轉(zhuǎn)化生長因子β3(TGFb3)檢測試劑盒(化學(xué)發(fā)光免疫分析法)
CLIA Kit for Transforming Growth Factor Beta 3 (TGFb3)
TGF-B3; LAP; Latency-associated peptide
- 編號SCB949Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長2h, 40min
- 檢測范圍1.37-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于0.57pg/mL.
- 樣本類型Serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 3528 ¥ 5040 ¥ 22680 ¥ 42840 ¥ 352800
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測轉(zhuǎn)化生長因子β3(TGFb3)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的轉(zhuǎn)化生長因子β3(TGFb3)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 90-98 | 94 |
| EDTA plasma(n=5) | 92-104 | 101 |
| heparin plasma(n=5) | 87-99 | 94 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的轉(zhuǎn)化生長因子β3(TGFb3)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中轉(zhuǎn)化生長因子β3(TGFb3)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-103% | 81-104% | 80-90% | 80-95% |
| EDTA plasma(n=5) | 81-102% | 86-94% | 81-92% | 78-99% |
| heparin plasma(n=5) | 80-98% | 99-105% | 79-98% | 80-101% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測溶液A100μL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加底物100µL，37°C孵育10分鐘；
8. 讀數(shù)。
實(shí)驗(yàn)原理
將轉(zhuǎn)化生長因子β3(TGFb3)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的轉(zhuǎn)化生長因子β3(TGFb3)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的轉(zhuǎn)化生長因子β3(TGFb3)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入底物。加入底物后會產(chǎn)生輝光型光信號。發(fā)射光強(qiáng)度和樣品中的轉(zhuǎn)化生長因子β3(TGFb3)呈正相關(guān)。用化學(xué)發(fā)光儀測定相對光單位（RLU），計(jì)算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB949Hu02 | 轉(zhuǎn)化生長因子β3(TGFb3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB949Hu02 | 轉(zhuǎn)化生長因子β3(TGFb3)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB949Hu01 | 轉(zhuǎn)化生長因子β3(TGFb3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB949Hu01 | 轉(zhuǎn)化生長因子β3(TGFb3)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAB949Hu01 | 轉(zhuǎn)化生長因子β3(TGFb3)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB949Hu02 | 轉(zhuǎn)化生長因子β3(TGFb3)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB949Hu71 | 轉(zhuǎn)化生長因子β3(TGFb3)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAB949Hu23 | 轉(zhuǎn)化生長因子β3(TGFb3)單克隆抗體 | WB; IHC; ICC; IP. |
| RAB949Hu21 | 轉(zhuǎn)化生長因子β3(TGFb3)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| MAB949Hu22 | 轉(zhuǎn)化生長因子β3(TGFb3)單克隆抗體 | WB |
| MAB949Hu24 | 轉(zhuǎn)化生長因子β3(TGFb3)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB949Hu26 | 轉(zhuǎn)化生長因子β3(TGFb3)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB949Hu25 | 轉(zhuǎn)化生長因子β3(TGFb3)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB949Hu21 | 轉(zhuǎn)化生長因子β3(TGFb3)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB949Hu | 轉(zhuǎn)化生長因子β3(TGFb3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCB949Hu | 轉(zhuǎn)化生長因子β3(TGFb3)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB949Hu | 轉(zhuǎn)化生長因子β3(TGFb3)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| PSB949Hu01 | 轉(zhuǎn)化生長因子β3(TGFb3)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSB949Hu01 | 轉(zhuǎn)化生長因子β3(TGFb3)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source] |
| J Reprod Immunol | The immunomodulating effect of seminal plasma on T cells [PubMed: 25799173] |
| Fertility and Sterility | Influence of vitamin D and transforming growth factor β3 serum concentrations, obesity, and family history on the risk for uterine fibroids [pubmed:27743697] |
| Eur J Pharm Biopharm | New scaffolds encapsulating TGF-β3/BMP-7 combinations driving strong chondrogenic differentiation [pubmed:28087378] |
| Transforme edici büyüme fakt?rü-beta-1 n?tralizan antikoru ve transforme edici büyüme fakt?rü-beta-3'ün trakeal darl?k geli?imi üzerine etkileri [10.5606/tgkdc.dergisi.2017.14302] | |
| Fertility and Sterility | Ulipristal acetate decreases transforming growth factor β3 serum and tumor tissue concentrations in patients with uterine fibroids [Pubmed:29525690] |
| Scientific Reports | TGF-β concentrations and activity are down-regulated in the aqueous humor of patients with neovascular age-related macular degeneration [Pubmed:29795291] |
| Immunology?Letters | The involvement of multifunctional TGF-β and related cytokines in pathogenesis of endometriosis [Pubmed: 30367890] |
| Cell?Reports | Transforming Growth Factor-β3 Regulates Adipocyte Number in Subcutaneous White Adipose Tissue [Pubmed: 30332637] |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Temporal TGF-β Supergene Family Signalling Cues Modulating Tissue Morphogenesis: Chondrogenesis within a Muscle Tissue Model? [Pubmed: 32660137] |
| Equine Vet J | Limited added value of negative pressure wound therapy compared to calcium alginate dressings for second intention healing in a non?\contaminated and??- [34115409] |
| AAPS PharmSciTech | Nanoparticles and Nanostructured Films with TGF-β3: Preparation, Characterization, and Efficacy [34378118] |
