可羅索(KL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Klotho (KL)
KLa; Klotho Alpha
- 編號SEH757Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍0.156-10ng/mL
- 靈敏度最小可檢測劑量小于等于0.063ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 3192 ¥ 4560 ¥ 20520 ¥ 38760 ¥ 319200
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測可羅索(KL)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的可羅索(KL)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 82-92 | 86 |
| EDTA plasma(n=5) | 85-92 | 89 |
| heparin plasma(n=5) | 79-97 | 86 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的可羅索(KL)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中可羅索(KL)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 91-98% | 98-105% | 81-96% | 83-103% |
| EDTA plasma(n=5) | 96-103% | 83-90% | 85-96% | 96-103% |
| heparin plasma(n=5) | 78-90% | 85-102% | 82-102% | 85-101% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將可羅索(KL)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的可羅索(KL)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的可羅索(KL)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的可羅索(KL)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APH757Ra01 | 可羅索(KL)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPH757Ra02 | 可羅索(KL)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPH757Ra01 | 可羅索(KL)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAH757Ra02 | 可羅索(KL)多克隆抗體 | WB; IHC; ICC; IP. |
| PAH757Ra01 | 可羅索(KL)多克隆抗體 | WB; IHC; ICC; IP. |
| MAH757Ra21 | 可羅索(KL)單克隆抗體 | WB; IHC; ICC; IP. |
| SEH757Ra | 可羅索(KL)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMH757Ra | 可羅索(KL)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Life Sciences | High phosphate feeding induced arterial medial calcification in uremic rats: Roles of Lanthanum carbonate on protecting vasculature [Pubmed: 24012609] |
| EXPERIMENTAL & CLINICAL CARDIOLOGY | Sick Sinus Syndrome May Attribute to the Decrease of ?±-klotho Protein in Mice [Com:Source] |
| Int J Clin Exp Pathol.? | α-Klotho is an acute phase protein and altered by restraint stress in mice [Pubmed:Pmc4203207] |
| Annales de Biologie Clinique | Les biomarqueurs des calcifications vasculaires [PubMed: 26069069] |
| Mol Med Rep | Effect of secondary hyperparathyroidism serum on endothelial cells and intervention with Klotho [PubMed: 25873020] |
| Calcif Tissue Int | Human Preosteoblastic Cell Culture from a Patient with Severe Tumoral Calcinosis-Hyperphosphatemia Due to a NewGALNT3 [PubMed: 25899975] |
| 中華內(nèi)分泌代謝雜志 | 抗衰老蛋白Klotho 阻斷高糖培養(yǎng)的大鼠腎小球系膜細(xì)胞TLR4/NF-κB p65/NGAL 通路的激活及其作用機(jī)制 [Cn: Article] |
| Mol Cell Endocrinol | The function of miR-199a-5p/Klotho regulating TLR4/NF-κB p65/NGAL pathways in rat mesangial cells cultured with high glucose and the mechanism [PubMed: 26419931] |
| Arch Med Sci | Soluble Klotho protein as a novel serum biomarker in patients with acromegaly [pmc:PMC4754356] |
| Am J Nephrol | Klotho?Deficiency?Aggravates?Tacrolimus-Induced?Renal?Injury?via?the?Phosphatidylinositol?3-Kinase-Akt-Forkhead?Box?Protein?O?Pathway [Pubmed:27174564] |
| Sci Rep. | Klotho preservation via histone deacetylase inhibition attenuates chronic kidney disease-associated bone injury in mice. [pubmed:28387374] |
| Nephrology | Is there a role for newer biomarkers in chronic kidney disease-mineral and bone disorder management? [pubmed:28429560] |
| Journal of Integrative Nephrology & Andrology | Associations between serum fibroblast growth factor 23 level and intradialytic hypotension in hemodialysis patients [issn:2394-2916] |
| International Journal of Clinical and Experimental Pathology | Protective effect of restructuring klotho protein combined with trimetazidine on myocardial injury in mice [ISSN:1936-2625] |
| Journal of veterinary medical science | Changes of Klotho protein and Klotho mRNA expression in a hydroxy-L-proline induced hyperoxaluric rat model [pubmed:28943597] |
| Cellular Physiology and Biochemistry | Klotho Reduces Necroptosis by Targeting Oxidative Stress Involved in Renal Ischemic-Reperfusion Injury [Pubmed:29550818] |
| American Journal of Translational?Research | Advanced maternal age impairs spatial learning capacity in young adult mouse offspring [Pubmed:29636887] |
| Journal of?Diabetes?and its?Complications | Clinical implication of alterations in serum Klotho levels in patients with type 2 diabetes mellitus and its associated complications [Pubmed:30042059] |
| Medical?Science?Monitor | Klotho Inhibits Proliferation and Migration of Angiotensin II-Induced Vascular Smooth Muscle Cells (VSMCs) by Modulating NF-κB p65, Akt, and Extracellular Signal?… [Pubmed:30004089] |
| Nephrology Dialysis?Transplantation | High-serum phosphate and parathyroid hormone distinctly regulate bone loss and vascular calcification in experimental chronic kidney disease [Pubmed: 30189026] |
| BMC?Nephrology | The role of klotho in chronic kidney disease [Pubmed: 30348110] |
| Nature?Communications | Age-related declines in α-Klotho drive progenitor cell mitochondrial dysfunction and impaired muscle regeneration [Pubmed: 30451844] |
| Kidney?International | Wnt/β-catenin signaling mediates both heart and kidney injury in type 2 cardiorenal syndrome [Pubmed: 30770217] |
| Expert?Opinion on?Therapeutic?Targets | The use of LP533401 as a therapeutic option for renal osteodystrophy affects, renal calcium handling, vitamin D metabolism and bone health in uremic rats [Pubmed: 30801205] |
| Mol Immunol | CD317 mediates immunocytolysis resistance by RICH2/cytoskeleton-dependent membrane protection [Pubmed: 33223223] |
| Journal of Clinical Hypertension | Effects of salt intervention on serum levels of Klotho influenced by salt sensitivity [Pubmed: 33164306] |
| CURRENT EYE RESEARCH | Expression of α-Klotho is downregulated and associated with oxidative stress in the lens in streptozotocin-induced diabetic rats [Pubmed: 32744464] |
| international immunopharmacology | Klotho ameliorates the onset and progression of cataract via suppressing oxidative stress and inflammation in the lens in streptozotocin-induced diabetic rats [Pubmed: 32438076] |
| Purinergic Signalling | P2X7 siRNA targeted to the kidneys increases klotho and delays the progression of experimental diabetic nephropathy. [Pubmed: 32377919] |
| Oxidative Medicine and Cellular Longevity | Ameliorating Effect of Klotho Protein on Rat Heart during I/R Injury [Pubmed: 33123314] |
| NEUROSCIENCE | Increased Plasma Level of Longevity Protein Klotho as a Potential Indicator of Cognitive Function Preservation in Patients With Schizophrenia [Pubmed: 32612508] |
| Pharmacological Reports | |áKlotho protein has therapeutic activity in contrast-induced acute kidney injury by limiting NLRP3 inflammasome-mediated pyroptosis and promoting autophagy [33675964] |
| J Mol Neurosci | Investigation of the Role of Induced Overexpression of the Isolated Secreted Klotho on the A-172 Human Glioblastoma Cells [Pubmed:35112226] |
| Reproductive Biology and Endocrinology | Expression of Klotho in Follicles of Patients With Polycystic Ovary Syndrome and Its Clinical Significance [] |
| Journal of Ovarian Research | Klotho is a prognostic indicator of oocytes qualities in polycystic ovary syndrome [] |
