波形蛋白(VIM)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Vimentin (VIM)
- 編號KSB040Hu01
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測抗原
- 可檢測范圍125-8000pg/mL
- 可適靈敏度46pg/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 7182 ¥ 11970 ¥ 21546 ¥ 41895 ¥ 71820
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特異性
試劑盒中的抗體對波形蛋白(VIM)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。
儲存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時(shí)間為1個(gè)月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPB040Hu02 | 波形蛋白(VIM)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APB040Hu01 | 波形蛋白(VIM)活性蛋白 | Cell?culture;?Activity?Assays. |
RPB040Hu01 | 波形蛋白(VIM)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB040Hu02 | 波形蛋白(VIM)多克隆抗體 | WB; IHC; ICC; IP. |
PAB040Hu01 | 波形蛋白(VIM)多克隆抗體 | WB; IHC; ICC/IF |
LAB040Hu71 | 波形蛋白(VIM)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
MAB040Hu24 | 波形蛋白(VIM)單克隆抗體 | WB; IHC |
MAB040Hu25 | 波形蛋白(VIM)單克隆抗體 | WB; IHC; IF |
MAB040Hu21 | 波形蛋白(VIM)單克隆抗體 | WB; IHC; ICC; IP. |
MAB040Hu23 | 波形蛋白(VIM)單克隆抗體 | WB; IHC; ICC; IP. |
MAB040Hu27 | 波形蛋白(VIM)單克隆抗體 | WB; IHC; ICC; IP. |
MAB040Hu22 | 波形蛋白(VIM)單克隆抗體 | WB; IHC; ICC/IF |
FAB040Hu02 | 抗波形蛋白(VIM)單克隆抗體 | Flow cytometry. |
FAB040Hu06 | 抗波形蛋白(VIM)單克隆抗體 | Flow cytometry. |
FAB040Hu04 | 抗波形蛋白(VIM)單克隆抗體 | Flow cytometry. |
FAB040Hu08 | 抗波形蛋白(VIM)單克隆抗體 | Flow cytometry. |
SEB040Hu | 波形蛋白(VIM)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB040Hu | 波形蛋白(VIM)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSB040Hu01 | 波形蛋白(VIM)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Cancer?Epidemiology,?Biomarkers?& Prevention | Aberrant Vimentin Methylation Is Characteristic of Breast Cancer [pubmed:22315367] |
Scientific Reports | Glyoxalase?1-knockdown?in?human?aortic?endothelial?cells - effect on the?proteome?andendothelial?function?estimates. [pubmed:27898103] |
Journal of?Proteomics | HtrA3 is a cellular partner of cytoskeleton proteins and TCP1α chaperonin [Pubmed:29477555] |
Journal of Biological Chemistry | Growth hormone induces Notch1 signaling in podocytes and contributes to proteinuria in diabetic nephropathy [Pubmed: 31511328] |
American Journal of Transplantation | Extracellular vesicles derived from injured vascular tissue promote the formation of tertiary lymphoid structures in vascular allografts [Pubmed: 31729155] |
Lung | Is Vimentin the Cause or Effect of Obstructive Sleep Apnea Development? [Pubmed: 32088750] |
Cell Biol Toxicol | ZNF471 modulates EMT and functions as methylation regulated tumor suppressor with diagnostic and prognostic significance in cervical cancer [33566221] |
CELL BIOLOGY AND TOXICOLOGY | Metastatic suppression by DOC2B is mediated by inhibition of epithelial-mesenchymal transition and induction of senescence [33758996] |
Comparison of Vimentin Levels Between Preeclamptic and Normotensive Pregnant Women [] | |
Advanced glycation end-products associate with podocytopathy in type II diabetic patients [] | |
Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β?… [] |
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