G蛋白偶聯雌激素受體1(GPER)檢測試劑盒(酶聯免疫吸附試驗法)
ELISA Kit for G Protein Coupled Estrogen Receptor 1 (GPER)
GPR30; CMKRL2; DRY12; FEG-1; GPCR-Br; LERGU; LyGPR; Membrane estrogen receptor; Chemoattractant receptor-like 2; Flow-induced endothelial G-protein coupled receptor 1
- 編號SEG045Mu
- 物種Mus musculus (Mouse,小鼠) 相同的名稱,不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍0.312-20ng/mL
- 靈敏度最小可檢測劑量小于等于0.114ng/mL.
- 樣本類型Tissue homogenates, cell lysates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
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特異性
本試劑盒用于檢測G蛋白偶聯雌激素受體1(GPER),經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
精密度
精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
批內差: CV<10%
批間差: CV<12%
穩(wěn)定性
經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備;
2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
4. 洗板3次;
5. 加檢測溶液B100µL,37°C孵育30分鐘;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分鐘;
8. 加終止液50µL,立即450nm讀數。
實驗原理
將G蛋白偶聯雌激素受體1(GPER)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的G蛋白偶聯雌激素受體1(GPER)與連接于固相載體上的抗體結合,然后加入生物素化的G蛋白偶聯雌激素受體1(GPER)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的G蛋白偶聯雌激素受體1(GPER)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。
增值服務
相關產品
編號 | 適用物種:Mus musculus (Mouse,小鼠) | 應用(僅供研究使用,不用于臨床診斷!) |
RPG045Mu01 | G蛋白偶聯雌激素受體1(GPER)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPG045Mu02 | G蛋白偶聯雌激素受體1(GPER)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAG045Mu01 | G蛋白偶聯雌激素受體1(GPER)多克隆抗體 | WB; IHC; ICC; IP. |
PAG045Mu02 | G蛋白偶聯雌激素受體1(GPER)多克隆抗體 | WB; IHC; ICC; IP. |
SEG045Mu | G蛋白偶聯雌激素受體1(GPER)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMG045Mu | G蛋白偶聯雌激素受體1(GPER)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
雜志 | 參考文獻 |
The Journal of Steroid Biochemistry and Molecular Biology | Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR30 signalling [Pubmed:24846829] |
Molecular and Cellular Endocrinology | Isomer-nonspecific action of dichlorodiphenyltrichloroethane on aryl hydrocarbon receptor and G-protein-coupled receptor 30 intracellular signaling in apoptotic neuronal cells [Pubmed:24859647] |
J Steroid Biochem Mol Biol | Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR32 signalling [PubMed: 24846831] |
Journal of Environmental Sciences | Bisphenol A exposure alters release of immune and developmental modulators and expression of estrogen receptors in human fetal lung fibroblasts [science:S1001074216300924] |
Psychiatry research | Serum levels of G protein-coupled estrogen receptor 1 (GPER1) in drug-naive patients with generalized anxiety disorder. [pubmed:27512921] |
Clinical Psychopharmacology Neuroscience. | Increased Serum G Protein-coupled Estrogen Receptor 1 Levels and Its Diagnostic Value in Drug Na?ve Patients with Major Depressive Disorder [pubmed:29073745] |
Psychiatry?Research | Decreased levels of G protein-coupled estrogen receptor in children with autism spectrum disorders. [pubmed:28734238] |
Neuropsychiatric disease and treatment | serum levels of gPer-1 in euthymic bipolar patients [Pubmed:29618927] |
European archives of oto-rhino-laryngology | An evaluation of G-protein coupled membrane estrogen receptor-1 level in stuttering [Pubmed:29299745] |
Bosnian Journal of?Basic?Medical?Sciences | Evaluation of estrogen and G protein-coupled estrogen receptor 1 (GPER) levels in drug-na?ve patients with attention deficit hyperactivity disorder (ADHD) [Pubmed:29659348] |
Carleton University Research Virtual Environment | In Vitro Study of Mechanisms Underlying the Developmental Effects of Bisphenol A Using Human Fetal Lung Fibroblasts [] |
Am J Otolaryngol | GPER-1 and sex-hormone levels in patients with otosclerosis [Pubmed: 32144019] |
Andrologia. | Relationship between the G protein–coupled oestrogen receptor and spermatogenesis, and its correlation with male infertility [Pubmed: 32776559] |
Journal of Radiation Research and Applied Sciences | GPER-1 in chronic hepatitis B [] |
FASEB J | Endocrine disruption in Crohn's disease: Bisphenol A enhances systemic inflammatory response in patients with gut barrier translocation of dysbiotic microbiota??- [34085740] |
Comparing the Impact of Serum GPER-1 and Oxidant/Antioxidant Levels on Retinopathy in Diabetic Patients and Healthy Individuals: A Pilot Study [] | |
Horm Mol Biol Clin Investig | The change pattern in serum G protein-coupled estrogen receptor-1 (GPER1) levels during pregnancy with and without gestational diabetes mellitus [34787384] |