欧美综合自拍亚洲综合网_欧美黑人巨大videos异族_可乐操中文字幕_亚洲成av人福利专区网站_国产强奸视频在播放_免费无码国产在线53_最近最新的免費中文字幕_抖音免费视频素材_亚洲精品熟女一二区_国产无套粉嫩白浆在线麻豆

磷脂酰肌醇蛋白聚糖3(GPC3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit for Glypican 3 (GPC3)

DGSX; OCI5; SDYS; SGB; SGBS1; MXR7; Glypican Proteoglycan 3; GTR2-2; Intestinal protein OCI-5; Secreted glypican-3

  • 磷脂酰肌醇蛋白聚糖3(GPC3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 磷脂酰肌醇蛋白聚糖3(GPC3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 磷脂酰肌醇蛋白聚糖3(GPC3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) 實(shí)驗(yàn)結(jié)果圖
  • SEA971Hu.jpg 標(biāo)準(zhǔn)曲線圖
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒用于檢測磷脂酰肌醇蛋白聚糖3(GPC3)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的磷脂酰肌醇蛋白聚糖3(GPC3)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 78-99 95
EDTA plasma(n=5) 90-104 94
heparin plasma(n=5) 98-105 101

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的磷脂酰肌醇蛋白聚糖3(GPC3)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中磷脂酰肌醇蛋白聚糖3(GPC3)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 79-101% 78-94% 82-89% 88-98%
EDTA plasma(n=5) 88-101% 78-94% 94-105% 78-96%
heparin plasma(n=5) 93-101% 90-104% 87-94% 87-97%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。

實(shí)驗(yàn)流程

1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。

實(shí)驗(yàn)原理

將磷脂酰肌醇蛋白聚糖3(GPC3)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的磷脂酰肌醇蛋白聚糖3(GPC3)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的磷脂酰肌醇蛋白聚糖3(GPC3)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的磷脂酰肌醇蛋白聚糖3(GPC3)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計(jì)算樣品濃度。

相關(guān)產(chǎn)品

編號 適用物種：Homo sapiens (Human，人) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA971Hu02 磷脂酰肌醇蛋白聚糖3(GPC3)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA971Hu02 磷脂酰肌醇蛋白聚糖3(GPC3)活性蛋白 Cell?culture;?Activity?Assays.
RPA971Hu01 磷脂酰肌醇蛋白聚糖3(GPC3)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA971Hu01 磷脂酰肌醇蛋白聚糖3(GPC3)活性蛋白 Cell?culture;?Activity?Assays.
PAA971Hu01 磷脂酰肌醇蛋白聚糖3(GPC3)多克隆抗體 WB; ICC; IHC-P; IHC-F; ELISA.
MAA971Hu21 磷脂酰肌醇蛋白聚糖3(GPC3)單克隆抗體 WB; IHC; ICC; IP.
MAA971Hu22 磷脂酰肌醇蛋白聚糖3(GPC3)單克隆抗體 WB; IHC; ICC; IP.
MAA971Hu24 磷脂酰肌醇蛋白聚糖3(GPC3)單克隆抗體 WB; IHC; ICC; IP.
MAA971Hu23 磷脂酰肌醇蛋白聚糖3(GPC3)單克隆抗體 WB; IHC; ICC; IP.
MAA971Hu25 磷脂酰肌醇蛋白聚糖3(GPC3)單克隆抗體 WB; IHC; ICC; IP.
FAA971Hu82 抗磷脂酰肌醇蛋白聚糖3(GPC3)單克隆抗體(異硫氰酸熒光素標(biāo)記) Flow cytometry.
SEA971Hu 磷脂酰肌醇蛋白聚糖3(GPC3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA971Hu 磷脂酰肌醇蛋白聚糖3(GPC3)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Nature and Science Diagnostic Role of Serum Glypican-3 as a Tumor Marker for Hepatocellular Carcinoma [Sciencepub:10043238]
Journal of Applied Pharmaceutical Science Cytotoxic effects of antiglypican-3 against HepG2 cell line [Ebscohost: Source]
Asian Pacific Journal of Cancer Prevention Can Glypican3 be Diagnostic for Early Hepatocellular Carcinoma among Egyptian Patients? [Pubmed: 24460300]
Clin Biochem. Diagnostic accuracy of serum glypican-3 for hepatocellular carcinoma: A systematic review and meta-analysis [Pubmed: 24362268]
Science and Nature Diagnostic Role of Serum Glypican-3 as Hepatocellular Carcinoma [Zolcat: Source]
Alexandria Journal of Medicine Role of serum glypican-3 in the diagnosis and differentiation of small hepatocellular carcinoma from hepatitis-C virus cirrhosis [ScienceDirect: S2090506814000062]
J Egypt Natl Canc Inst. Comparing prothrombin induced by vitamin K absence-II (PIVKA-II) with the oncofetal proteins glypican-3, Alpha feto protein and carcinoembryonic antigen in diagnosing hepatocellular carcinoma among Egyptian patients. [Pubmed:24841158]
European Journal of Pharmacology Evaluation of antiglypican-3 therapy as a promising target for amelioration of hepatic tissue damage in hepatocellular carcinoma [Pubmed:25449037]
Hepatobiliary Pancreat Dis Int Predictive value of tumor markers in patients with recurrent hepatocellular carcinoma in different vascular invasion pattern [abstract:abstract4525.shtml]
Clinical?and Molecular Hepatology? Glypican-3 level assessed by the enzyme-linked immunosorbent assay is inferior to alpha-fetoprotein level for hepatocellular carcinoma diagnosis [pmc:articles]
Wiley Glypican‐3: A promising biomarker for hepatocellular carcinoma diagnosis and treatment [pubmed:28621802]
Biomedicine & Pharmacotherapy Cytotoxic and partial hepatoprotective activity of sodium ascorbate against hepatocellular carcinoma through inhibition of sulfatase-2 and [Pubmed:29669302]
Journal of Cancer and Clinical Oncology Development and Validation of a Two-Site Immunoradiometric assay for Glypican-3 in plasma: Implication in Diagnosis of Hepatocellular Carcinoma [Researchgate:Source]
Turkish?journal?of?oncology Serum Glypican-3, Vascular Endothelial Growth Factor, and Interleukin-6 Levels in Hepatocellular Carcinoma [10.5505:tjo.2018.1742]
Intensive?Care?Medicine?Experimental Elevated plasma glypicans are associated with organ failure in patients with infection [Pubmed: 30618011]
VALUE OF SERUM GLYPICAN 3 AS A PREDICTIVE TOOL FOR EARLY DIAGNOSIS OF HEPATOCELLULAR CARCINOMA (HCC) []
ADVANCED RESEARCHES Search for Effective Serum Tumor Markers for Early Diagnosis of Hepatocellular Carcinoma Associated with Hepatitis C []
Life Sci Selenium nanoparticles overcomes sorafenib resistance in thioacetamide induced hepatocellular carcinoma in rats by modulation of mTOR, NF-κB pathways and?… [Pubmed:35640776]
留言咨詢