Bcl2關(guān)聯(lián)X蛋白(Bax)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Bcl2 Associated X Protein (Bax)
BCL2L4; Bax Zeta; Apoptosis regulator BAX; Bcl-2-like protein 4
- 編號SEB343Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍62.5-4,000pg/mL
- 靈敏度最小可檢測劑量小于等于28.2pg/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 2793 ¥ 3990 ¥ 17955 ¥ 33915 ¥ 279300
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測Bcl2關(guān)聯(lián)X蛋白(Bax)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的Bcl2關(guān)聯(lián)X蛋白(Bax)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 93-105 | 96 |
| EDTA plasma(n=5) | 86-94 | 90 |
| heparin plasma(n=5) | 79-92 | 86 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的Bcl2關(guān)聯(lián)X蛋白(Bax)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中Bcl2關(guān)聯(lián)X蛋白(Bax)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 83-92% | 80-96% | 78-92% | 99-105% |
| EDTA plasma(n=5) | 94-102% | 80-98% | 85-99% | 94-101% |
| heparin plasma(n=5) | 91-103% | 90-97% | 88-95% | 93-104% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將Bcl2關(guān)聯(lián)X蛋白(Bax)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的Bcl2關(guān)聯(lián)X蛋白(Bax)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的Bcl2關(guān)聯(lián)X蛋白(Bax)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的Bcl2關(guān)聯(lián)X蛋白(Bax)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB343Hu02 | Bcl2關(guān)聯(lián)X蛋白(Bax)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB343Hu01 | Bcl2關(guān)聯(lián)X蛋白(Bax)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPB343Hu21 | Bcl2關(guān)聯(lián)X蛋白(Bax)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAB343Hu01 | Bcl2關(guān)聯(lián)X蛋白(Bax)多克隆抗體 | WB; IHC; ICC; IP. |
| MAB343Hu22 | Bcl2關(guān)聯(lián)X蛋白(Bax)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB343Hu | Bcl2關(guān)聯(lián)X蛋白(Bax)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB343Hu | Bcl2關(guān)聯(lián)X蛋白(Bax)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| International Journal of Health Sciences | Neurotoxic effect of lead on rats: Relationship to Apoptosis [NCBI: PMC3883608] |
| Interactive cardiovascular and thoracic surgery | Effects of hepatocyte growth factor in myocarditis rats induced by immunization with porcine cardiac myosin [Pubmed: 24327573] |
| Biological Journal of Armenia | Cu (II) (PICOLINYL-L-TRYPTOPHAN)2 AS AN EFFECIENT MULTIFUNCTIONAL RADIOPROTECTOR [Asj-oa: Source] |
| Histochem Cell Biol. | Inhibition of small HA fragment activity and stimulation of A2A adenosine receptor pathway limit apoptosis and reduce cartilage damage in experimental arthritis [Pubmed:25511416] |
| Mol Cell Biochem. | The combined treatment with novel platinum(II) complex and anti-MUC1 increases apoptotic response in MDA-MB-231 breast cancer cells [PubMed: 26112902] |
| Histochem Cell Biol | Inhibition of small HA fragment activity and stimulation of A2A [PubMed: 25511416] |
| European Journal of Medicinal Plants | Hepatoprotective Effects of Rubus idaeus L. Leaves against CCl4-Induced Liver Injury via Antioxidant, Anti-inflammatory and Antiapoptotic Mechanisms [openview:626440] |
| Scientific Reports | In Vivo Characterization of ARN14140, a Memantine/Galantamine-Based Multi-Target Compound for Alzheimer's Disease [pubmed:27609215] |
| IOS Press Content Library | An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … [articles:journal-of-alzheimers-disease] |
| Behavioural Brain Research | Amyloid toxicity is enhanced after pharmacological or genetic invalidation of the 1 receptor [pubmed:29129596] |
| Biomedicine & Pharmacotherapy | Mechanistic insights into the effects of quercetin and/or GLP-1 analogue liraglutide on high-fat diet/streptozotocin-induced type 2 diabetes in rats [pubmed:28554128] |
| Oncology Letters | Mechanism of anticancer action of novel berenil complex of platinum(II) combined with anti-MUC1 in MCF-7 breast cancer cells [10.3892/ol.2017.7623] |
| International Journal of Biological Macromolecules | A polysaccharide (PNPA) from Pleurotus nebrodensis ameliorates hepatic ischemic/reperfusion (I/R) injury in rats [pubmed:28709897] |
| Toxicology and Environmental Health Sciences | Potentiation of Apoptosis in Rat Striatum Exposed to Endosulfan and the Role of Quercetin [10.1007/s13530-017-0325-1] |
| Biomedicine & Pharmacotherapy | Cardioprotective effect of the xanthones from Gentianella acuta against myocardial ischemia/reperfusion injury in isolated rat heart [pubmed:28686977] |
| Journal of Ethnopharmacology | Viscum articulatum Burm. f. aqueous extract exerts antiproliferative effect and induces cell cycle arrest and apoptosis in leukemia cells [Pubmed:29555410] |
| Oncology?Letters | Mechanism of anticancer action of novel berenil complex of platinum (II) combined with anti-MUC1 in MCF-7 breast cancer cells [Pubmed:29434943] |
| Scientific Research | Association of Bax and Bcl-2 Functional Polymorphisms and Protein Levels with Posttraumatic Stress Disorder [paperID:82598] |
| PeerJ. | Taxifolin protects rat against myocardial ischemia/reperfusion injury by modulating the mitochondrial apoptosis pathway [Pubmed: 30723634] |
| 博士論文 | INSTITUTO DE CIENCIAS BáSICAS Y PRECLíNICAS “VICTORIA DE GIRóN” DEPARTAMENTO DE HISTOLOGíA [] |
| Experimental Cell Research | LncRNA SNHG3 promotes clear cell renal cell carcinoma proliferation and migration by upregulating TOP2A [Pubmed: 31505165] |
| Toxicology | LCZ696 (sacubitril/valsartan) protects against cyclophosphamide-induced testicular toxicity in rats: Role of neprilysin inhibition and lncRNA TUG1 in ameliorating … [Pubmed: 32197949] |
| Odontology | Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells [Pubmed: 33206337] |
| Environ Sci Pollut Res Int | Potentiation of the apoptotic signaling pathway in both the striatum and hippocampus and neurobehavioral impairment in rats exposed chronically to a low? dose of?… [Pubmed: 32915453] |
| Drug Chem Toxicol | Alogliptin attenuates cyclophosphamide-induced nephrotoxicity: a novel therapeutic approach through modulating MAP3K/JNK/SMAD3 signaling cascade [Pubmed: 32869669] |
| Folia Biologica | Effect of Graviola (Annona Muricata l.) and Ginger (Zingiber Officinale Roscoe) on Diabetes Mellitus Induced in Male Wistar Albino Rats [] |
| J Biochem Mol Toxicol | Potential effect of adrenomedullin on metabolic and endocrinal dysfunctions in the experimentally induced polycystic ovary: Targeting implication of endoplasmic ?- [33491863] |
| Crystals | Potential Therapeutic Effects of New Ruthenium (III) Complex with Quercetin: Characterization, Structure, Gene Regulation, and Antitumor and Anti-Inflammatory Studies (RuIII/Q Novel Complex Is a Potent Immunoprotective Agent) [] |
| Biomed Pharmacother | Allicin ameliorates renal ischemia/reperfusion injury via inhibition of oxidative stress and inflammation in rats [34426252] |
| Environ Sci Pollut Res Int | Galangin mitigates oxidative stress, inflammation, and apoptosis in a rat model of methotrexate hepatotoxicity [34729716] |
| Life Sci | Selenium nanoparticles overcomes sorafenib resistance in thioacetamide induced hepatocellular carcinoma in rats by modulation of mTOR, NF-κB pathways and?… [Pubmed:35640776] |
| Life Sci | Piracetam attenuates cyclophosphamide-induced hepatotoxicity in rats: Amelioration of necroptosis, pyroptosis and caspase-dependent apoptosis [Pubmed:35636581] |
