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G蛋白偶聯雌激素受體1(GPER)檢測試劑盒(酶聯免疫吸附試驗法)

ELISA Kit for G Protein Coupled Estrogen Receptor 1 (GPER)

GPR30; CMKRL2; DRY12; FEG-1; GPCR-Br; LERGU; LyGPR; Membrane estrogen receptor; Chemoattractant receptor-like 2; Flow-induced endothelial G-protein coupled receptor 1

  • G蛋白偶聯雌激素受體1(GPER)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
  • G蛋白偶聯雌激素受體1(GPER)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
  • G蛋白偶聯雌激素受體1(GPER)檢測試劑盒(酶聯免疫吸附試驗法) 實驗結果圖
  • SEG045Mu.jpg 標準曲線圖
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

本試劑盒用于檢測G蛋白偶聯雌激素受體1(GPER),經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

精密度

精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
批內差: CV<10%
批間差: CV<12%

穩(wěn)定性

經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本的準備;
2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
4. 洗板3次;
5. 加檢測溶液B100µL,37°C孵育30分鐘;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分鐘;
8. 加終止液50µL,立即450nm讀數。

實驗原理

將G蛋白偶聯雌激素受體1(GPER)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的G蛋白偶聯雌激素受體1(GPER)與連接于固相載體上的抗體結合,然后加入生物素化的G蛋白偶聯雌激素受體1(GPER)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的G蛋白偶聯雌激素受體1(GPER)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

相關產品

編號 適用物種:Mus musculus (Mouse,小鼠) 應用(僅供研究使用,不用于臨床診斷!)
RPG045Mu01 G蛋白偶聯雌激素受體1(GPER)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPG045Mu02 G蛋白偶聯雌激素受體1(GPER)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAG045Mu01 G蛋白偶聯雌激素受體1(GPER)多克隆抗體 WB; IHC; ICC; IP.
PAG045Mu02 G蛋白偶聯雌激素受體1(GPER)多克隆抗體 WB; IHC; ICC; IP.
SEG045Mu G蛋白偶聯雌激素受體1(GPER)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMG045Mu G蛋白偶聯雌激素受體1(GPER)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻

雜志 參考文獻
The Journal of Steroid Biochemistry and Molecular Biology Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR30 signalling [Pubmed:24846829]
Molecular and Cellular Endocrinology Isomer-nonspecific action of dichlorodiphenyltrichloroethane on aryl hydrocarbon receptor and G-protein-coupled receptor 30 intracellular signaling in apoptotic neuronal cells [Pubmed:24859647]
J Steroid Biochem Mol Biol Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR32 signalling [PubMed: 24846831]
Journal of Environmental Sciences Bisphenol A exposure alters release of immune and developmental modulators and expression of estrogen receptors in human fetal lung fibroblasts [science:S1001074216300924]
Psychiatry research Serum levels of G protein-coupled estrogen receptor 1 (GPER1) in drug-naive patients with generalized anxiety disorder. [pubmed:27512921]
Clinical Psychopharmacology Neuroscience. Increased Serum G Protein-coupled Estrogen Receptor 1 Levels and Its Diagnostic Value in Drug Na?ve Patients with Major Depressive Disorder [pubmed:29073745]
Psychiatry?Research Decreased levels of G protein-coupled estrogen receptor in children with autism spectrum disorders. [pubmed:28734238]
Neuropsychiatric disease and treatment serum levels of gPer-1 in euthymic bipolar patients [Pubmed:29618927]
European archives of oto-rhino-laryngology An evaluation of G-protein coupled membrane estrogen receptor-1 level in stuttering [Pubmed:29299745]
Bosnian Journal of?Basic?Medical?Sciences Evaluation of estrogen and G protein-coupled estrogen receptor 1 (GPER) levels in drug-na?ve patients with attention deficit hyperactivity disorder (ADHD) [Pubmed:29659348]
Carleton University Research Virtual Environment In Vitro Study of Mechanisms Underlying the Developmental Effects of Bisphenol A Using Human Fetal Lung Fibroblasts []
Am J Otolaryngol GPER-1 and sex-hormone levels in patients with otosclerosis [Pubmed: 32144019]
Andrologia. Relationship between the G protein–coupled oestrogen receptor and spermatogenesis, and its correlation with male infertility [Pubmed: 32776559]
Journal of Radiation Research and Applied Sciences GPER-1 in chronic hepatitis B []
FASEB J Endocrine disruption in Crohn's disease: Bisphenol A enhances systemic inflammatory response in patients with gut barrier translocation of dysbiotic microbiota??- [34085740]
Comparing the Impact of Serum GPER-1 and Oxidant/Antioxidant Levels on Retinopathy in Diabetic Patients and Healthy Individuals: A Pilot Study []
Horm Mol Biol Clin Investig The change pattern in serum G protein-coupled estrogen receptor-1 (GPER1) levels during pregnancy with and without gestational diabetes mellitus [34787384]
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