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載脂蛋白E(APOE)檢測試劑盒(化學(xué)發(fā)光免疫分析法)

CLIA Kit for Apolipoprotein E (APOE)

Apo-E; AD2; Apoprotein; Alzheimer Disease 2(E4-Associated,Late Onset

  • 載脂蛋白E(APOE)檢測試劑盒(化學(xué)發(fā)光免疫分析法) 產(chǎn)品包裝(模擬)
  • 載脂蛋白E(APOE)檢測試劑盒(化學(xué)發(fā)光免疫分析法) 產(chǎn)品包裝(模擬)
  • 載脂蛋白E(APOE)檢測試劑盒(化學(xué)發(fā)光免疫分析法) 實驗結(jié)果圖
  • SCA704Hu.jpg 標(biāo)準曲線圖
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒用于檢測載脂蛋白E(APOE),經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制,不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測,因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的載脂蛋白E(APOE)(加標(biāo)樣品),重復(fù)測定并計算其均值,回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 97-105 101
EDTA plasma(n=5) 98-105 102
heparin plasma(n=5) 85-103 90

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復(fù)測定8次,分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的載脂蛋白E(APOE),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中載脂蛋白E(APOE)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 88-101% 91-99% 99-105% 84-102%
EDTA plasma(n=5) 86-99% 87-104% 96-104% 87-97%
heparin plasma(n=5) 85-103% 91-101% 82-101% 91-98%

穩(wěn)定性

經(jīng)測定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標(biāo)準品、試劑及樣本的準備;
2. 加樣(標(biāo)準品及樣本)100µL,37°C孵育1小時;
3. 吸棄,加檢測溶液A100μL,37°C孵育1小時;
4. 洗板3次;
5. 加檢測溶液B100µL,37°C孵育30分鐘;
6. 洗板5次;
7. 加底物100µL,37°C孵育10分鐘;
8. 讀數(shù)。

實驗原理

將載脂蛋白E(APOE)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標(biāo)準品或標(biāo)本,其中的載脂蛋白E(APOE)與連接于固相載體上的抗體結(jié)合,然后加入生物素化的載脂蛋白E(APOE)抗體,將未結(jié)合的生物素化抗體洗凈后,加入HRP標(biāo)記的親和素,再次徹底洗滌后加入底物。加入底物后會產(chǎn)生輝光型光信號。發(fā)射光強度和樣品中的載脂蛋白E(APOE)呈正相關(guān)。用化學(xué)發(fā)光儀測定相對光單位(RLU),計算樣品濃度。

相關(guān)產(chǎn)品

編號 適用物種:Homo sapiens (Human,人) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
EPA704Hu61 載脂蛋白E(APOE)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA704Hu61 載脂蛋白E(APOE)活性蛋白 Cell?culture;?Activity?Assays.
RPA704Hu01 載脂蛋白E(APOE)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA704Hu01 載脂蛋白E(APOE)多克隆抗體 WB; IHC; ICC; IP.
MAA704Hu21 載脂蛋白E(APOE)單克隆抗體 WB; IHC; ICC; IP.
MAA704Hu22 載脂蛋白E(APOE)單克隆抗體 WB; IHC; ICC; IP.
SEA704Hu 載脂蛋白E(APOE)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA704Hu 載脂蛋白E(APOE)檢測試劑盒(化學(xué)發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA704Hu 載脂蛋白E(APOE)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA704Hu01 載脂蛋白E(APOE)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志 參考文獻
Seizure - European Journal of Epilepsy Cerebrospinal fluid apolipoprotein E concentration decreases after seizure [Seizure: PIIS1059131109002441]
Biochimica et Biophysica Acta Hepatic lipase- and endothelial lipase-deficiency in mice promotes macrophage-to-feces RCT and HDL antioxidant properties [PubMed: 23328279 ]
Medical Oncology Expression and clinical significance of apolipoprotein E in pancreatic ductal adenocarcinoma [Pubmed: 23609192]
Diabetes. Adipocyte Atp-Binding Cassette G1 Promotes Triglyceride Storage, Fat Mass Growth And Human Obesity [Pubmed:25249572]
renal failure Frequencies of apolipoprotein E alleles in depressed patients undergoing hemodialysis – a case-control study [PubMed: 25707516]
Biochim Biophys Acta Culturing of HepG2 cells with human serum improve their functionality and suitability in studies of lipid metabolism [PubMed: 26515253]
Diabetes Adipocyte ATP-binding cassette G1 promotes triglyceride storage, fat mass growth, and human obesity [PubMed: 25249572]
Scientific Reports Adaptive?immunity?against?gut?microbiota?enhances?apoE-mediated?immune?regulation?andreduces?atherosclerosis?and?western-diet-related?inflammation. [pubmed:27383250]
Biochemical Pharmacology? Differential effects of apoE and apoJ mimetic peptides on the action of an anti-Aβ scFv in 3xTg-AD mice [Pubmed:30026023]
Oncotarget Deletion of plasma Phospholipid Transfer Protein (PLTP) increases microglial phagocytosis and reduces cerebral amyloid-β deposition in the J20 mouse?… [Pubmed:29731975]
Proteomics Clinical Applications Comparative proteomics analysis of serum proteins in gestational diabetes during early and middle stages of pregnancy [Pubmed: 31162828]
Nature Communications Hepatic HuR modulates lipid homeostasis in response to high-fat diet [Pubmed: 32546794]
VETERINARY PATHOLOGY Pathology and Proteomics-Based Diagnosis of Localized Light-Chain Amyloidosis in Dogs and Cats [Pubmed: 32880234]
Journal of Clinical Medicine The Significance of Apolipoprotein E Measurement in the Screening of Fetal Down Syndrome [33321701]
Cancer Cell Int Discovery and validation of FBLN1 and ANT3 as potential biomarkers for early detection of cervical cancer [33602229]
Nat Commun GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity [34853313]
Talanta Iridium nanoclusters as high sensitive-tunable elemental labels for immunoassays: Determination of IgE and APOE in aqueous humor by inductively coupled?… [Pubmed:35378356]
Analytica Chimica Acta Gold nanoclusters as elemental label for the sequential quantification of apolipoprotein E and metallothionein 2A in individual human cells of the retinal?… [Pubmed:35361429]
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