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內(nèi)脂素(VF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit for Visfatin (VF)

Nampt; NAmPRTase; PBEF; PBEF1; Pre-B-Cell Colony Enhancing Factor; Nicotinamide Phosphoribosyltransferase

  • 內(nèi)脂素(VF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 內(nèi)脂素(VF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 內(nèi)脂素(VF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) 實(shí)驗(yàn)結(jié)果圖
  • SEA638Hu.jpg 標(biāo)準(zhǔn)曲線圖
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒用于檢測(cè)內(nèi)脂素(VF)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的內(nèi)脂素(VF)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 93-104 99
EDTA plasma(n=5) 99-105 102
heparin plasma(n=5) 79-103 98

精密度

精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的內(nèi)脂素(VF)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中內(nèi)脂素(VF)含量的測(cè)定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 80-102% 97-104% 79-101% 93-101%
EDTA plasma(n=5) 78-102% 98-105% 90-101% 85-103%
heparin plasma(n=5) 91-103% 90-97% 92-101% 80-94%

穩(wěn)定性

經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。

實(shí)驗(yàn)流程

1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測(cè)溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測(cè)溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。

實(shí)驗(yàn)原理

將內(nèi)脂素(VF)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的內(nèi)脂素(VF)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的內(nèi)脂素(VF)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過(guò)氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的內(nèi)脂素(VF)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(O.D.值)，計(jì)算樣品濃度。

相關(guān)產(chǎn)品

編號(hào) 適用物種：Homo sapiens (Human，人) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA638Hu01 內(nèi)脂素(VF)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
CPA638Hu21 內(nèi)脂素(VF)卵白蛋白偶聯(lián)物 Immunogen; SDS-PAGE; WB.
PAA638Hu01 內(nèi)脂素(VF)多克隆抗體 WB; IHC
PAA638Hu08 內(nèi)脂素(VF)多克隆抗體 WB; IHC; ICC; IP.
FAA638Hu01 抗內(nèi)脂素(VF)多克隆抗體 Flow cytometry.
MAA638Hu22 內(nèi)脂素(VF)單克隆抗體 WB
MAA638Hu27 內(nèi)脂素(VF)單克隆抗體 WB; IHC; ICC; IP.
MAA638Hu29 內(nèi)脂素(VF)單克隆抗體 WB; IHC; ICC; IP.
MAA638Hu21 內(nèi)脂素(VF)單克隆抗體 WB
MAA638Hu23 內(nèi)脂素(VF)單克隆抗體 WB; IHC; ICC; IP.
MAA638Hu24 內(nèi)脂素(VF)單克隆抗體 WB; IHC
MAA638Hu25 內(nèi)脂素(VF)單克隆抗體 WB; IHC; ICC; IP.
MAA638Hu26 內(nèi)脂素(VF)單克隆抗體 WB; IHC; ICC; IP.
FAA638Hu04 抗內(nèi)脂素(VF)單克隆抗體 Flow cytometry.
FAA638Hu02 抗內(nèi)脂素(VF)單克隆抗體 Flow cytometry.
FAA638Hu06 抗內(nèi)脂素(VF)單克隆抗體 Flow cytometry.
FAA638Hu08 抗內(nèi)脂素(VF)單克隆抗體 Flow cytometry.
SEA638Hu 內(nèi)脂素(VF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA638Hu 內(nèi)脂素(VF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA638Hu 內(nèi)脂素(VF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA638Hu 內(nèi)脂素(VF)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA638Hu11 內(nèi)脂素(VF)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Brazilian Journal of Biomoricity EFFECT OF 8 WEEKS ENDURANCE TRAINING ON PLASMA VISFATIN IN MIDDLE-AGED MEN [Brjb: source]
Diabetes Research and Clinical Practice The relationship between visfatin and HOMA-IR in hypertensive patients, and the effect of antihypertensive drugs on visfatin and HOMA-IR in hypertensive patients with insulin resistance [ScienceDirect: S0168822711002956]
J Physiol Pharmacol. Early-effect of bariatric surgery (Scopinaro method) on intestinal hormones and adipokines in insulin resistant Wistar rat. [Pubmed: 24304571]
Endocr J. CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes [Pubmed:25168658]
Exp Ther Med. Plasma visfatin levels and mRNA expression of visfatin in peripheral blood mononuclear cells and peripheral blood monocyte-derived macrophages from normal weight females with polycystic ovary syndrome [Pubmed:Pmc3991522]
Eur J Cancer Prev. Visfatin derived from ascites promotes ovarian cancer cell migration through Rho/ROCK signaling-mediated actin polymerization. [Pubmed:25055182]
Genome Visfatin ?948G/T?and resistin ?420C/Gpolymorphisms in Egyptian type 2 diabetic patients with and without cardiovascular diseases [Nrcresearchpress:Source]
PLOS ONE Antidiabetic Effect of Galantamine: Novel Effect for a Known Centrally Acting Drug [PubMed: 26262991]
Alcoholism and Drug Addiction Changes in concentration of visfatin during four weeks of inpatient treatment of alcohol dependent males [Science: Article]
Cytokine Variation in blood levels of hormones in obese patients following weight reduction induced by endoscopic and surgical bariatric therapies [PubMed: 26539806]
Genome Genetic Variations in Visfatin and Resistin Genes in Egyptian Type 2 Diabetic Patients With and Without Complications [PubMed: 25120107]
Acta Histochemica Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines [science:S0065128116301234]
Endocrine, Metabolic & Immune Disorders-Drug Targets Changes in Serum Nampt Levels and Its Significances in Diabetic Nephropathy Patients-The Potential Role of Nampt in T2DM with Diabetic Nephropathy. [pubmed:28699485]
Endocrine?Connections Post-chemotherapy serum anti-müllerian hormone level predicts ovarian function recovery [Pubmed:30012648]
Bioscience Reports Effect of maternal hypothyroidism during pregnancy on insulin resistance, lipid accumulation and mitochondrial dysfunction in skeletal muscle of fetal rats [Pubmed:29784871]
Contraception Hyperinsulinemic-euglycemic clamp over the first year of use of depot-medroxyprogesterone acetate as a contraceptive [Pubmed:29665358]
Cranio?-?Journal?of?Craniomandibular?Practice Analysis of synovial fluid visfatin level in temporomandibular joint disorders [Pubmed:29385907]
Nutrire Resistin and visfatin concentrations are related to central obesity and inflammation in Brazilian children [10.1186:s41110-018-0060-7]
DNA?and?Cell?Biology Transcriptional Regulation of?NAMPT?Gene by Glycogen Synthase Kinase 3β in Goat Adipocytes [Pubmed: 30615481]
Jundishapur?Journal of?Chronic?Disease?Care The Effect of 8 Weeks Aquatic Exercise Program on Plasma Visfatin Level and Some Blood Factors in Obese Men []
Biomarkers in Heart Failure and Associated Diseases Adipokines as Biomarkers in Health and Disease []
Journal of Translational Medicine Plasma concentration and expression of adipokines in epicardial and subcutaneous adipose tissue are associated with impaired left ventricular filling pattern [Pubmed: 31533725]
clinical neurology and neurosurgery Relationsh?p of spontaneous subarachnoid haemorrhage and cerebral aneurysm to serum Visfatin and Nesfatin-1 levels [Pubmed: 32311618]
The Horizon of Medical Sciences Effects of 10-Week Concurrent Training on Insulin Resistance and the Serum Levels of Vaspin and Visfatin in Overweight Females []
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY Different Ratios of Corn and Coconut Oil Blends in High?\Fat Diets Influence Fat Deposition without Altering Metabolic Biomarkers in Male Rats []
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