波形蛋白(VIM)單克隆抗體
Monoclonal Antibody to Vimentin (VIM)
- 編號(hào)MAB040Hu27
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來(lái)源單抗制備
- 宿主小鼠
- 效價(jià)(克隆號(hào))C13
- Ig亞型 IgG2a Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無(wú)標(biāo)記物
- 免疫原 RPB040Hu01-波形蛋白(VIM)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度1mg/ml
- 且適物種Rattus norvegicus (Rat，大鼠), Canis familiaris; Canine (Dog，犬), Sus scrofa; Porcine (Pig，豬), Bos taurus; Bovine (Cattle，牛), Chicken (Gallus，雞)
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見(jiàn)流式抗體。 - 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 648 ¥ 1512 ¥ 2160 ¥ 5400 ¥ 21600
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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FITC staining on IF; Samples: Human MCF7 Cells; Primary Ab: 30μg/ml Mouse Anti-Human VIM Antibody Second Ab: 1μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu18)
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DAB staining on IHC-P;
Sample: Human Breast cancer Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human VIM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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Western Blot; Sample: Lane1: Hela cell lysate; Lane2: 293T cell lysate; Lane3: Rat Heart lysate; Lane4: Porcine Heart lysate; Lane5: Canine Heart lysate; Lane6: Bovine Heart lysate; Lane7: Gallus Heart lysate Primary Ab: 0.2μg/ml Mouse Anti-Human VIM Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine A
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)VIM的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別VIM。
用法
Western blotting: 0.01-3μg/mL
Immunohistochemistry: 2-30μg/mL
Immunocytochemistry: 2-30μg/mL
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽(yáng)性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長(zhǎng)臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB040Hu02 | 波形蛋白(VIM)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB040Hu01 | 波形蛋白(VIM)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB040Hu01 | 波形蛋白(VIM)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB040Hu02 | 波形蛋白(VIM)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB040Hu01 | 波形蛋白(VIM)多克隆抗體 | WB; IHC; ICC/IF |
| LAB040Hu71 | 波形蛋白(VIM)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAB040Hu24 | 波形蛋白(VIM)單克隆抗體 | WB; IHC |
| MAB040Hu25 | 波形蛋白(VIM)單克隆抗體 | WB; IHC; IF |
| MAB040Hu21 | 波形蛋白(VIM)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB040Hu23 | 波形蛋白(VIM)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB040Hu27 | 波形蛋白(VIM)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB040Hu22 | 波形蛋白(VIM)單克隆抗體 | WB; IHC; ICC/IF |
| FAB040Hu02 | 抗波形蛋白(VIM)單克隆抗體 | Flow cytometry. |
| FAB040Hu06 | 抗波形蛋白(VIM)單克隆抗體 | Flow cytometry. |
| FAB040Hu04 | 抗波形蛋白(VIM)單克隆抗體 | Flow cytometry. |
| FAB040Hu08 | 抗波形蛋白(VIM)單克隆抗體 | Flow cytometry. |
| SEB040Hu | 波形蛋白(VIM)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB040Hu | 波形蛋白(VIM)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB040Hu01 | 波形蛋白(VIM)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Cancer?Epidemiology,?Biomarkers?& Prevention | Aberrant Vimentin Methylation Is Characteristic of Breast Cancer [pubmed:22315367] |
| Scientific Reports | Glyoxalase?1-knockdown?in?human?aortic?endothelial?cells - effect on the?proteome?andendothelial?function?estimates. [pubmed:27898103] |
| Journal of?Proteomics | HtrA3 is a cellular partner of cytoskeleton proteins and TCP1α chaperonin [Pubmed:29477555] |
| Journal of Biological Chemistry | Growth hormone induces Notch1 signaling in podocytes and contributes to proteinuria in diabetic nephropathy [Pubmed: 31511328] |
| American Journal of Transplantation | Extracellular vesicles derived from injured vascular tissue promote the formation of tertiary lymphoid structures in vascular allografts [Pubmed: 31729155] |
| Lung | Is Vimentin the Cause or Effect of Obstructive Sleep Apnea Development? [Pubmed: 32088750] |
| Cell Biol Toxicol | ZNF471 modulates EMT and functions as methylation regulated tumor suppressor with diagnostic and prognostic significance in cervical cancer [33566221] |
| CELL BIOLOGY AND TOXICOLOGY | Metastatic suppression by DOC2B is mediated by inhibition of epithelial-mesenchymal transition and induction of senescence [33758996] |
| Comparison of Vimentin Levels Between Preeclamptic and Normotensive Pregnant Women [] | |
| Advanced glycation end-products associate with podocytopathy in type II diabetic patients [] | |
| Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β?… [] |
