone: Source] |
Molecular Genetics and Metabolism | DYRK1A overexpression decreases plasma lecithin: cholesterol acyltransferase activity and apolipoprotein AI levels [Pubmed: 23920041] |
Endocrinology | Deficiency of Clusterin Exacerbates High-Fat Diet-Induced Insulin Resistance in Male Mice [Pubmed: 24684302] |
Molecular Genetics and Metabolism Reports | Corrective effects of hepatotoxicity by hepatic Dyrk1a gene delivery in mice with intermediate hyperhomocysteinemia [Sciencedirect:S2214426915000026] |
J Psychopharmacol | Anti-atherogenic properties of high-density lipoproteins in psychiatric patients before and after two months of atypical anti-psychotic therapy [PubMed: 26253619] |
Biochim Biophys Acta | Culturing of HepG2 cells with human serum improve their functionality and suitability in studies of lipid metabolism [PubMed: 26515253] |
Pharmacol Res Perspect | Efficacy of tomato concentrates in mouse models of dyslipidemia and cancer [PubMed: 26171234] |
Clin Exp Nephrol | Differentially expressed urinary biomarkers in children with idiopathic nephrotic syndrome [PubMed: 26351173] |
Oncogene | Apolipoprotein A-I inhibits experimental colitis and colitis-propelled carcinogenesis [PubMed: 26279300] |
Oncogene | Apolipoprotein AI inhibits experimental colitis and colitis-propelled carcinogenesis [Pubmed:26279300] |
Allergy | Serum levels of 9α,11β-PGF2 and apolipoprotein A1 achieve high predictive power as biomarkers of anaphylaxis. [pubmed:28378321] |
Journal of Chromatography A | Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858] |
11 | High-Density Lipoproteins Exert Pro-inflammatory Effects on Macrophages via Passive Cholesterol Depletion and PKC-NF-kB/STAT1-IRF1 Signaling [pubmed:27866837] |
Biomedical Science Letters | Effect of Ginkgo biloba Extract (EGb 761) on Serum Cholesterol Levels in Wild-type C57Bl/6 Mice [10.15616/BSL.2017.23.2.80] |
ACS?Biomaterials Science?&?Engineering | Enhanced Antiatherosclerotic Efficacy of Statin-Loaded Reconstituted High-Density Lipoprotein via Ganglioside GM1 Modification [abs:10.1021] |
Food?Research International | Wild Lonicera caerulea berry polyphenol extract reduces cholesterol accumulation and enhances antioxidant capacity in vitro and in vivo [Pubmed:29580541] |
Journal of The Science of Food and Agriculture | Consumption of orange fermented beverage improves antioxidant status and reduces peroxidation lipid and inflammatory markers in healthy humans [Pubmed:29124773] |
Toxicon | A membrane disrupting toxin from wasp venom underlies the molecular mechanism of tissue damage [Pubmed:29654869] |
European?Journal of Pharmacology | The effect of exenatide (a GLP-1 analog) and sitagliptin (a DPP-4 inhibitor) on plasma platelet-activating factor acetylhydrolase (PAF-AH) activity and concentration in?… [Pubmed: 30768981] |
European Journal of Preventive Cardiology | High-intensity interval training has a greater effect on reverse cholesterol transport elements compared with moderate-intensity continuous training in obese male rats [Pubmed: 31718266] |
Diagnostics | Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J [Pubmed: 32585848] |
VETERINARY PATHOLOGY | Pathology and Proteomics-Based Diagnosis of Localized Light-Chain Amyloidosis in Dogs and Cats [Pubmed: 32880234] |
Nat Commun | GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity [34853313] |
Cardiovasc Toxicol | Interplay of Obesity, Ethanol, and Contaminant Mixture on Clinical Profiles of Cardiovascular and Metabolic Diseases: Evidence from an Animal Study [Pubmed:35429258] |
Peptides | Neuropeptide Y promotes hepatic apolipoprotein A1 synthesis and secretion through neuropeptide Y Y5 receptor [Pubmed:35660638] |
Journal of Functional Foods | Castanea mollissima shell polyphenols regulate JAK2 and PPARγ expression to suppress inflammation and lipid accumulation by inhibiting M1 macrophages?… [] |
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