髓鞘堿性蛋白(MBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Myelin Basic Protein (MBP)
Myelin A1 protein; Myelin membrane encephalitogenic protein
- 編號SEA539Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍0.156-10ng/mL
- 靈敏度最小可檢測劑量小于等于0.065ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cerebrospinal fluid, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 2646 ¥ 3780 ¥ 17010 ¥ 32130 ¥ 264600
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測髓鞘堿性蛋白(MBP)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的髓鞘堿性蛋白(MBP)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 85-92 | 89 |
| EDTA plasma(n=5) | 90-97 | 94 |
| heparin plasma(n=5) | 91-103 | 101 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的髓鞘堿性蛋白(MBP)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中髓鞘堿性蛋白(MBP)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 93-103% | 89-97% | 92-99% | 94-105% |
| EDTA plasma(n=5) | 80-95% | 79-103% | 99-105% | 93-105% |
| heparin plasma(n=5) | 86-97% | 84-95% | 93-103% | 80-102% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將髓鞘堿性蛋白(MBP)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的髓鞘堿性蛋白(MBP)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的髓鞘堿性蛋白(MBP)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的髓鞘堿性蛋白(MBP)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA539Hu04 | 髓鞘堿性蛋白(MBP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA539Hu03 | 髓鞘堿性蛋白(MBP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA539Hu02 | 髓鞘堿性蛋白(MBP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA539Hu61 | 髓鞘堿性蛋白(MBP)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA539Hu01 | 髓鞘堿性蛋白(MBP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA539Hu61 | 髓鞘堿性蛋白(MBP)活性蛋白 | Cell?culture;?Activity?Assays. |
| CPA539Hu21 | 髓鞘堿性蛋白(MBP)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA539Hu22 | 髓鞘堿性蛋白(MBP)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA539Hu23 | 髓鞘堿性蛋白(MBP)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA539Hu01 | 髓鞘堿性蛋白(MBP)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA539Hu02 | 髓鞘堿性蛋白(MBP)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA539Hu52 | 髓鞘堿性蛋白(MBP)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA539Hu07 | 髓鞘堿性蛋白(MBP)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA539Hu08 | 髓鞘堿性蛋白(MBP)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA539Hu09 | 髓鞘堿性蛋白(MBP)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA539Hu06 | 髓鞘堿性蛋白(MBP)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA539Hu71 | 髓鞘堿性蛋白(MBP)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAA539Hu22 | 髓鞘堿性蛋白(MBP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA539Hu21 | 髓鞘堿性蛋白(MBP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA539Hu26 | 髓鞘堿性蛋白(MBP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA539Hu23 | 髓鞘堿性蛋白(MBP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA539Hu28 | 髓鞘堿性蛋白(MBP)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA539Hu | 髓鞘堿性蛋白(MBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA539Hu | 髓鞘堿性蛋白(MBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA539Hu | 髓鞘堿性蛋白(MBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEA539Hu | 抗髓鞘堿性蛋白抗體(Anti-MBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| SCA539Hu | 髓鞘堿性蛋白(MBP)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA539Hu | 髓鞘堿性蛋白(MBP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA539Hu01 | 髓鞘堿性蛋白(MBP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| PLOS ONE | CD98 Positive Eosinophils Contribute to T Helper 1 Pattern Inflammation [PlosOne: Source] |
| Journal of Occupational Health | Effects of Exposure to 1-Bromopropane on Astrocytes and Oligodendrocytes in Rat Brain [Pubmed: 23183024] |
| Pak J Med Sci | Myelin basic protein and ischemia modified albumin levels in acute ischemic stroke cases [PubMed: 26648996] |
| Experimental and Toxicologic Pathology | Morphology and staining behavior of neutrophilic and eosinophilic granulocytes of the common marmoset (Callithrix jacchus) [Pubmed:27165445] |
| Acta Angiologica | Serum microtubule associated protein tau and myelin basic protein as the potential markers of brain ischaemia-reperfusion injury in patients undergoing carotid endarterectomy [AA.2016.0008] |
| Stem Cells. | Myelin Basic Protein Regulates Primitive and Definitive Neural Stem Cell Proliferation from the Adult Spinal Cord. [pubmed:27573615] |
| Environmental Science and Pollution Research | Glial?markers?and?emotional?memory?in?rats?following?acute?cerebral?radiofrequency?exposures. [pubmed:27696165] |
| Acta Angiol | Serum microtubule associated protein tau and myelin basic protein as the potential markers of brain ischaemia-reperfusion injury in patients undergoing carotid endarterectomy [AA.2016.0008] |
| PLoS?Neglected?Tropical?Diseases | Functional and phenotypic evaluation of eosinophils from patients with the acute form of paracoccidioidomycosis [pubmed:28489854] |
| Pesticide Biochemistry and Physiology | Acrolein-mediated neurotoxicity in growing Wistar male rats [Pubmed:30033014] |
| International Journal of Livestock Research | Association and Risk of Canine Distemper with Respect to Age, Sex and Breed of Dogs Suffering from Demyelinating Neuropathies [10.5455:ijlr.20170811080455] |
| Microchimica Acta | Biosensors and nanobiosensors for rapid detection of autoimmune diseases: a review [Pubmed: 31760523] |
| The role of light desynchronosis in the development of stress-induced aging [] | |
| Adv Med Sci | Association of eosinophil-mediated inflammatory biomarkers with the presence of the Schatzki ring [34091432] |
| biomolecules | Searching for Noninvasive Predictors of the Diagnosis and Monitoring of Eosinophilic Esophagitis?aThe Importance of Biomarkers of the Inflammatory Reaction??- [] |
| Talanta | Monitoring autoimmune diseases by bioelectrochemical detection of autoantibodies. Application to the determination of anti-myelin basic protein autoantibodies in?… [Pubmed:35202838] |
