干擾素α(IFNa)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Interferon Alpha (IFNa)
IFNA1; IFL; LeIF D; IFN; IFN-Alpha; IFNA13; IFN-A; IFNAP22; IFN-Alpha 1b; Interferon Alpha 1b; Interferon, Leukocytic; IFN, Leukocyte; Interferon alpha-D
- 編號SEA033Eq
- 物種Equus caballus; Equine (Horse，馬) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍15.6-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于6.1pg/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 3251 ¥ 4644 ¥ 20898 ¥ 39474 ¥ 325080
- 欲了解實際交易價格和更多情況，請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
實驗結(jié)果圖
-
標(biāo)準(zhǔn)曲線圖
-
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測干擾素α(IFNa)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的干擾素α(IFNa)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 93-101 | 97 |
| EDTA plasma(n=5) | 78-89 | 86 |
| heparin plasma(n=5) | 80-90 | 87 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的干擾素α(IFNa)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中干擾素α(IFNa)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-95% | 96-104% | 80-98% | 92-105% |
| EDTA plasma(n=5) | 90-99% | 89-104% | 88-98% | 96-103% |
| heparin plasma(n=5) | 82-89% | 79-95% | 90-99% | 97-104% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將干擾素α(IFNa)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的干擾素α(IFNa)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的干擾素α(IFNa)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的干擾素α(IFNa)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Equus caballus; Equine (Horse，馬) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| EPA033Eq61 | 干擾素α(IFNa)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA033Eq01 | 干擾素α(IFNa)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA033Eq01 | 干擾素α(IFNa)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA033Eq21 | 干擾素α(IFNa)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA033Eq | 干擾素α(IFNa)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| IEA033Eq | 干擾素α(IFNa)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA033Eq | 干擾素α(IFNa)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA033Eq | 干擾素α(IFNa)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Journal of Animal and Veterinary Advances | Tryptophan Stimulates Immune Response in Broiler Chickens Challenged with Infectious Bursal Disease Vaccine [Medwell: 2010610616] |
| Journal of Animal and Veterinary Advances | Immunostimulatory Effects of Arginine in Broiler Chickens Challenged with Vaccine Strain of Infectious Bursal Disease Virus [Medwell: 2010594600] |
| Ann Rheum Dis. | The cathelicidins LL-37 and rCRAMP are associated with pathogenic events of arthritis in humans and rats [PubMed: 23172753] |
| Nanomedicine | Efficient intracellular delivery of siRNA with a safe multitargeted lipid-based nanoplatform [PubMed: 23394132] |
| International journal of experimental pathology | Experimental rat model for alcohol-induced osteonecrosis of the femoral head [PubMed: PMC3781777] |
| Antiviral Research | A recombinant adenovirus bicistronically expressing porcine interferon-α and interferon-γ enhances antiviral effects against foot-and-mouth disease virus [ScienceDirect: S0166354214000266] |
| British Journal of Pharmacology | Andrographolide exerts anti-hepatitis C virus activity by up-regulating haeme oxygenase-1 via the p38 MAPK/Nrf2 pathway in human hepatoma cells [Onlinelibrary: bph.12440] |
| BMC Cancer | High efficiency of alphaviral gene transfer in combination with 5-fluorouracil in a mouse mammary tumor model [Pubmed:24950740] |
| PLoS One. | Respiratory Mechanics and Plasma Levels of Tumor Necrosis Factor Alpha and Interleukin 6 Are Affected by Gas Humidification during Mechanical Ventilation in Dogs [Pubmed:25036811] |
| Veterinary Research | Infection of equine monocyte-derived macrophages with an attenuated equine infectious anemia virus (EIAV) strain induces a strong resistance to the infection by a virulent EIAV strain [Pubmed:25106750] |
| Asian Pac J Trop Med | Effect of SecinH3 on lung injury induced by sepsis of rats [PubMed: 26706678] |
| PLoS?One | siRNA Targeting the 2A pro Genomic Region Prevents Enterovirus 71 Replication In Vitro [Pubmed:26886455] |
| Plos One | Sulforaphane?Suppresses?Hepatitis?C?Virus?Replication?by?Up-ReCavia (Guinea pig )lating?Heme?Oxygenase-1?Expressionthrough?PI3K/Nrf2?Pathway. [Pubmed:27023634] |
| PLoS One. | Vaccination with recombinant Mycobacterium tuberculosis PknD attenuates bacterial dissemination to the brain in Cavia (Guinea pig )inea pigs. [Pubmed:23776655] |
| Veterinary microbiology | Impact of porcine reproductive and respiratory syndrome virus and porcine circovirus-2 infection on the potency of the classical swine fever vaccine (LOM strain). [pubmed:27599928] |
| International Journal of?Clinical?and Experimental Pathology | Host miR-146a promotes replication of human cytomegalovirus by suppressing type I IFN response in MRC-5 cells [files:ijcep0036479.pdf] |
| Scandinavian?Journal?of?Immunology | Serum?Proteomics?Analysis?in?Rats?of?Immunosuppression?Induced?by?Chronic?Stress. [pubmed:27384769] |
| Saudi Journal of Gastroenterology | Deoxyschizandrin?suppresses?dss-induced?ulcerative?colitis?in?mice. [pubmed:27976641] |
| Biochemical and Biophysical Research Communications | MicroRNA-548j inhibits type I interferon production by targeting ZBTB11 in patients with chronic hepatitis B [pubmed:28342861] |
| Virology | The down-regulation of casein kinase 1 alpha as a host defense response against infectious bursal disease virus infection [pubmed:28988058] |
| Antiviral Research | Coinjection of a vaccine and anti-viral agents can provide fast-acting protection from foot-and-mouth disease [pubmed:28454913] |
| Molecular Therapy. Methods & Clinical Development | Anti-high mobility group box 1 antibody ameliorates albuminuria in MRL/lprlupus-prone mice [pubmed:28649578] |
| Journal of Virology | Fucoidan from Fucus vesiculosus suppresses hepatitis B virus replication by enhancing extracellular signal-regulated Kinase activation. [pubmed:28915824] |
| Clinical?Immunology | Toll-like receptor 9 ligands increase type I interferon induced B-cell activating factor expression in chronic rhinosinusitis with nasal polyposis [Pubmed:30056130] |
| International?forum?of?allergy?& rhinology | Role of Toll‐like receptor 9 signaling on activation of nasal polyp–derived fibroblasts and its association with nasal polypogenesis [Pubmed:29901280] |
| LUPUS | Collagen triple helix repeat containing-1: a novel biomarker associated with disease activity in Systemic lupus erythematosus [Pubmed: 30336754] |
| Journal of?Pineal?Research | Role of melatonin in sleep deprivation‐induced intestinal barrier dysfunction in mice [Pubmed: 30929267] |
| Veterinary Immunology and Immunopathology | Differential expression of type I interferon mRNA and protein levels induced by virulent Marek's disease virus infection in chickens [] |
| international immunopharmacology | Adenovirus-mediated down-regulation of miR-21-5p alleviates experimental autoimmune uveoretinitis in mice [Pubmed: 31228813] |
| FASEB Journal | Scavenger receptor A impairs interferon response to HBV infection by limiting TRAF 3 ubiquitination through recruiting OTUB 1 [] |
| Transboundary and Emerging Diseases | Pathogenicity of porcine reproductive and respiratory syndrome virus (ORF5 RFLP 1–7–4 viruses) in China [Pubmed: 32187856] |
| archives of virology | A flagellin-adjuvanted inactivated porcine epidemic diarrhea virus (PEDV) vaccine provides enhanced immune protection against PEDV challenge in piglets [Pubmed: 32253616] |
| Autophagy | LDHB inhibition induces mitophagy and facilitates the progression of CSFV infection [Pubmed: 32924761] |
| THERIOGENOLOGY | Porcine soluble CD83 alleviates LPS-induced abortion in mice by promoting Th2 cytokine production, Treg cell generation and trophoblast invasion [Pubmed: 32810792] |
| Int Immunopharmacol | BNIP3 deletion ameliorated enterovirus 71 infection-induced hand, foot and mouth disease via inhibiting apoptosis, autophagy, and inflammation in mice [Pubmed: 32717566] |
| Molecular Medicine Reports | Aquaporin?4 deletion ameliorates enterovirus 71 infection in mice [Pubmed: 32582978] |
| Virulence | Determination of antiviral action of Long Non-coding RNA loc107051710 During Infectious Bursal Disease Virus Infection due to Enhancement of Interferon?… [] |
| РОССИЙСКАЯ СЕЛЬСКОХОЗЯЙСТВЕННАЯ НАУКА | ГОРМОНАЛЬНЫЙ И ЦИТОКИНОВЫЙ ПРОФИЛЬ КРОВИ МОЛОЧНЫХ КОРОВ В РАННИЙ ПЕРИОД ГЕСТАЦИИ [] |
| Communications Biology | Influenza virus neuraminidase regulates host CD8+ T-cell response in mice [33293641] |
| Animals | Concentration-Dependent Type 1 Interferon-Induced Regulation of MX1 and FABP3 in Bovine Endometrial Explants [33494350] |
| Int J Dermatol | IFN?\|á and TNF?\|á serum levels and their association with disease severity in Egyptian children and adults with alopecia areata [34008204] |
| Dig Dis Sci | Orexin A Suppresses the Expression of Exosomal PD-L1 in Colon Cancer and Promotes T Cell Activity by Inhibiting JAK2/STAT3 Signaling Pathway [34097168] |
| Clinical findings of Talaromyces marneffei infection among patients with anti-interferon-|? immunodeficiency: a prospective cohort study [34144700] | |
| ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | Chronic developmental exposure to low-dose ([C8mim][PF6]) induces neurotoxicity and behavioural abnormalities in rats [34555716] |
| Carbohydrate Polymers | The anti-inflammatory activity of specific-sized hyaluronic acid oligosaccharides [34823813] |
| Poult Sci | Effects of different selenium sources on duodenum and jejunum tight junction network and growth performance of broilers in a model of fluorine-induced?… [Pubmed:35066382] |
| Veterinary Microbiology | Self-assembled BPIV3 nanoparticles can induce comprehensive immune responses and protection against BPIV3 challenge by inducing dendritic cell maturation in?… [Pubmed:35395543] |
留言咨詢
