堿性磷酸酶(ALP)檢測試劑盒(化學(xué)發(fā)光免疫分析法)
CLIA Kit for Alkaline Phosphatase (ALP)
AKP; ALKP; Basic Phosphatase
- 編號SCB472Bo
- 物種Bos taurus; Bovine (Cattle，牛) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長2h, 40min
- 檢測范圍68.6-50000pg/mL
- 靈敏度最小可檢測劑量小于等于25.3pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 4234 ¥ 6048 ¥ 27216 ¥ 51408 ¥ 423360
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測堿性磷酸酶(ALP)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的堿性磷酸酶(ALP)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 82-91 | 86 |
| EDTA plasma(n=5) | 80-96 | 82 |
| heparin plasma(n=5) | 83-90 | 87 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的堿性磷酸酶(ALP)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中堿性磷酸酶(ALP)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-103% | 85-96% | 82-91% | 78-101% |
| EDTA plasma(n=5) | 94-103% | 85-103% | 85-101% | 96-105% |
| heparin plasma(n=5) | 78-99% | 90-97% | 87-101% | 78-95% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100μL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加底物100µL，37°C孵育10分鐘；
8. 讀數(shù)。
實驗原理
將堿性磷酸酶(ALP)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的堿性磷酸酶(ALP)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的堿性磷酸酶(ALP)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入底物。加入底物后會產(chǎn)生輝光型光信號。發(fā)射光強度和樣品中的堿性磷酸酶(ALP)呈正相關(guān)。用化學(xué)發(fā)光儀測定相對光單位（RLU），計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Bos taurus; Bovine (Cattle，牛) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB472Bo01 | 堿性磷酸酶(ALP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB472Bo01 | 堿性磷酸酶(ALP)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB472Bo71 | 堿性磷酸酶(ALP)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| SEB472Bo | 堿性磷酸酶(ALP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCB472Bo | 堿性磷酸酶(ALP)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB472Bo | 堿性磷酸酶(ALP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB472Bo01 | 堿性磷酸酶(ALP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Clinical Oral Implants Research | Comparison of platelet rich fibrin and collagen as osteoblast-seeded scaffolds for bone tissue engineering applications [Wiley: source] |
| Die Pharmazie - An International Journal of Pharmaceutical Sciences | The role of atorvastatin in bone metabolism in male albino Wistar rats [Ingenta: art00010] |
| 22 | Combined effect of strontium and zoledronate on hydroxyapatite structure and bone cell responses [ScienceDirect: S0142961214003135] |
| Biomed Mater.? | Directing chondrogenic differentiation of mesenchymal stem cells with a solid-supported chitosan thermogel for cartilage tissue engineering [Pubmed:24770944] |
| Journal of Colloid and Interface Science | Strontium and zoledronate hydroxyapatites graded composite coatings for bone prostheses [Pubmed:25706198] |
| Bone | The active role of osteoporosis in the interaction between osteoblasts and bone metastases [PubMed: 26057367] |
| Macromolecular Bioscience | Multi‐Layered Scaffolds for Osteochondral Tissue Engineering: In Vitro Response of Co‐Cultured Human Mesenchymal Stem Cells [PubMed: 26126665] |
| journal of hard Tissue Biology | Biological Evaluation of a Prototype Material made of Polyglycolic Acid and Hydroxyapatite [Article: Jhtb] |
| Colloids Surf B Biointerfaces | Antiresorption implant coatings based on calcium alendronate and octacalcium phosphate deposited by matrix assisted pulsed laser evaporation [PubMed: 26445021] |
| Gels | On the Mechanism of Drug Release from Polysaccharide Hydrogels Cross-Linked with Magnetite Nanoparticles by Applying Alternating Magnetic Fields: the Case of DOXO Delivery [2310-2861: 1] |
| Acta Biomater | Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: an in vitro osteoblast-osteoclast-endothelial cell co-culture study [PubMed: 26689470] |
| Sci Rep | A composite scaffold of MSC affinity peptide-modified demineralized bone matrix particles and chitosan hydrogel for cartilage regeneration [PubMed: 26632447] |
| Acta Biomaterialia | Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: An in vitro osteoblast–osteoclast–endothelial cell co-culture study [Pubmed:26689470] |
| PLoS One. | Extracorporeal shock waves alone or combined with raloxifene promote bone formation and suppress resorption in ovariectomized rats. [pubmed:28158228] |
| Scientific Reports | Adiponectin?protects?the?rats?liver?against?chronic?intermittent?hypoxia?induced?injury?throughAMP-activated?protein?kinase?pathway. [pubmed:27678302] |
| J Cell Physiol. | An advanced tri-culture model to evaluate the dynamic interplay among osteoblasts, osteoclasts, and endothelial cells. [pubmed:28240358] |
| Biomedicine & Pharmacotherapy | Anti-osteoporotic effects of an antidepressant tianeptine on ovariectomized rats [S0753332216322752] |
| Journal of Inorganic Biochemistry | Biomimetic fabrication of antibacterial calcium phosphates mediated by polydopamine. [pubmed:29049953] |
| Biomedicine & pharmacotherapy | Icaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation. [pubmed:28742995] |
| Journal of?Clinical?Periodontology? | Peroxisome proliferator‐activated receptor γ plays dual roles on experimental periodontitis in rats [Pubmed:29574908] |
| Macromolecular?Bioscience | Strontium‐Substituted Hydroxyapatite‐Gelatin Biomimetic Scaffolds Modulate Bone Cell Response [Pubmed:29877029] |
| Journal of Inorganic Biochemistry | Gradient coatings of strontium hydroxyapatite/zinc β-tricalcium phosphate as a tool to modulate osteoblast/osteoclast response [Pubmed:29525694] |
| DNA?and?Cell?Biology | miRNA-133a-5p Inhibits the Expression of Osteoblast Differentiation-Associated Markers by Targeting the 3′ UTR of [Pubmed:29359964] |
| Bone | The sole and combined effect of simvastatin and platelet rich fibrin as a filling material in induced bone defect in tibia of albino rats [Pubmed: 30208342] |
| Experimental Gerontology | The decline of whole-body glucose metabolism in ovariectomized rats [Pubmed: 30292771] |
| BMC?Complementary and Alternative?Medicine? | Hepatoprotective activity of Erythrina× neillii leaf extract and characterization of its phytoconstituents [Pubmed: 28095910] |
| Journal of Hematology &?Oncology | Multiple myeloma-derived exosomes are enriched of amphiregulin (AREG) and activate the epidermal growth factor pathway in the bone microenvironment?… [Pubmed: 30621731] |
| JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS | CoCr porous scaffolds manufactured via selective laser melting in orthopedics: Topographical, mechanical, and biological characterization [Pubmed: 30689288] |
| Chemico-Biological?Interactions | Lead exposure inhibits osteoblastic differentiation and inactivates the canonical Wnt signal and recovery by icaritin in MC3T3-E1 subclone 14 cells [Pubmed: 30731080] |
| Journal of Orthopaedic Surgery and Research | Macrophage inhibits the osteogenesis of fibroblasts in ultrahigh molecular weight polyethylene (UHMWPE) wear particle-induced osteolysis [] |
| Bangladesh Journal of Medical Science | Zn as the Factor of Alkaline Phosphatase in Periodontal Patients consuming Minangkabau food in West Sumatra, Indonesia [] |
| Molecular Therapy-Nucleic Acids | AAV-anti-miR-214 prevents collapse of the femoral head in osteonecrosis by regulating osteoblast and osteoclast activities [Pubmed: 31739209] |
| Journal of Korean Neurosurgical Society | Rutin Improves Bone Histomorphometric Values by Reduction of Osteoclastic Activity in Osteoporosis Mouse Model Induced by Bilateral Ovariectomy [Pubmed: 32172552] |
| Journal of Applied Biomaterials & Functional Materials | Effects of tricalcium silicate/sodium alginate/calcium sulfate hemihydrate composite cements on osteogenic performances in vitro and in vivo [Pubmed: 32138579] |
| Nutrients | Osteoprotective Activity and Metabolite Fingerprint via UPLC/MS and GC/MS of Lepidium sativum in Ovariectomized Rats [Pubmed: 32668691] |
| International Journal of Clinical and Experimental Pathology | Matrilin-2 is a novel prognostic marker in osteosarcoma [] |
| Revista Argentina de Clínica Psicológica | Research on the Mechanism of miR-142-5p's Promotion of the Function and Mineralization of Osteoblasts by Down-regulating the Expression of WWP1 Gene [] |
| МАРКЕРЫ КОСТНОГО РЕМОДЕЛИРОВАНИЯ ПРИ ЗАМЕЩЕНИИ ДЕФЕКТАТРАБЕКУЛЯРНОЙ КОСТНОЙ ТКАНИ РЕЗОРБИРУЕМЫМИ И?… [] | |
| Ceramics International | Platinum nanoparticles supported on functionalized hydroxyapatite: Anti-oxidant properties and bone cells response [] |
| Bioelectromagnetics | Static Magnetic Field (2¨C4 T) Improves Bone Microstructure and Mechanical Properties by Coordinating Osteoblast/Osteoclast Differentiation in Mice [33655538] |
| PeerJ | From hair to liver: emerging application of hair follicle mesenchymal stem cell transplantation reverses liver cirrhosis by blocking the TGF-β/Smad signaling?… [Pubmed:35186473] |
| Cell Death Discov | ECM1 modified HF-MSCs targeting HSC attenuate liver cirrhosis by inhibiting the TGF-β/Smad signaling pathway [Pubmed:35136027] |
| Int J Mol Sci | Antiosteoporotic Nanohydroxyapatite Zoledronate Scaffold Seeded with Bone Marrow Mesenchymal Stromal Cells for Bone Regeneration: A 3D In Vitro Model [Pubmed:35682677] |
| Journal of Bionic Engineering | Effect of Antibacterial Enoxacin on the Properties of Injectable Nano-hydroxyapatite/Polyurethane Cement for Bone Repairing [] |
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