內脂素(VF)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)
ELISA Kit DIY Materials for Visfatin (VF)
Nampt; NAmPRTase; PBEF; PBEF1; Pre-B-Cell Colony Enhancing Factor; Nicotinamide Phosphoribosyltransferase
- 編號KSA638Hu11
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 試劑內容 包被抗體、生物素標記的競爭物、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理競爭抑制酶聯免疫吸附試驗法測抗原
- 可檢測范圍1.23-100ng/mL
- 可適靈敏度
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 4882 ¥ 8136 ¥ 14645 ¥ 28476 ¥ 48816
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特異性
試劑盒中的抗體對內脂素(VF)具有高靈敏度和高特異性,經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務
相關產品
編號 | 適用物種:Homo sapiens (Human,人) | 應用(僅供研究使用,不用于臨床診斷!) |
RPA638Hu01 | 內脂素(VF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
CPA638Hu21 | 內脂素(VF)卵白蛋白偶聯物 | Immunogen; SDS-PAGE; WB. |
PAA638Hu01 | 內脂素(VF)多克隆抗體 | WB; IHC |
PAA638Hu08 | 內脂素(VF)多克隆抗體 | WB; IHC; ICC; IP. |
FAA638Hu01 | 抗內脂素(VF)多克隆抗體 | Flow cytometry. |
MAA638Hu22 | 內脂素(VF)單克隆抗體 | WB |
MAA638Hu27 | 內脂素(VF)單克隆抗體 | WB; IHC; ICC; IP. |
MAA638Hu29 | 內脂素(VF)單克隆抗體 | WB; IHC; ICC; IP. |
MAA638Hu21 | 內脂素(VF)單克隆抗體 | WB |
MAA638Hu23 | 內脂素(VF)單克隆抗體 | WB; IHC; ICC; IP. |
MAA638Hu24 | 內脂素(VF)單克隆抗體 | WB; IHC |
MAA638Hu25 | 內脂素(VF)單克隆抗體 | WB; IHC; ICC; IP. |
MAA638Hu26 | 內脂素(VF)單克隆抗體 | WB; IHC; ICC; IP. |
FAA638Hu04 | 抗內脂素(VF)單克隆抗體 | Flow cytometry. |
FAA638Hu02 | 抗內脂素(VF)單克隆抗體 | Flow cytometry. |
FAA638Hu06 | 抗內脂素(VF)單克隆抗體 | Flow cytometry. |
FAA638Hu08 | 抗內脂素(VF)單克隆抗體 | Flow cytometry. |
SEA638Hu | 內脂素(VF)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEA638Hu | 內脂素(VF)檢測試劑盒(酶聯免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CEA638Hu | 內脂素(VF)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA638Hu | 內脂素(VF)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSA638Hu11 | 內脂素(VF)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
Brazilian Journal of Biomoricity | EFFECT OF 8 WEEKS ENDURANCE TRAINING ON PLASMA VISFATIN IN MIDDLE-AGED MEN [Brjb: source] |
Diabetes Research and Clinical Practice | The relationship between visfatin and HOMA-IR in hypertensive patients, and the effect of antihypertensive drugs on visfatin and HOMA-IR in hypertensive patients with insulin resistance [ScienceDirect: S0168822711002956] |
J Physiol Pharmacol. | Early-effect of bariatric surgery (Scopinaro method) on intestinal hormones and adipokines in insulin resistant Wistar rat. [Pubmed: 24304571] |
Endocr J. | CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes [Pubmed:25168658] |
Exp Ther Med. | Plasma visfatin levels and mRNA expression of visfatin in peripheral blood mononuclear cells and peripheral blood monocyte-derived macrophages from normal weight females with polycystic ovary syndrome [Pubmed:Pmc3991522] |
Eur J Cancer Prev. | Visfatin derived from ascites promotes ovarian cancer cell migration through Rho/ROCK signaling-mediated actin polymerization. [Pubmed:25055182] |
Genome | Visfatin ?948G/T?and resistin ?420C/Gpolymorphisms in Egyptian type 2 diabetic patients with and without cardiovascular diseases [Nrcresearchpress:Source] |
PLOS ONE | Antidiabetic Effect of Galantamine: Novel Effect for a Known Centrally Acting Drug [PubMed: 26262991] |
Alcoholism and Drug Addiction | Changes in concentration of visfatin during four weeks of inpatient treatment of alcohol dependent males [Science: Article] |
Cytokine | Variation in blood levels of hormones in obese patients following weight reduction induced by endoscopic and surgical bariatric therapies [PubMed: 26539806] |
Genome | Genetic Variations in Visfatin and Resistin Genes in Egyptian Type 2 Diabetic Patients With and Without Complications [PubMed: 25120107] |
Acta Histochemica | Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines [science:S0065128116301234] |
Endocrine, Metabolic & Immune Disorders-Drug Targets | Changes in Serum Nampt Levels and Its Significances in Diabetic Nephropathy Patients-The Potential Role of Nampt in T2DM with Diabetic Nephropathy. [pubmed:28699485] |
Endocrine?Connections | Post-chemotherapy serum anti-müllerian hormone level predicts ovarian function recovery [Pubmed:30012648] |
Bioscience Reports | Effect of maternal hypothyroidism during pregnancy on insulin resistance, lipid accumulation and mitochondrial dysfunction in skeletal muscle of fetal rats [Pubmed:29784871] |
Contraception | Hyperinsulinemic-euglycemic clamp over the first year of use of depot-medroxyprogesterone acetate as a contraceptive [Pubmed:29665358] |
Cranio?-?Journal?of?Craniomandibular?Practice | Analysis of synovial fluid visfatin level in temporomandibular joint disorders [Pubmed:29385907] |
Nutrire | Resistin and visfatin concentrations are related to central obesity and inflammation in Brazilian children [10.1186:s41110-018-0060-7] |
DNA?and?Cell?Biology | Transcriptional Regulation of?NAMPT?Gene by Glycogen Synthase Kinase 3β in Goat Adipocytes [Pubmed: 30615481] |
Jundishapur?Journal of?Chronic?Disease?Care | The Effect of 8 Weeks Aquatic Exercise Program on Plasma Visfatin Level and Some Blood Factors in Obese Men [] |
Biomarkers in Heart Failure and Associated Diseases | Adipokines as Biomarkers in Health and Disease [] |
Journal of Translational Medicine | Plasma concentration and expression of adipokines in epicardial and subcutaneous adipose tissue are associated with impaired left ventricular filling pattern [Pubmed: 31533725] |
clinical neurology and neurosurgery | Relationsh?p of spontaneous subarachnoid haemorrhage and cerebral aneurysm to serum Visfatin and Nesfatin-1 levels [Pubmed: 32311618] |
The Horizon of Medical Sciences | Effects of 10-Week Concurrent Training on Insulin Resistance and the Serum Levels of Vaspin and Visfatin in Overweight Females [] |
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY | Different Ratios of Corn and Coconut Oil Blends in High?\Fat Diets Influence Fat Deposition without Altering Metabolic Biomarkers in Male Rats [] |
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