環(huán)加氧酶2(COX 2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Cyclooxygenase-2 (COX 2)
PGG/HS; PGHS2; PHS2; HCox2; PTGS2; Prostaglandin Endoperoxide Synthase 2; Prostaglandin G/H Synthase And Cyclooxygenase; Prostaglandin H2 synthase 2
- 編號SEA699Hu
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍0.312-20ng/mL
- 靈敏度最小可檢測劑量小于等于0.114ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 2646 ¥ 3780 ¥ 17010 ¥ 32130 ¥ 264600
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特異性
本試劑盒用于檢測環(huán)加氧酶2(COX 2),經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制,不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測,因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的環(huán)加氧酶2(COX 2)(加標(biāo)樣品),重復(fù)測定并計算其均值,回收率為測定值與理論值的比率。
樣本 | 回收率范圍(%) | 平均回收率(%) |
serum(n=5) | 95-104 | 101 |
EDTA plasma(n=5) | 80-92 | 85 |
heparin plasma(n=5) | 93-101 | 96 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復(fù)測定8次,分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的環(huán)加氧酶2(COX 2),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中環(huán)加氧酶2(COX 2)含量的測定值與理論值的比率。
樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 89-97% | 81-96% | 93-101% | 98-105% |
EDTA plasma(n=5) | 89-103% | 79-103% | 90-97% | 84-99% |
heparin plasma(n=5) | 78-91% | 92-102% | 82-101% | 86-98% |
穩(wěn)定性
經(jīng)測定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備;
2. 加樣(標(biāo)準(zhǔn)品及樣本)100µL,37°C孵育1小時;
3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
4. 洗板3次;
5. 加檢測溶液B100µL,37°C孵育30分鐘;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分鐘;
8. 加終止液50µL,立即450nm讀數(shù)。
實驗原理
將環(huán)加氧酶2(COX 2)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本,其中的環(huán)加氧酶2(COX 2)與連接于固相載體上的抗體結(jié)合,然后加入生物素化的環(huán)加氧酶2(COX 2)抗體,將未結(jié)合的生物素化抗體洗凈后,加入HRP標(biāo)記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色,并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的環(huán)加氧酶2(COX 2)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPA699Hu02 | 環(huán)加氧酶2(COX 2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA699Hu03 | 環(huán)加氧酶2(COX 2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA699Hu01 | 環(huán)加氧酶2(COX 2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA699Hu01 | 環(huán)加氧酶2(COX 2)多克隆抗體 | WB,IHC |
PAA699Hu02 | 環(huán)加氧酶2(COX 2)多克隆抗體 | WB; IHC; ICC; IP. |
MAA699Hu22 | 環(huán)加氧酶2(COX 2)單克隆抗體 | WB; IHC; ICC; IP. |
SEA699Hu | 環(huán)加氧酶2(COX 2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA699Hu | 環(huán)加氧酶2(COX 2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
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