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基質(zhì)金屬蛋白酶2(MMP2)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)

CLIA Kit for Matrix Metalloproteinase 2 (MMP2)

CLG4; CLG4A; MMP-II; MONA; TBE-1; PEX; Gelatinase A; 72kDa Gelatinase; 72kDa Type IV Collagenase

  • 基質(zhì)金屬蛋白酶2(MMP2)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) 產(chǎn)品包裝(模擬)
  • 基質(zhì)金屬蛋白酶2(MMP2)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) 產(chǎn)品包裝(模擬)
  • 基質(zhì)金屬蛋白酶2(MMP2)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) 實(shí)驗(yàn)結(jié)果圖
  • SCA100Hu.jpg 標(biāo)準(zhǔn)曲線(xiàn)圖
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒用于檢測(cè)基質(zhì)金屬蛋白酶2(MMP2),經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制,不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè),因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的基質(zhì)金屬蛋白酶2(MMP2)(加標(biāo)樣品),重復(fù)測(cè)定并計(jì)算其均值,回收率為測(cè)定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 94-101 98
heparin plasma(n=5) 82-95 87

精密度

精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次,分別計(jì)算不同濃度樣本的平均值及SD值。
批間差:選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定,每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次,分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%

線(xiàn)性

在定值血清及血漿樣本內(nèi)加入適量的基質(zhì)金屬蛋白酶2(MMP2),并倍比稀釋成1:2,1:4,1:8,1:16的待測(cè)樣本,線(xiàn)性范圍即為稀釋后樣本中基質(zhì)金屬蛋白酶2(MMP2)含量的測(cè)定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 79-101% 86-99% 94-103% 82-91%
heparin plasma(n=5) 80-89% 95-102% 90-102% 84-103%

穩(wěn)定性

經(jīng)測(cè)定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響,實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致,尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。

實(shí)驗(yàn)流程

1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備;
2. 加樣(標(biāo)準(zhǔn)品及樣本)100µL,37°C孵育1小時(shí);
3. 吸棄,加檢測(cè)溶液A100μL,37°C孵育1小時(shí);
4. 洗板3次;
5. 加檢測(cè)溶液B100µL,37°C孵育30分鐘;
6. 洗板5次;
7. 加底物100µL,37°C孵育10分鐘;
8. 讀數(shù)。

實(shí)驗(yàn)原理

將基質(zhì)金屬蛋白酶2(MMP2)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本,其中的基質(zhì)金屬蛋白酶2(MMP2)與連接于固相載體上的抗體結(jié)合,然后加入生物素化的基質(zhì)金屬蛋白酶2(MMP2)抗體,將未結(jié)合的生物素化抗體洗凈后,加入HRP標(biāo)記的親和素,再次徹底洗滌后加入底物。加入底物后會(huì)產(chǎn)生輝光型光信號(hào)。發(fā)射光強(qiáng)度和樣品中的基質(zhì)金屬蛋白酶2(MMP2)呈正相關(guān)。用化學(xué)發(fā)光儀測(cè)定相對(duì)光單位(RLU),計(jì)算樣品濃度。

相關(guān)產(chǎn)品

編號(hào) 適用物種:Homo sapiens (Human,人) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
EPA100Hu62 基質(zhì)金屬蛋白酶2(MMP2)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA100Hu62 基質(zhì)金屬蛋白酶2(MMP2)活性蛋白 Cell?culture;?Activity?Assays.
RPA100Hu01 基質(zhì)金屬蛋白酶2(MMP2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA100Hu01 基質(zhì)金屬蛋白酶2(MMP2)活性蛋白 Cell?culture;?Activity?Assays.
APA100Hu61 基質(zhì)金屬蛋白酶2(MMP2)活性蛋白 Cell?culture;?Activity?Assays.
RPA100Hu03 基質(zhì)金屬蛋白酶2(MMP2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPA100Hu63 基質(zhì)金屬蛋白酶2(MMP2)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPA100Hu61 基質(zhì)金屬蛋白酶2(MMP2)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA100Hu02 基質(zhì)金屬蛋白酶2(MMP2)活性蛋白 Cell?culture;?Activity?Assays.
RPA100Hu02 基質(zhì)金屬蛋白酶2(MMP2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA100Hu02 基質(zhì)金屬蛋白酶2(MMP2)多克隆抗體 WB; IHC; ICC; IP.
PAA100Hu01 基質(zhì)金屬蛋白酶2(MMP2)多克隆抗體 WB; IHC; ICC; IP.
LAA100Hu71 基質(zhì)金屬蛋白酶2(MMP2)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
LAA100Hu81 基質(zhì)金屬蛋白酶2(MMP2)多克隆抗體(異硫氰酸熒光素標(biāo)記) WB; IHC; ICC; IF.
FAA100Hu41 抗基質(zhì)金屬蛋白酶2(MMP2)多克隆抗體(藻紅素標(biāo)記) Flow cytometry.
MAA100Hu27 基質(zhì)金屬蛋白酶2(MMP2)單克隆抗體 WB; IHC; ICC; IP.
MAA100Hu23 基質(zhì)金屬蛋白酶2(MMP2)單克隆抗體 WB; IHC; ICC; IP.
MAA100Hu26 基質(zhì)金屬蛋白酶2(MMP2)單克隆抗體 WB; IHC; ICC; IP.
MAA100Hu22 基質(zhì)金屬蛋白酶2(MMP2)單克隆抗體 WB; IHC
MAA100Hu21 基質(zhì)金屬蛋白酶2(MMP2)單克隆抗體 WB; IHC; ICC; IP.
FAA100Hu02 抗基質(zhì)金屬蛋白酶2(MMP2)單克隆抗體 Flow cytometry.
FAA100Hu04 抗基質(zhì)金屬蛋白酶2(MMP2)單克隆抗體 Flow cytometry.
FAA100Hu06 抗基質(zhì)金屬蛋白酶2(MMP2)單克隆抗體 Flow cytometry.
FAA100Hu08 抗基質(zhì)金屬蛋白酶2(MMP2)單克隆抗體 Flow cytometry.
SEA100Hu 基質(zhì)金屬蛋白酶2(MMP2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA100Hu 基質(zhì)金屬蛋白酶2(MMP2)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA100Hu 基質(zhì)金屬蛋白酶2(MMP2)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

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