神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Glial Fibrillary Acidic Protein (GFAP)
Intermediate Filament Protein
- 編號SEA068Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長3h
- 檢測范圍0.156-10ng/mL
- 靈敏度最小可檢測劑量小于等于0.060ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 2646 ¥ 3780 ¥ 17010 ¥ 32130 ¥ 264600
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 79-101 | 85 |
| EDTA plasma(n=5) | 81-92 | 85 |
| heparin plasma(n=5) | 83-95 | 91 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 82-89% | 97-104% | 81-98% | 87-104% |
| EDTA plasma(n=5) | 96-105% | 96-103% | 94-105% | 92-99% |
| heparin plasma(n=5) | 86-95% | 99-105% | 78-98% | 88-101% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計(jì)算樣品濃度。
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相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA068Hu05 | 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA068Hu02 | 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA068Hu04 | 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA068Hu01 | 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA068Hu01 | 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)多克隆抗體 | WB; IHC; FCM |
| PAA068Hu02 | 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA068Hu71 | 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| LAA068Hu81 | 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| MAA068Hu22 | 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA068Hu | 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEA068Hu | 抗神經(jīng)膠質(zhì)纖維酸性蛋白抗體(Anti-GFAP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| LMA068Hu | 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA068Hu01 | 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | 自己動(dòng)手制作ELISA試劑盒的主要材料 |
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